Widefield FT‐IR 2D and 3D Imaging at the Microscale Using Synchrotron Radiation

Mattson, Eric C.; Unger, Miriam; Sedlmair, Julia; Nasse, Michael; Aboualizadeh, Ebrahim; Alavi, Zahrasadat; Hirschmugl, Carol J.

doi:10.1002/9783527678136.ch15

Cited by 2 publications

(2 citation statements)

References 74 publications

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“…Precise rotation of the stage under the microscope was computer controlled. 36 The Murchison grain was rotated in increments of 1.6°. At each angle, an IR transmission spectrum was collected for each pixel in a 2D array detector simultaneously.…”

Section: Samples and Experimental Detailsmentioning

confidence: 99%

Synchrotron-Based Three-Dimensional Fourier-Transform Infrared Spectro-Microtomography of Murchison Meteorite Grain

et al. 2016

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We demonstrate nondestructive, three-dimensional, microscopic, infrared (IR) spectral in-situ imaging of an extraterrestrial sample. Spatially resolved chemical composition and spatial correlations are investigated within a single 45 µm grain of the Murchison meteorite. Qualitative and quantitative investigation through this analytical technique can help elucidate the origin and evolution of meteoritic compounds as well as parent body processes without damaging or altering the investigated samples.

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Section: Samples and Experimental Detailsmentioning

confidence: 99%

Synchrotron-Based Three-Dimensional Fourier-Transform Infrared Spectro-Microtomography of Murchison Meteorite Grain

et al. 2016

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“…Infrared (IR) microspectroscopy has been applied to biological systems for cell and tissue mapping, − for example, for probing the subcellular distribution of anticancer agents, characterization of adrenal gland tumors, probing H/D exchange in live cells, and analysis of hydration water around proteins . Here, we make use of synchrotron IR microspectroscopy to develop a method for addressing specific chemical changes at sites within redox proteins in response to electrochemically induced changes in oxidation state.…”

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confidence: 99%

Synchrotron-Based Infrared Microanalysis of Biological Redox Processes under Electrochemical Control

et al. 2016

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We describe a method for addressing redox enzymes adsorbed on a carbon electrode using synchrotron infrared microspectroscopy combined with protein film electrochemistry. Redox enzymes have high turnover frequencies, typically 10–1000 s–1, and therefore, fast experimental triggers are needed in order to study subturnover kinetics and identify the involvement of transient species important to their catalytic mechanism. In an electrochemical experiment, this equates to the use of microelectrodes to lower the electrochemical cell constant and enable changes in potential to be applied very rapidly. We use a biological cofactor, flavin mononucleotide, to demonstrate the power of synchrotron infrared microspectroscopy relative to conventional infrared methods and show that vibrational spectra with good signal-to-noise ratios can be collected for adsorbed species with low surface coverages on microelectrodes with a geometric area of 25 × 25 μm2. We then demonstrate the applicability of synchrotron infrared microspectroscopy to adsorbed proteins by reporting potential-induced changes in the flavin mononucleotide active site of a flavoenzyme. The method we describe will allow time-resolved spectroscopic studies of chemical and structural changes at redox sites within a variety of proteins under precise electrochemical control.

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Widefield FT‐IR 2D and 3D Imaging at the Microscale Using Synchrotron Radiation

Cited by 2 publications

References 74 publications

Synchrotron-Based Three-Dimensional Fourier-Transform Infrared Spectro-Microtomography of Murchison Meteorite Grain

Synchrotron-Based Three-Dimensional Fourier-Transform Infrared Spectro-Microtomography of Murchison Meteorite Grain

Synchrotron-Based Infrared Microanalysis of Biological Redox Processes under Electrochemical Control

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