Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmid

Knauf, Vic; Nester, Eugene W.

doi:10.1016/0147-619x(82)90040-3

Cited by 566 publications

(262 citation statements)

References 21 publications

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“…Virus DNA, purified as described previously (Smith & Crook, 1988 b), was partially digested with SalI and ligated into SalI-cut pVK102 (Knauf & Nester, 1982). Ligated DNA was added to a λ packaging mix (Promega) and used to transform E. coli HB101 cells.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region.

Crook¹,

James²,

Smith³

et al. 1997

Journal of General Virology

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A cloned strain of Cydia pomonella granulovirus, CpGV-M1, was obtained using successive rounds of an in vivo limiting dilution method. A detailed physical map of the genome was constructed using 11 restriction enzymes. The region containing the granulin gene and an open reading frame immediately upstream of the granulin gene was sequenced. This region showed a high degree of homology to the equivalent region from Cryptophlebia leucotreta granulovirus with 98 % amino acid identity for the granulins and 68 % identity for the putative polypeptides encoded by the upstream ORFs. These

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Section: Methodsmentioning

confidence: 99%

Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region.

Crook¹,

James²,

Smith³

et al. 1997

Journal of General Virology

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“…Sci. USA 88 (1991) strain DE3-la was constructed in cosmid pVK102 as described (26). Cosmid clones corresponding to the GSN and nod gene regions were identified by hybridization to the HindIII fragments of pMJS12 and pMJS18 and mated into B. japonicum strain SD6-lc as described above.…”

Section: Methodsmentioning

confidence: 99%

“…The HindIll fragments of cosmid pR32 were subcloned in pVK102 as described (25,26). Cosmids containing fragments corresponding to the nodDl YABC, nodSUIJ, nodZ, nodD2, or other gene regions (7-9, 25, 27) were used to transform E. coli strain S17-1 (ref.…”

Section: Methodsmentioning

confidence: 99%

The Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeans.

Sadowsky

Cregan

Göttfert

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

139

115

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Several soybean genotypes have been identified which specifically exclude modulation by members of Bradyrhizobiumjaponicum serocluster 123. We have identified and sequenced a DNA region from B. japonicum strain USDA 110 which is involved in genotype-specifc modulation of soybeans. This 2.3-kilobase region, cloned in pMJS12, allows B. japonicum serocluster 123 isolates to form nodules on plants of serogroup 123-restricting genotypes. The nodules, however, were ineffective for symbiotic nitrogen fixation. The nodulation-complementing region is located approximately 590 base pairs transcriptionally downstream from nodD2. The 5' end of pMJS12 contains a putative open reading frame (ORF) of 710 base pairs, termed nowl. Transposon Tn3-HoHo mutations only within the ORF abolished modulation complementation. The N terminus of the predicted noL4 gene product has strong similarity with the N terminus of MerR, the regulator of mercury resistance genes. Translational IacZ fusion experiments indicated that nolA was moderately induced by soybean seed extract and the isoflavone genistein. Restriction fragments that hybridize to pMJS12 were detected in genomic DNAs from both nodulation-restricted and -unrestricted strains.

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“…Thus far, only large (19-23 kb) IncP vectors with limited cloning sites available have been used with success in M. extorquens AM1. These include the cloning vectors pRK310 (Ditta et al, 1985) and pVK100 (Knauf & Nester, 1982), and the promoter-probe vectors pHX200 (Xu et al, 1993) and pGD500 (Ditta et al, 1985). No IncQ vectors or smaller IncP vectors have been found to be maintained in this strain (Lidstrom, 1992).…”

Section: Abbreviationsmentioning

confidence: 99%

Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720.

2001

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Full exploitation of the information available in bacterial genome sequences requires the availability of facile tools for rapid genetic manipulation. One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1. IncQ and small IncP vectors were shown to be unsuitable for use in this bacterium, but a spontaneous mutant of a small IncP plasmid was isolated that functioned efficiently in M. extorquens AM1. This plasmid was sequenced and used as a base for developing improved broad-host-range cloning vectors. These vectors were found to replicate in a wide variety of bacterial species and have the following advantages : (1) high copy number in Escherichia coli ; (2) small size (72 and 80 kb) ; (3) complete sequences ; (4) variety of unique restriction sites ; (5) blue-white screening via lacZα ; (6) conjugative mobilization between bacterial species ; and (7) readily adaptable into species-specific promoter-probe and expression vectors. Two low-background promoter-probe vectors were constructed based on these cloning vectors with either lacZ or xylE as reporter genes ; these were shown to report gene expression effectively in M. extorquens AM1. Specific expression vectors were developed for use in M. extorquens AM1, which were shown to express foreign genes at significant levels, and a simple strategy is outlined to develop specific expression vectors for other bacteria. The strong mxaF promoter was used for expression, since E. coli lac-derived promoters were expressed at very low levels. This suite of genetic tools will enable a more sophisticated analysis of the physiology of M. extorquens AM1, and these vectors should also be valuable tools in the study of a variety of bacterial species.

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Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmid

Cited by 566 publications

References 21 publications

Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region.

Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region.

The Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeans.

Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria The GenBank accession numbers for the sequences reported in this paper are AF327711, AF327712, AF327713, AF327714, AF327715, AF327716, AF327717, AF327718, AF327719 and AF327720.

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