Widespread haploidy in monokaryotic cells of mature basidiocarps of Armillaria bulbosa, a member of the Armillaria mellea complex

Genetic Evidence for Somatic Haploidization in Developing Fruit Bodies of Armillaria tabescens

“…The results are in agreement with the cytological observations of Tommerup and Broadbent (1975) and Peabody and Peabody (1985).…”

A Genetic Mosaic in the Fruiting Stage of Armillaria gallica

“…Mated cells of Armillaria are predominantly monokaryotic and diploid, and it has long been assumed that basidiomes forming on them are also diploid because most basidiome cells are monokaryotic. However, we reported that A. gallica basidiomes collected in Bridgewater, Massachusetts, were monokaryotic and haploid, because no quantitative differences were found when the nuclear DNA content of haploid spores and three different basidiome cell types were compared (Peabody and Peabody, 1985). To determine whether this geographic isolate of A. gallica was atypical, eight additional isolates were sampled and basidiomes were all found to be monokaryotic and haploid (Peabody and Peabody, 1987).…”

Characterization of Saccharomyces cerevisiae mutants lacking the E1α subunit of the pyruvate dehydrogenase complex

“…Cells were harvested, washed and incubated for 30min at room temperature in McIlvaines buffer (0.1 M sodium citrate, 0.2 M NaH2P04, pH 4.4) containing 0.1 mg/l4',6-diamidino-2-phenylindole (Peabody and Peabody, 1985). Fluorescent-labeled cclls werc placed between coverslips and observed with a Zeiss Axioscop microscope, equipped with a 100-W high-pressure mercury lamp and a 100j1.3 plan-neofluor lens.…”