Widespread occurrence of extensive epimural rod bacteria in the hindguts of marine Thalassinidae and Brachyura (Crustacea: Decapoda)

Harris, Jean M.

doi:10.1007/bf00355480

Cited by 19 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This assumption is supported by several previous observations of hindguts of various arthropods, in which bacteria exhibiting morphologies or attachment preferences similar to those of the rodshaped bacteria from P. scaber were observed. Bacteria with almost identical morphologies were observed in the gut contents of shrimps (32) and termites (4), attached to the gut cuticle structures of the millipede (7) and marine decapod crustaceans (18), or forming rosette-like aggregations in the gut of the cockroach (5).…”

Section: Discussionmentioning

confidence: 99%

“CandidatusBacilloplasma,” a Novel Lineage ofMollicutesAssociated with the Hindgut Wall of the Terrestrial IsopodPorcellio scaber(Crustacea: Isopoda)

Kostanjšek

Štrus

Avguštin

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Pointed, rod-shaped bacteria colonizing the cuticular surface of the hindgut of the terrestrial isopod crustacean Porcellio scaber (Crustacea: Isopoda) were investigated by comparative 16S rRNA gene sequence analysis and electron microscopy. The results of phylogenetic analysis, and the absence of a cell wall, affiliated these bacteria with the class Mollicutes, within which they represent a novel and deeply branched lineage, sharing less than 82.6% sequence similarity to known Mollicutes. The lineage has been positioned as a sister group to the clade comprising the Spiroplasma group, the Mycoplasma pneumoniae group, and the Mycoplasma hominis group. The specific signature sequence was identified and used as a probe in in situ hybridization, which confirmed that the retrieved sequences originate from the attached rod-shaped bacteria from the hindgut of P. scaber and made it possible to detect these bacteria in their natural environment. Scanning and transmission electron microscopy revealed a spherically shaped structure at the tapered end of the rod-shaped bacteria, enabling their specific and exclusive attachment to the tip of the cuticular spines on the inner surface of the gut. Specific adaptation to the gut environment, as well as phylogenetic positioning, indicate the long-term association and probable coevolution of the bacteria and the host. Taking into account their pointed, rod-shaped morphology and their phylogenetic position, the name "Candidatus Bacilloplasma" has been proposed for this new lineage of bacteria specifically associated with the gut surface of P. scaber.The common woodlouse Porcellio scaber is a widely spread species of the terrestrial isopod crustaceans (Crustacea, Isopoda, Oniscidea) living in temperate climates. Like other terrestrial isopods, P. scaber is a herbivorous scavenger, feeding predominantly on decayed plant material and thus contributing to nutrient and energy cycling in terrestrial ecosystems (43). Due to its ecological importance, easy handling, breeding capability under laboratory conditions, and tolerance to polluted environments as well as the considerable body of knowledge about its biology, this crustacean is commonly used as a test organism in terrestrial ecotoxicological and ecophysiological studies (12,17).The tripartite digestive system of P. scaber consists of a short foregut comprising an esophagus and stomach, a midgut consisting of two pairs of blind-ended tubular digestive glands, and a long, tube-like hindgut. The last of these comprises two functionally different parts, an anterior chamber and a papillate region with a rectum (17). The foregut of terrestrial isopods is generally poorly inhabited by microorganisms. On the other hand, a high microbial density is found in the hindgut, particularly in the papillate region, where favorable conditions (44) allow the multiplication of those microorganisms that have survived the digestion in the anterior part of the digestive tract of P. scaber (24, 26) and those of other terrestrial isopods like Oniscus asellu...

show abstract

Section: Discussionmentioning

confidence: 99%

“CandidatusBacilloplasma,” a Novel Lineage ofMollicutesAssociated with the Hindgut Wall of the Terrestrial IsopodPorcellio scaber(Crustacea: Isopoda)

