Wide genetic heterogeneity and low antimicrobial resistance of enteroaggregative Escherichia coli isolates from several rural communities

Guerrieri, Caroline Gastaldi; Monfardini, Mariane Vedovatti; Silva, Eliza Andrade; Freitas, Luciana Bueno de; Schuenck, Ricardo Pinto; Spano, Liliana Cruz

doi:10.1099/jmm.0.001120

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…Several of the virulence factor-encoding genes investigated here were statistically more frequent among typical EAEC, which is not surprising since the majority of these genes were identified, and functionally characterized, in the typical EAEC prototype strain 042 (Harrington et al, 2006;Chaudhuri et al, 2010;Hebbelstrup Jensen et al, 2014). Similar findings were also observed by other Brazilian authors that compared typical and atypical EAEC isolates, especially with the pic, aap, shf, and agg4A genes being more frequent among the typical EAEC isolates (Andrade et al, 2017;Taborda et al, 2018;Guerrieri et al, 2019). Case-control studies, performed in distinct geographic regions, have been able to demonstrate the association of some genes encoding virulence factors with diarrheal disease.…”

Section: Discussionsupporting

confidence: 89%

“…In typical EAEC the establishment of the AA pattern is mediated by five distinct AAFs (Jønsson et al, 2017a), and the occurrence of genes associated with the biogenesis of AAF/I-AAF/IV has already been reported among Brazilian EAEC isolates, with their frequency varying according to the geographic regions studied (Elias et al, 1999;Zamboni et al, 2004;Lima et al, 2013;Guerrieri et al, 2019). However, the occurrence of Brazilian EAEC isolates harboring the agg5A, alone or concomitantly with agg3A, is described for the first time in this study.…”

Section: Discussionmentioning

confidence: 48%

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Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil

Dias

Tanabe

Vieira

et al. 2020

Front. Cell. Infect. Microbiol.

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Enteroaggregative Escherichia coli (EAEC) is an important agent of acute and persistent diarrhea in children and adults worldwide. Here we report a characterization of 220 EAEC isolates, 88.2% (194/220) of which were typical and 11.8% (26/220) were atypical, obtained from diarrheal patients during seven years (2010)(2011)(2012)(2013)(2014)(2015)(2016) of epidemiological surveillance in Brazil. The majority of the isolates were assigned to phylogroups A (44.1%, 97/220) or B1 (21.4%, 47/220). The aggregative adherence (AA) pattern was detected in 92.7% (204/220) of the isolates, with six of them exhibiting AA concomitantly with a chain-like adherence pattern; and agg5A and agg4A were the most common adhesin-encoding genes, which were equally detected in 14.5% (32/220) of the isolates. Each of 12 virulence factor-encoding genes (agg4A, agg5A, pic, aap, aaiA, aaiC, aaiG, orf3, aar, air, capU, and shf) were statistically associated with typical EAEC (P < 0.05). The genes encoding the newly described aggregate-forming pili (AFP) searched (afpB, afpD, afpP, and afpA2), and/or its regulator (afpR), were exclusively detected in atypical EAEC (57.7%, 15/26), and showed a significant association with this subgroup of EAEC (P < 0.001). In conclusion, we presented an extensive characterization of the EAEC circulating in the Brazilian settings and identified the afp genes as putative markers for increasing the efficiency of atypical EAEC diagnosis.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 48%

Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil

Dias

Tanabe

Vieira

et al. 2020

Front. Cell. Infect. Microbiol.

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“…The dispersin encoding gene is highly prevalent in EAEC collections of different studies, yet not present in all strains (Czeczulin et al, 1999;Elias et al, 2002;Jenkins et al, 2007;Boisen et al, 2012;Durand et al, 2016;Havt et al, 2017;Hebbelstrup Jensen et al, 2017;Dias et al, 2020;Guerrieri et al, 2020). The presence of aap was also detected in EAEC strains capable to cause urinary tract infection (Olesen et al, 2012;Boll et al, 2013;Herzog et al, 2014) and in Shiga toxin-producing EAEC of serotypes O104:H4 and 0111:H21 (Scheutz et al, 2011;Dallman et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Surface Protein Dispersin of Enteroaggregative Escherichia coli Binds Plasminogen That Is Converted Into Active Plasmin

et al. 2020

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Dispersin is a 10.2 kDa-immunogenic protein secreted by enteroaggregative Escherichia coli (EAEC). In the prototypical EAEC strain 042, dispersin is non-covalently bound to the outer membrane, assisting dispersion across the intestinal mucosa by overcoming electrostatic attraction between the AAF/II fimbriae and the bacterial surface. Also, dispersin facilitates penetration of the intestinal mucus layer. Initially characterized in EAEC, dispersin has been detected in other E. coli pathotypes, including those isolated from extraintestinal sites. In this study we investigated the binding capacity of purified dispersin to extracellular matrix (ECM), since dispersin is exposed on the bacterial surface and is involved in intestinal colonization. Binding to plasminogen was also investigated due to the presence of conserved carboxy-terminal lysine residues in dispersin sequences, which are involved in plasminogen binding in several bacterial proteins. Moreover, some E. coli components can interact with this host protease, as well as with tissue plasminogen activator, leading to plasmin production. Recombinant dispersin was produced and used in binding assays with ECM molecules and coagulation cascade compounds. Purified dispersin bound specifically to laminin and plasminogen. Interaction with plasminogen occurred in a dose-dependent and saturable manner. In the presence of plasminogen activator, bound plasminogen was converted into plasmin, its active form, leading to fibrinogen and vitronectin cleavage. A collection of E. coli strains isolated from human bacteremia was screened for the presence of aap, the dispersin-encoding gene. Eight aap-positive strains were detected and dispersin production could be observed in four of them. Our data describe new attributes for dispersin and points out to possible roles in mechanisms of tissue adhesion and dissemination, considering the binding capacity to laminin, and the generation of dispersin-bound plasmin(ogen), which may facilitate E. coli spread from the colonization site to other tissues and organs. The cleavage of fibrinogen in the bloodstream, may also contribute to the pathogenesis of sepsis caused by dispersin-producing E. coli.

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Microorganisms responsible for neonatal diarrhea

Lucero,

Velarde,

O’Ryan

2025

Remington and Klein's Infectious Diseases of the Fetus and Newborn Infant

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Wide genetic heterogeneity and low antimicrobial resistance of enteroaggregative Escherichia coli isolates from several rural communities

Cited by 4 publications

References 47 publications

Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil

Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil

Surface Protein Dispersin of Enteroaggregative Escherichia coli Binds Plasminogen That Is Converted Into Active Plasmin

Microorganisms responsible for neonatal diarrhea

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