Widely targeted metabolomic analysis reveals dynamic changes in non-volatile and volatile metabolites during green tea processing

Wang, Huajie; Jin, Hua; Yu, Qinyan; Li, Jia; Wang, Jinjin; Deng, Yuliang; Yuan, Haibo; Jiang, Yongwen

doi:10.1016/j.foodchem.2021.130131

Cited by 130 publications

(93 citation statements)

References 24 publications

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“…However, the number of identified flavonoid components in ZBLs remains quite limited. Widely targeted metabolomics is a new method that has been widely applied in food research (17,20). To the best of our knowledge, the present study is the first widely targeted metabolomic analysis of ZBLs.…”

Section: Flavonoid Components In Zbls and The Effects Of Drought Stre...mentioning

confidence: 94%

“…The mobile phase was composed of ultrapure water with 0.1% formic acid (solvent A) and acetonitrile with 0.1% formic acid (solvent B). The gradient of solvent B was as follows: 0 min, 5%; 0-9 min, raised to 95%; 9-10 min, 95%; 10-11.10 min, reduced to 5%; 11.10-14 min, 5% (17). The column temperature was set to 40 • C, and the injection volume was 4 µL.…”

Section: Flavonoid Metabolite Profiling Of Zbls By Uplc-ms/msmentioning

confidence: 99%

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Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress

Hong

Fei

et al. 2022

Front. Nutr.

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Zanthoxylum bungeanum Maxim. leaves (ZBLs) are rich in flavonoids and have become popular in nutrition, foods and medicine. However, the flavonoid components in ZBLs and the mechanism of flavonoid biosynthesis under drought stress have received little attention. Here, we performed an integrative analysis of the metabolome and transcriptome of ZBLs from HJ (Z. bungeanum cv. “Hanjiao”) and FJ (Z. bungeanum cv. “Fengjiao”) at four drought stages. A total of 231 individual flavonoids divided into nine classes were identified and flavones and flavonols were considered the most abundant flavonoid components in ZBLs. The total flavonoid content of ZBLs was higher in FJ; it increased in FJ under drought stress but decreased in HJ. Nine-quadrant analysis identified five and eight differentially abundant flavonoids in FJ and HJ leaves, respectively, under drought stress. Weighted gene correlation network analysis (WGCNA) identified nine structural genes and eight transcription factor genes involved in the regulation of flavonoid biosynthesis. Moreover, qRT-PCR results verified the accuracy of the transcriptome data and the reliability of the candidate genes. Taken together, our results reveal the flavonoid components of ZBLs and document changes in flavonoid metabolism under drought stress, providing valuable information for nutrition value and food utilization of ZBLs.

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Section: Flavonoid Components In Zbls and The Effects Of Drought Stre...mentioning

confidence: 94%

Section: Flavonoid Metabolite Profiling Of Zbls By Uplc-ms/msmentioning

confidence: 99%

Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress

Hong

Fei

et al. 2022

Front. Nutr.

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“…Non-targeted metabolomics technology can qualitatively determine the metabolites based on existing databases, with high coverage of compounds, however, low accuracy. The key metabolites must be confirmed by standard products ( 14 ). Widely targeted metabolomics is a new technology that integrates the advantages of non-target and targeted metabolites detection technologies to achieve wide coverage, high throughput, and sensitivity ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Zhang

et al. 2021

Front. Nutr.

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Cistanche deserticola is one of the most precious plants, traditionally as Chinese medicine, and has recently been used in pharmaceutical and healthy food industries. Steaming and drying are two important steps in the processing of Cistanche deserticola. Unfortunately, a comprehensive understanding of the chemical composition changes of Cistanche deserticola during thermal processing is limited. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics analysis was used to investigate the transformation mechanism of Cistanche deserticola active compounds during steaming and drying processes. A total of 776 metabolites were identified in Cistanche deserticola during thermal processing, among which, 77 metabolites were differentially regulated (p < 0.05) wherein 39 were upregulated (UR) and 38 were downregulated (DR). Forty-seven (17 UR, 30 DR) and 30 (22 UR, 8 DR) differential metabolites were identified during steaming and drying, respectively. The most variation of the chemicals was observed during the process of steaming. Metabolic pathway analysis indicated that phenylpropanoid, flavonoid biosynthesis, and alanine metabolism were observed during steaming, while glycine, serine, and threonine metabolism, thiamine metabolism, and unsaturated fatty acid biosynthesis were observed during drying. The possible mechanisms of the chemical alterations during thermal processing were also provided by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Furthermore, the blackening of the appearance of Cistanche deserticola mainly occurred in the steaming stage rather than the drying stage, which is associated with the metabolism of the amino acids. All results indicated that the formation of active compounds during the processing of Cistanche deserticola mainly occurred in the steaming stage.

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“…Flavonoid glycosides, caffeine, and catechins influence the bitterness and organic acids impact on the fruitiness and acidity. Carotenoids and fat-soluble chlorophylls have an effect on the physical parameters, such as the color and shape of the dry tea [108]. The quality of post-harvest plant-based products is also related to the evaluation of the presence of contaminants, microorganisms, heavy metals, and maximum levels of permitted substances [25].…”

Section: Food Safetymentioning

confidence: 99%

Food Metabolites as Tools for Authentication, Processing, and Nutritive Value Assessment

Pedrosa

Lima

Heleno

et al. 2021

Foods

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Secondary metabolites are molecules with unlimited applications that have been gaining importance in various industries and studied from many angles. They are mainly used for their bioactive capabilities, but due to the improvement of sensibility in analytical chemistry, they are also used for authentication and as a quality control parameter for foods, further allowing to help avoid food adulteration and food fraud, as well as helping understand the nutritional value of foods. This manuscript covers the examples of secondary metabolites that have been used as qualitative and authentication molecules in foods, from production, through processing and along their shelf-life. Furthermore, perspectives of analytical chemistry and their contribution to metabolite detection and general perspectives of metabolomics are also discussed.

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Widely targeted metabolomic analysis reveals dynamic changes in non-volatile and volatile metabolites during green tea processing

Cited by 130 publications

References 24 publications

Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress

Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Food Metabolites as Tools for Authentication, Processing, and Nutritive Value Assessment

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