Wide Range of Molecular Weight Distribution of Mitogenic Substance(s) in the Hot Water Extract of a Chinese Herbal Medicine, Bupleurum chinense.

Izumi, Shigeru; Ohno, Naohito; Kawakita, Tetsuya; Nomoto, Kikuo; Yadomae, Toshiro

doi:10.1248/bpb.20.759

Cited by 10 publications

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“…Meanwhile, in an analysis of the active components in bupleurum root, a crude drug, we found that polyphenols polymerized by enzymes have a strong immunoenhancing effect ( 53–55 ). A. brasiliensis also has a number of enzymes related to the polymerization of polyphenols ( 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers

Liu¹,

Fukuwatari²,

Okumura

et al. 2007

Evidence-Based Complementary and Alternative Medicine

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We performed studies on murine models and human volunteers to examine the immunoenhancing effects of the naturally outdoor-cultivated fruit body of Agaricus brasiliensis KA21 (i.e. Agaricus blazei). Antitumor, leukocyte-enhancing, hepatopathy-alleviating and endotoxin shock-alleviating effects were found in mice. In the human study, percentage body fat, percentage visceral fat, blood cholesterol level and blood glucose level were decreased, and natural killer cell activity was increased. Taken together, the results strongly suggest that the A. brasiliensis fruit body is useful as a health-promoting food.

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Section: Discussionmentioning

confidence: 99%

Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers

Liu¹,

Fukuwatari²,

Okumura

et al. 2007

Evidence-Based Complementary and Alternative Medicine

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“…families locally found in Taiwan. 3) Previous studies have shown that Bupleuri Radix possesses a wide range of immunopharmacologic functions, such as anti-inflammatory, mitogen-induced lymphocyte transformation, or antiviral activities. [4][5][6] A recent study reported that the acetone extract of Bupleurum scorzonerifolium could inhibit proliferation of A549 human lung cancer cells by inducing apoptosis and suppressing telomerase activity. 1) Apoptosis has been characterized as a fundamental cellular activity to maintain the physiological balance of the organism.…”

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confidence: 99%

The Antiproliferative Activity of Saponin-Enriched Fraction from Bupleurum Kaoi Is through Fas-Dependent Apoptotic Pathway in Human Non-small Cell Lung Cancer A549 Cells

Hsu

Kuo

Weng

et al. 2004

Biological & Pharmaceutical Bulletin

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Lung cancer is the leading cause of cancer death in the world, and non-small cell lung carcinoma (NSCLC) accounts for approximately 75-85% of these cancers. Non-small cell lung cancers commonly develop resistance to radiation and chemotherapy, and they often present at stages beyond surgical remedy. Since current treatment modalities are inadequate, novel therapies are necessary to reduce the effects of the increasing incidence in pulmonary neoplasm. 1,2)Bupleuri Radix (Chai-hu in Chinese and Saiko in Japanese) is one of the most important traditional Chinese crude drugs for treating hepatitis malaria and intermittent fever. It has been used in many Chinese medicine prescriptions to treat deafness, dizziness, irregular menstruation, lung disease, jaundice, liver disease and amenorrhea. Among them, B. kaoi is one of the Bupleurum spp. families locally found in Taiwan. 3) Previous studies have shown that Bupleuri Radix possesses a wide range of immunopharmacologic functions, such as anti-inflammatory, mitogen-induced lymphocyte transformation, or antiviral activities. [4][5][6] A recent study reported that the acetone extract of Bupleurum scorzonerifolium could inhibit proliferation of A549 human lung cancer cells by inducing apoptosis and suppressing telomerase activity. 1)Apoptosis has been characterized as a fundamental cellular activity to maintain the physiological balance of the organism. It is also involved in immune defense machinery and plays a necessary role as a protective mechanism against carcinogenesis by eliminating damaged cells or abnormal excess cells proliferated owing to various chemical agents' induction. 7) In this study, we determined the antiproliferative activity of SEF, and examined its effect on apoptosis in the human lung cancer cell line, A549. Furthermore, to establish the anticancer mechanism of SEF, we assayed the levels of Fas/ APO-1 receptor and Fas ligand which are strongly associated with the signal transduction pathway of apoptosis and affect the chemosensitivity of tumor cells to anticancer agents. MATERIALS AND METHODS MaterialsFetal bovine serum (FBS), penicillin G, streptomycin, and amphotericin B were obtained from GIBCO BRL (Gaithersburg, MD, U.S.A.). Dimethyl sulfoxide (DMSO) and RPMI-1640 were purchased from Sigma Chemical (St. Louis, MO, U.S.A.). Sodium 3Ј-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfoic acid hydrate (XTT) kit was obtained from Roche Diagnostics GmbH (Germany). Nucleosome ELISA, Fas Ligand, and Fas/APO-1 ELISA kits were purchased from Calbiochem (Cambridge, MA, U.S.A.).Preparation of B. kaoi Extracts Saponin-enriched fraction (SEF) was extracted from the roots of B. kaoi using the methods of Lin et al.3) SEF was dissolved in dimethyl sulfoxide (DMSO) and stored at Ϫ20°C. For all experiments, final concentrations of the tested compound were prepared by diluting the stock with RPMI-1640. Control cultures received the carrier solvent (0.1% DMSO).Cell Culture A549 (American Type Culture Collection [ATCC] CCL185) was maintaine...

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Bupleurum kaoi inhibits Coxsackie B virus type 1 infection of CCFS-1 cells by induction of type I interferons expression

Cheng

Chiang

Yen

et al. 2007

Food and Chemical Toxicology

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Wide Range of Molecular Weight Distribution of Mitogenic Substance(s) in the Hot Water Extract of a Chinese Herbal Medicine, Bupleurum chinense.

Cited by 10 publications

References 0 publications

Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers

Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers

The Antiproliferative Activity of Saponin-Enriched Fraction from Bupleurum Kaoi Is through Fas-Dependent Apoptotic Pathway in Human Non-small Cell Lung Cancer A549 Cells

Bupleurum kaoi inhibits Coxsackie B virus type 1 infection of CCFS-1 cells by induction of type I interferons expression

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