Kostanjšek

Štrus

Avguštin

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The presence of bacteria in the digestive tracts (particularly the hindgut) of Crustacea is widespread, occurring across the taxa, feeding types and habitats [28,29]. These associations range from pathogenic to mutualistic, and from facultative to obligate [30], but in general, the roles that these bacteria play in the nutrition of their hosts are unknown.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea Alvinocarid shrimp Rimicaris exoculata gut

Zbinden

Cambon-Bonavita

2003

FEMS Microbiology Ecology

View full text Add to dashboard Cite

The phylogenetic diversity of the gut microbial population of the hydrothermal shrimp Rimicaris exoculata was determined. The presence of micro-organisms in the hindgut of the shrimp was determined, and their morphology illustrated for the first time by transmission electron microscopy. DNA was extracted from the fore-, mid- and hindgut of shrimps collected at the Rainbow site, at the Mid-Atlantic Ridge, and the sequences of the small-subunit rRNA (16S rDNA) were analyzed. Seven different bacterial phylotypes were identified from the 90 clones screened. The sequences were affiliated to three major groups: epsilon-Proteobacteria (48,8%), Entomoplasmatales (23.3%) and representatives of the new phylum Deferribacteres (26.6%). These results show that the diversity in the shrimp gut is very low compared to that of the surrounding medium. Furthermore, the presence of groups that are not found in the external medium (Spiroplasma sp. and Geovibrio sp.) suggests the existence of a local microflora. The potential roles of these bacteria are discussed, involving the shrimp diet and metal bioremediation.

show abstract

“…Bacteria are well-known to be associated with digestive tracts of invertebrates (10,35,40,41), and bacteria are also known to be harbored within the hemolymph from several species of crustaceans (4,44). Prolific colonization of crustacean hindguts with bacteria with apparent monocultures may be common (17). The digestive tracts of Penaeus species have been reported to harbor a diverse commensal bacterial flora, including Vibrio, Pseudomonas, and Aeromonas species (49), and in paddy field-cultured prawns, including Penaeus mon-odon, non-O1 Vibrio cholerae appears to constitute normal microflora (27).…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon

Fuerst

Gwilliam

Lindsay

et al. 1997

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain Bacteria were isolated from postlarvae of the giant tiger prawn, Penaeus monodon. A selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. Planctomycetes were isolated from both healthy and monodon baculovirus-infected prawn postlarvae. The predominant colony type recovered from postlarvae regardless of viral infection status was nonpigmented. Other, less commonly observed types were pink or orange pigmented. A planctomycete-specific 16S rRNA-directed probe was designed and used to screen the isolates for their identity as planctomycetes prior to molecular phylogenetic characterization. 16S rRNA genes from nine prawn isolates together with two planctomycete reference strains (Planctomyces brasiliensis and Gemmata obscuriglobus) were sequenced and compared with reference sequences from the planctomycetes and other members of the domain Bacteria. Phylogenetic analyses and sequence signatures of the 16S rRNA genes demonstrated that the prawn isolates were members of the planctomycete group. Five representatives of the predominant nonpigmented colony type were members of the Pirellula group within the planctomycetes, as were three pink-pigmented colony type representatives. Homology values and tree topology indicated that representatives of the nonpigmented and pink-pigmented colony types formed two discrete clusters within the Pirellula group, not identical to any known Pirellula species. A sole representative of the orange colony type was a member of the Planctomyces group, virtually identical in 16S rDNA sequence to P. brasiliensis, and exhibited distinctive morphology.

show abstract

Widespread occurrence of extensive epimural rod bacteria in the hindguts of marine Thalassinidae and Brachyura (Crustacea: Decapoda)

Cited by 19 publications

References 38 publications

“CandidatusBacilloplasma,” a Novel Lineage ofMollicutesAssociated with the Hindgut Wall of the Terrestrial IsopodPorcellio scaber(Crustacea: Isopoda)

“CandidatusBacilloplasma,” a Novel Lineage ofMollicutesAssociated with the Hindgut Wall of the Terrestrial IsopodPorcellio scaber(Crustacea: Isopoda)

Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea Alvinocarid shrimp Rimicaris exoculata gut

Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon

Contact Info

Product

Resources

About