Wide-field optical coherence tomography: imaging of biological tissues

Bordenave, E.; Abraham, Emmanuel; Jonusauskas, Gediminas; Tsurumachi, Noriaki; Oberlé, J.; Rullière, C.; Minot, P. E.; Lassègues, Maguy; Bazeille, J. E. Surlève

doi:10.1364/ao.41.002059

Cited by 63 publications

(23 citation statements)

References 13 publications

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“…Several modes were used such as heterodyne detection [26], wide-field configuration [27], phase-shift technique [28] and a stroboscopic mode [29]. Applications vary from imaging of the eye [30] to other scattering tissue [31][32][33]. Full-field OCT (FF-OCT) uses low spatial and temporal coherence similar to the well-known coherence probe microscopy (CPM) that have been in use for long time in optical metrology [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal coherence effects in interference microscopy and full‐field optical coherence tomography

Abdulhalim

2012

Annalen der Physik

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Low‐coherence optical microscopy or optical coherence microscopy uses light with short coherence length. The well‐known case is: “white‐light interferometry”, which became recently more known as: “optical coherence tomography”. However, when lenses and microscope objectives are used to create interferometric images, in what is known classically as “interference microscopy” or today as “full‐field optical coherence tomography” the spatial coherence starts to play a critical role. In this article the coherence effects in low‐coherence optical microscopy are reviewed. As this technology is becoming increasingly publicized due to its importance in three‐dimensional imaging, particularly of scattering biological media and optical metrology, the understanding of the fundamental physics behind it is essential. The interplay between longitudinal spatial coherence and temporal coherence and the effects associated with them are discussed in detail particularly when high numerical apertures are used. An important conclusion of this study is that a high‐contrast, high‐resolution system for imaging of multilayered samples is the one that uses narrowband illumination and high‐NA objectives with an index‐matching fluid. Such a system, when combined with frequency‐domain operation, can reveal nearly real‐time three‐dimensional images, and is thus competitive with confocal microscopy.

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Section: Introductionmentioning

confidence: 99%

Spatial and temporal coherence effects in interference microscopy and full‐field optical coherence tomography

Abdulhalim

2012

Annalen der Physik

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“…The axial resolution ∆z is determined by the coherence length ∆L, which is inversely proportional to the bandwidth of the light source (∆λ), as shown in following [10] :…”

Section: Methodsmentioning

confidence: 99%

Three dimensional image reconstruction based on a wide-field optical coherence tomography system

Feng

Zhang

et al. 2014

SPIE Proceedings

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Wide-field optical coherence tomography has a promising application for its high scanning rate and resolution. The principle of a wide-field optical coherence tomography system is described, and 2D images of glass slides are reconstructed using eight-stepped phase-shifting method in the system. Using VC6.0 and OpenGL programming, 3D images are reconstructed based on the Marching Cube algorithm with 2D image sequences. The experimental results show that the depth detection and three-dimensional tomography for translucent materials could be implemented efficiently in the WFOCT system.

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“…Point-by-point longitudinal [4,5] and en face scanning [6,7] has been used. Camera coupled OCT systems, which take images that are perpendicular to the microscope optical [8][9][10], have also been developed use spatially coherent illumination. Higher resolutions are achieved in these systems as 1604 The European Physical Journal Special Topics Fig.…”

Section: Introduction: Oct and Ffoct Backgroundmentioning

confidence: 99%

A resolution insensitive to geometrical aberrations by using incoherent illumination and interference imaging

Xiao

Fink

Gandjbakhche

et al. 2017

Eur. Phys. J. Spec. Top.

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Abstract. This contribution is another opportunity to acknowledge the influence of Roger Maynard on our research work when he pushed one of us (ACB) to explore the field of waves propagating in complex media rather than limiting ourselves to the wavelength scale of thermal waves or near field phenomena.Optical tomography is used for imaging in-depth scattering media such as biological tissues. Optical coherence tomography (OCT) plays an important role in imaging biological samples. Coupling OCT with adaptive optics (AO) in order to correct eye aberrations has led to cellular imaging of the retina. By using our approach called Full-Field OCT (FFOCT) we show that, with spatially incoherent illumination, the width of the point-spread function (PSF) that governs the resolution is not affected by aberrations that induce only a reduction of the signal level. We will describe our approach by starting with the PSF experimental data followed by a simple theoretical analysis, and numerical calculations. Finally full images obtained through or inside scattering and aberrating media will be shown.

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Wide-field optical coherence tomography: imaging of biological tissues

Cited by 63 publications

References 13 publications

Spatial and temporal coherence effects in interference microscopy and full‐field optical coherence tomography

Spatial and temporal coherence effects in interference microscopy and full‐field optical coherence tomography

Three dimensional image reconstruction based on a wide-field optical coherence tomography system

A resolution insensitive to geometrical aberrations by using incoherent illumination and interference imaging

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