Wide Prevalence of Critically Important Antibiotic Resistance in Egyptian Quail Farms with Mixed Infections

Farghal, Eman M.; Samy, Ahmed; Roshdy, Heba

doi:10.17582/journal.vsrr/2017.3.1.17.24

Cited by 4 publications

(4 citation statements)

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“…The occurrence of E. coli was obtained as 100 per cent in the present study whereas 76.66 per cent of samples were reported as positive for the presence of E. coli by Sekhar et al, (2017) and a much lower isolation rate of 6 per cent was documented by Farghaly et al, (2017). The higher rate of occurrence of E. coli in the present study may be due to a lack of implementation of a proper biosecurity plan on farms or due to the variation in the factors such as geographical location, sample size, season and techniques of sampling and isolation.…”

Section: Occurrence Of E Coli and Salmonella Spp In Japanese Quailcontrasting

confidence: 58%

Phenotypic and Genotypic Characterisation of ESBL Producing and Carbapenem-resistant Escherichia coli and Salmonella spp. in Japanese Quail Farms of Wayanad District

Andrews¹,

Asha²,

Vergis³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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The long-term indiscriminate usage of antimicrobials in poultry for growth promotion and therapeutic purposes has resulted in the emergence of multi-drug resistant (MDR) bacteria like E. coli and Salmonella spp. The food-producing birds harbouring MDR genes of ESBL and Carbapenemases may possess health risks to the human population and is a major pressing issue affecting the market for broiler meat. The present study was conducted to find the occurrence of E. coli and Salmonella spp. in broiler Japanese quails of the Wayanad district and to detect the presence of ESBL and carbapenem resistance. A total of 22 cloacal swabs, two feed and two water samples were analysed for E. coli and Salmonella spp. by conventional microbiological, biochemical and molecular methods and the occurrence was observed as 100 and 9.09 per cent, respectively. The phenotypic characterization of isolates for ESBL production and carbapenem resistance was tested through disc diffusion method as per CLSI 2019 guidelines. The isolates were screened for blaCTX-M, blaSHV and blaTEM genes for ESBL genotypic characterization and 90.90 per cent of E. coli isolates were found to harbour at least one ESBL gene of interest whereas, negative results were obtained for Salmonella spp. isolates in both phenotypic and genotypic assay. None of the isolates was found positive for the presence of carbapenem-resistant S genes blaNDM, blaOXA-48, blaIMP and blaVIM. Thus, a diverse phenotypic and genotypic pattern of ESBL and carbapenem resistance was observed among isolates.

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Section: Occurrence Of E Coli and Salmonella Spp In Japanese Quailcontrasting

confidence: 58%

Phenotypic and Genotypic Characterisation of ESBL Producing and Carbapenem-resistant Escherichia coli and Salmonella spp. in Japanese Quail Farms of Wayanad District

Andrews¹,

Asha²,

Vergis³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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“…Resistensi terhadap antibiotik terhadap ExPEC isolat puyuh telah dilaporkan terjadi pada 1993, diantaranya Ampisilin, Amikasin, Klorapfenikol. Gentamisin, Neomisin, Karbamisin dan Sefektasim (Saha et al, 1993) dan resisten terhadap Doksisiklin, Tetrasiklin dan Trimetoprim dan Kloramfenikol (Farghaly et al, 2017).…”

Section: Hasil Dan Pembahasanunclassified

Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica)

