Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues

Abstract

Targeted and localized mass spectrometry imaging allows faster characterization of cancer compared to conventional methods.

A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

“…Air flow‐assisted desorption electrospray ionization mass spectrometry imaging (AFADESI‐MSI) was developed by our group and applied in whole body molecular imaging, drug molecular mechanism study, and in situ biomarker discovery 8. For now, ambient ionization MSI has been widely used in histologic diagnosis of cancer and other diseases mainly based on lipid profiles 9. Several MSI studies have been reported to visualize small molecule metabolites in biosamples 10.…”

Mueller polarimetric imaging for surgical and diagnostic applications: a review

“…A number of applications of Mueller polarimetric imaging are instead based on detecting unstained thin tissue cuts [22,131,144,[158][159][160][161][162] in a transmission geometry. Since Haematoxylin and Eosin (H&E) staining and other staining techniques require time and elaborate sample preparation by a well-trained and experienced pathologist for diagnosis, unstained Mueller microscopy may be used for rapid and low cost pathological inspection [159,160] and instant tissue characterization [136,163].…”

“…Mass spectrometry is able to give quantitative pathological classification but suffers from long acquisition time if used to generate an image. Polarimetric imaging, especially depolarization imaging was demonstrated to be helpful to improve scanning efficiency and significantly shorten the diagnostic time [22]. In Figure 2(c), a pathological slide of a liver sample with fibrosis was imaged by polarimetric microscopy.…”

“…Mueller matrix descriptions have been extended towards tissue autofluorescence spectroscopy and initially explored for cervical cancerous tissue diagnosis, as shown in Figure 2(e). The additional linear and cir- [12,21,22,164,167] cular diattenuation spectra possess potential advantages over elastic scattering based approaches. Contrast can be observed between normal and precancerous groups attributed to the loss of anisotropic organization of collagen, which suggests that fluorescence diattenuation and polarizance originating from anisotropic organization of collagen in the connective tissue may serve as potentially useful diagnostic metrics [167].…”

“…The characteristic polarization information of interest is carried by a 4 3 4 matrix named a Mueller matrix and this can be experimentally obtained from Mueller polarimetry and polarimetric imaging. In recent years, an increasing number of biomedical applications of Mueller polarimetric imaging techniques have been proposed [5], e. g. oral cancerous legion detection [13], cervical cancer and colon cancer detection and staging [12,[14][15][16][17][18][19], assessment of cancer therapy [20], partial bladder obstruction diagnosis [21], guiding mass spectrometry for instant pathology [22], characterizing collagen fibres [23], and these have demonstrated advantages over polarization blind imaging.…”