Wild-Type Yellow Fever Virus RNA in Cerebrospinal Fluid of Child

Marinho, Paula Eillanny Silva; Alvarenga, Pedro Paulo Martins; Crispim, Ana Paula Correia; Candiani, Talitah Michel Sanchez; Alvarenga, Alice Martins; Bechler, Isabela M; Alves, Pedro Augusto; Dornas, Fábio P.; Oliveira, Danilo Bretas de; Bentes, Aline Almeida; Christo, Paulo Pereira; Kroon, Erna Geessien

doi:10.3201/eid2508.181479

Cited by 20 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our observations may not conclusively demonstrate that ILHV infection led to the brain hemorrhage and death in a patient with an acute neurological syndrome and underlying conditions (diabetes and hypertension). Considering the increasing incidence of arboviruses with unusual manifestations ( [51][52][53] and reviewed in [54][55][56]), and their potential tropism for the invasion of the CNS, leading to long-term neurofunctional sequelae, this report highlights the need for vigilance among physicians, healthcare providers, and researchers alike for arbovirus infections in patients presenting with meningoencephalitis and cerebrovascular events. Furthermore, this study demonstrates the importance of comprehensive arbovirus surveillance beyond urban arboviruses (e.g., DENV, CHIKV, and ZIKV), suggesting that urban and peri-urban populations may be at risk for ILHV infection and other emerging zoonotic arboviruses.…”

Section: Discussionmentioning

confidence: 98%

Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis

Milhim

Estofolete

Rocha

et al. 2020

Viruses

View full text Add to dashboard Cite

Ilheus virus is an arbovirus with the potential for central nervous system involvement. Accurate diagnosis is a challenge due to similar clinical symptoms and serologic cross-reactivity with other flaviviruses. Here, we describe the first documented case of a fatal outcome following the identification of Ilheus virus in the cerebrospinal fluid (CSF) of a patient with cerebral encephalitis in Brazil.

show abstract

Section: Discussionmentioning

confidence: 98%

Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis

Milhim

Estofolete

Rocha

et al. 2020

Viruses

View full text Add to dashboard Cite

show abstract

“…The patient had mildly elevated amino transaminases but no classical YF signs or symptoms. After six days of hospitalization, the child died, and postmortem analysis showed wild-type YFV RNA in the cerebrospinal fluid [29]. The persistence of the wild-type YFV genome has been demonstrated in serum [28] and in urine [28,30], until 28 and 47 days after the onset of symptoms, respectively.…”

Section: Main Textmentioning

confidence: 99%

Recent sylvatic yellow fever virus transmission in Brazil: the news from an old disease

Silva

Sacchetto

Rezende

et al. 2020

Virol J

141

166

View full text Add to dashboard Cite

Yellow fever (YF) is an acute viral disease, affecting humans and non-human primates (NHP), caused by the yellow fever virus (YFV). Despite the existence of a safe vaccine, YF continues to cause morbidity and mortality in thousands of people in Africa and South America. Since 2016, massive YF outbreaks have taken place in Brazil, reaching YF-free zones, causing thousands of deaths of humans and NHP. Here we reviewed the main epidemiological aspects, new clinical findings in humans, and issues regarding YFV infection in vectors and NHP in Brazil. The 2016-2019 YF epidemics have been considered the most significant outbreaks of the last 70 years in the country, and the number of human cases was 2.8 times higher than total cases in the previous 36 years. A new YFV lineage was associated with the recent outbreaks, with persistent circulation in Southeast Brazil until 2019. Due to the high number of infected patients, it was possible to evaluate severity and death predictors and new clinical features of YF. Haemagogus janthinomys and Haemagogus leucocelaenus were considered the primary vectors during the outbreaks, and no human case suggested the occurrence of the urban transmission cycle. YFV was detected in a variety of NHP specimens presenting viscerotropic disease, similar to that described experimentally. Further studies regarding NHP sensitivity to YFV, YF pathogenesis, and the duration of the immune response in NHP could contribute to YF surveillance, control, and future strategies for NHP conservation.

show abstract

“…In addition to these well-known neurotropic flaviviruses, there has been an increasing number of reported cases of neurological complications caused by viruses, such as dengue virus (DENV) and yellow fever virus (YFV), which usually are associated with hemorrhagic disease [6,7].…”

Section: Introductionmentioning

confidence: 99%

Modelling Neurotropic Flavivirus Infection in Human Induced Pluripotent Stem Cell-Derived Systems

Desole

Sinigaglia

Riccetti

et al. 2019

IJMS

View full text Add to dashboard Cite

Generation of human induced pluripotent stem cells (hiPSCs) and their differentiation into a variety of cells and organoids have allowed setting up versatile, non-invasive, ethically sustainable, and patient-specific models for the investigation of the mechanisms of human diseases, including viral infections and host–pathogen interactions. In this study, we investigated and compared the infectivity and replication kinetics in hiPSCs, hiPSC-derived neural stem cells (NSCs) and undifferentiated neurons, and the effect of viral infection on host innate antiviral responses of representative flaviviruses associated with diverse neurological diseases, i.e., Zika virus (ZIKV), West Nile virus (WNV), and dengue virus (DENV). In addition, we exploited hiPSCs to model ZIKV infection in the embryo and during neurogenesis. The results of this study confirmed the tropism of ZIKV for NSCs, but showed that WNV replicated in these cells with much higher efficiency than ZIKV and DENV, inducing massive cell death. Although with lower efficiency, all flaviviruses could also infect pluripotent stem cells and neurons, inducing similar patterns of antiviral innate immune response gene expression. While showing the usefulness of hiPSC-based infection models, these findings suggest that additional virus-specific mechanisms, beyond neural tropism, are responsible for the peculiarities of disease phenotype in humans.

show abstract

Wild-Type Yellow Fever Virus RNA in Cerebrospinal Fluid of Child

Cited by 20 publications

References 11 publications

Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis

Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis

Recent sylvatic yellow fever virus transmission in Brazil: the news from an old disease

Modelling Neurotropic Flavivirus Infection in Human Induced Pluripotent Stem Cell-Derived Systems

Contact Info

Product

Resources

About