We extend the study of lowest moments, x and x 2 , of the parton distribution function of the nucleon to include those of the sea quarks; this entails a disconnected insertion calculation in lattice QCD. This is carried out on a 16 3 × 24 quenched lattice with Wilson fermion. The quark loops are calculated with Z 2 noise vectors and unbiased subtractions, and multiple nucleon sources are employed to reduce the statistical errors. We obtain 5σ signals for x for the u, d, and s quarks, but x 2 is consistent with zero within errors.We provide results for both the connected and disconnected insertions. The perturbatively renormalizedx for the strange quark at µ = 2 GeV is x s+s = 0.027 ± 0.006 which is consistent with the experimental result. The ratio of x for s vs. u/d in the disconnected insertion with quark loops is calculated to be 0.88 ± 0.07. This is about twice as large as the phenomenologically fitted x s+s x ū + x d from experiments whereū andd include both the connected and disconnected insertion parts. We discuss the source and implication of this difference.