Prihtiyantoro,

Purnomo,

et al. 2023

Jurnal Sain Veteriner

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Extraintestinal Escherichia coli (ExPEC) causes colibacillosis in poultry, including quail. ExPEC is a pathogenic E. coli that causes colibacillosis outside the gastrointestinal tract of poultry, in the form of peritonitis, pericarditis, salpingitis, synovitis, osteomyelitis, septicemia, pneumonitis, nephritis, pleurisy, proventriculitis and ventriculitis. In poultry farming antibiotics are used to promote growth and egg production, prevention and treatment of bacterial infections. Antibiotic resistance is a new problem that arises in poultry farming. In this study, 24 isolates of ExPEC strain from quail were used. These isolates did not differentiate sorbitol in the MacConkey (SMAC) sorbitol test assay. Antibiotic resistance was tested based on the inhibition of bacterial growth on Muller Hinnton Agar (MHA) media. 10 types of antibiotics were used to observe their resistance in all isolates. Resistance to Amikacin (87.5%), Ampicillin (87.5%), Ciprofloxacin (100%), Clindamycin (95.83%), Cefoxacin (25.0%), Doxycycline (87.5%), Erythromycin (100%), Gentamicin (95.83%), Penicillin (95.83%) and Tetracycline (83.3%). The isolates had multidrug-resistance between 3 to 10 types of antibiotics. This study showed that ExPEC isolates from quail were resistant to the type of antibiotics tested, so that the use of antibiotics in quail culture should be limited to reduce and prevent the emergence of new antibiotic resistance. Keyword: Escherichia coli, sorbitol-negative, quail, antibiotics resistance

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“…P.aeruginosa is a profiteer pathogen leads to respiratory infections, septicemia and high mortality of embryos and newly hatched chicks (Dinev et al 2013Farghal et al 2017). Infection of yolk sac by pseudomonas is enhanced by its ability to degrade of other pathogen (Elsayed et al 2016).…”

Section: Bakheet Et Al 2017)mentioning

confidence: 99%

The role of hatcheries, hatching eggs and one day old chicks in dissemination of beta lactam antibiotic resistance Pseudomonas aeruginosa (ESBL)

2021

Egyptian Journal of Animal Health

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O ut of 450 samples collected from infertile egg ,dead in shell embryo ,baby chicks with omphalitis and hatcheries environment P. aeruginosa was identified in 12. 22% (55/450) of the total examined samples. Two strains (4%) were isolated from infertile egg,18 strains (12%) from dead in shell embryo ,33 strain(15%) from baby chicks and 2 strains (6.7%%) from hatcheries environment. Extended spectrum beta lactam ( ESBL) production was confirmed in 8 isolates(14.55%) by double disk diffusion test . blaSHV and blaTEM genes were detected in (8/8)and (7/8) of ESBL-P. aeruginosa isolates respectively, whereas none of these isolates harbored, blaOXA and blaCTX-M genes. Molecular detection of (oprL) and (toxA) genes were amplified in in all eight studied strains. The results of antibiotic sensitivity for ESBL producing P. aeruginosa isolates indicated that the isolates were susceptible to norfloxacin, ciprofloxacin and colistin sulphate .On the other hand, all isolates were completely resistant to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazol and most of the isolates were resistant to streptomycin and tetracycline. The pathogenicity test of the isolated field strain of ESBL-P. aeruginosa proved to be highly pathogenic induced mortality rate of 43.33% . The predominant lesions of dead and sacrificed chicks were septicemia, congestion of internal organs (liver, heart& lung) and inflamed intestine. Histopathological findings supported the previous gross lesions. In conclusion, it can be said that there is an upsurge of beta lactamase producing Pseudomonas aeruginosa infer that one day old chicks and hatcheries can serves as a reservoir for growth and dissemination of clinically significant antibiotic resistance among bacterial species.

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Wide Prevalence of Critically Important Antibiotic Resistance in Egyptian Quail Farms with Mixed Infections

Cited by 4 publications

References 0 publications

Phenotypic and Genotypic Characterisation of ESBL Producing and Carbapenem-resistant Escherichia coli and Salmonella spp. in Japanese Quail Farms of Wayanad District

Phenotypic and Genotypic Characterisation of ESBL Producing and Carbapenem-resistant Escherichia coli and Salmonella spp. in Japanese Quail Farms of Wayanad District

Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica)

The role of hatcheries, hatching eggs and one day old chicks in dissemination of beta lactam antibiotic resistance Pseudomonas aeruginosa (ESBL)

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