Will the Amniotic Membrane and its Stem Cells Preserve their Capacity After Cryopreservation?

Khayat-Khoei, Mahsa

doi:10.4172/2157-7099.1000e118

Cited by 1 publication

(2 citation statements)

References 13 publications

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“…Also, because of their greater self-renewal ability which differentiates MSCs from other sources, and for a paucity of study on these types of cells, we studied them from this source [15][16][17][18]. In recent years, according to the biological roles and specialized capacities of stem cells and amniotic membrane, special attention has been paid to their use in both the medical and clinical settings [11,19]. AM membrane consists of a thick base membrane layer and an avascular stroma layer; there are two types of stem cells in this membrane, AESCs, and AM-MSC.…”

Section: Introductionmentioning

confidence: 99%

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Studying the suppressing effect of mesenchymal stem cells derived from amniotic membrane on colorectal cancer

2021

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Mesenchymal stem cell therapy is considered as a proper tool for biological activities and treatment of diseases and cancer. Here, the suppressing effect of mesenchymal stem cells derived from the amniotic membrane (AM-MSCs) on colorectal cancer (HT29 cell line) was studied. MSCs were isolated from the human amniotic membrane and identity tests were performed. AM-MSC and HT29 cells were cocultured and MTT assay was used to determine proper inhibitory concentration. The apoptotic effect of AM-MSC on HT29 cells was studied by Acridine orange staining. Expression of CDK2 protein and the antioxidant effect of AM-MSCs on HT29 was performed by immunocyto chemistry test. The effect of MSC on the HT29 cell line migration was determined by Scratch test. The result showed that AM-MSC had proliferation-inhibitory effects and caused apoptosis of the HT29 cell line. Real Time-PCR assay showed the increase of gene expression of p53, Caspase3 in apoptosis, and p21 in tumor cell line treated with AM-MSC. AM-MSC arrested the cell cycle of tumor cells by reduction of CDK2 protein. The migration of HT29 and ROS expression levels was decreased. Altogether, AM-MSC may play a role in suppressing colorectal cancer HT29 cell line by inhibiting angiogenesis, cell cycle, and induction of apoptosis and has antioxidant properties.

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Section: Introductionmentioning

confidence: 99%

“…AM-MSCs are located in an avascular stroma. AM membrane is considered to be a postpartum tissue, which can be used for cell therapy due to its antiinflammatory properties, low immunity, antimicrobial, anti-apoptotic, and anti-angiogenesis properties [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%