Widely targeted metabolomics analysis of different parts of kudzu

Abstract

Pueraria lobata is a traditional medicinal and edible plant. Its root is often used as a nutritional supplement, but its stems and leaves are often discarded. In this study, the types and contents of compounds in roots, stems and leaves of kudzu were studied by ultra‐high‐performance liquid chromatography electrospray ionization tandem mass spectrometry. A total of 446 metabolites were identified, which were mainly divided into eight categories; 40 unique compounds were detected in roots. Multivariate statistical analysis showed that there were significant differences in the contents of metabolites in the roots, stems and leaves of kudzu, and the most significant differences were found in the roots and stems. Most flavonoids and terpenoids accumulated in roots, while alkaloids, flavonoid glycosides, lignans and coumarins accumulated in stems and leaves. In addition, isoflavones accumulated least in stems and most in roots. These results improved our understanding of the accumulation of metabolites in kudzu and provided a reference for the full study of the medicinal value of different parts of kudzu.

Widely Targeted Metabolomics Analysis of Dendrobium officinale at Different Altitudes

“…[33 -35] Secondary metabolites in plants have important pharmacological roles, for example, the bibenzyl derivatives in D. officinale can be combined with SIRT3 directly, promote deacetylation and trigger manganese superoxide dismutase (Mn-SOD), thus protecting against UV-B-induced skin photoaging. [13] Alkaloids and flavonoids are protective against UVB-induced damage to human keratin-forming cells, [36] erianin can inhibit the proliferation of human Luminal A breast cancer cells, [37] and total flavonoids have a protective effect on mouse models of aging that are induced by D-galactose. [38] In addition, total polyphenols and total flavonoids in D. officinale have potential antioxidant activity.…”

“…It is based on QTRAP mass spectrometry in multiple reaction monitoring (MRM) mode and can quantify hundreds of known metabolites and nearly a thousand unknown metabolites simultaneously, with high throughput, sensitivity, qualitative accuracy, reproducibility and complete database availability. It has been used for the detection of metabolites of wild Ophiocordyceps sinensis, [10] Lyciumbarbarum fruits, [11] Rehmannia glutinosa, [12] Pueraria montana, [13] Morchella fruits, [14] etc.…”

Protein Tyrosine Phosphatase 1B Inhibitors of Pueraria lobata Based on the Spectrum–Effect Relationship by Q-Marker Selection

“…Long-term preservation of low phosphorylation levels of insulin receptors is one of the causes of insulin resistance [ 6 ]. In T2DM individuals, PTP1B is overexpressed [ 12 ], resulting in the insufficient phosphorylation of insulin receptors, thereby impairing the normal regulatory function of the insulin signaling pathway. The change in the biological activity of insulin in the tissue prompts an increase in insulin secretion by pancreas islet β-cells, counteracting the lack of insulin action and resulting in hyperinsulinemia.…”

Integrated widely targeted metabolomics and network pharmacology revealed quality disparities between Guizhou and conventional producing areas of Codonopsis Radix

“…It has been widely applied to the study of secondary metabolites in medicinal plants such as Dendrobium officinale Kimura et Migo ( 27 ), Pueraria lobata (Willd.) Ohwi ( 28 , 29 ), Curcuma Longa L. ( 30 ), Lycium barbarum L. ( 31 ), Scutellaria baicalensis Georgi ( 32 ), and Sesamum indicum L. ( 33 ). In contrast, studies on the metabolomics of CR have only been conducted on the traditional producing areas, while few studies have been reported on non-traditional producing areas such as Guizhou.…”