Whole β-glucan particle attenuates AOM/DSS-induced colorectal tumorigenesis in mice via inhibition of intestinal inflammation

Abstract

Yeast β-glucan is a polysaccharide purified from the Saccharomyces cerevisiae cell wall, and its multiple biological activities are essential for immune regulation. However, the effect of β-glucan on the intestinal immune response during colitis-associated colorectal cancer (CAC) is unclear. Here, we explore the possible role of β-glucan in the development of CAC. Wild type (WT) mice with CAC induced by azoxmethane (AOM) and dextran sodium sulfate (DSS) had fewer tumors than untreated mice after oral β-glucan because of increased antitumor dendritic cells (DCs) in the tumor microenvironment, resulting in more CD8+ T cells and the production of related cytokines. β-glucan also increased resistance to DSS-induced chronic colitis by reshaping the inflammatory microenvironment. These data suggest that β-glucan improves experimental intestinal inflammation and delays the development of CAC. Therefore, β-glucan is feasible for treating chronic colitis and CAC in clinical practice.

Broccoli Cultivated with Deep Sea Water Mineral Fertilizer Enhances Anti-Cancer and Anti-Inflammatory Effects of AOM/DSS-Induced Colorectal Cancer in C57BL/6N Mice

“…Histopathology is a common method for assessing intestinal inflammation and pathology in animal models, typically revealing moderate to severe dysplasia in the epithelium of mouse tissues treated with AOM/DSS. This is accompanied by irregular crypt arrangement, clear infiltration of inflammatory cells in the mucosa, and the formation of typical tumors in the distal colon [33]. In this histopathological analysis, epithelial cell loss, crypt loss, and adenocarcinoma occurred in the control group; however, these symptoms were alleviated in the broccoli-treated group, especially the NFB group, showing similar features to the normal group.…”

“…Owing to the oral administration of broccoli extract to mice with AOM/DSS-induced CRC, it was confirmed that the colon lengthened, the colon weight/length ratio reduced, and the number of tumors reduced. DSS dissolved in drinking water is toxic to the epithelial lining of the large intestine, causing severe colitis, which is characterized by weight loss and bloody stool after DSS ingestion, and causes inflammation in the liver and spleen, causing weight gain [32,33]. Accordingly, in this study, the weight of the liver and spleen of the CON group increased significantly compared with that of the NOR group and was decreased in the groups that consumed broccoli extract.…”

β-glucan: a potent adjuvant in immunotherapy for digestive tract tumors

“…Additionally, β-glucan enhanced resilience against chronic colitis by remodeling the inflammatory microenvironment. These data suggest that β-glucan can ameliorate experimental intestinal inflammation and delay the progression of CAC ( 80 ). Ji Hyeon Kim and colleagues evaluated the therapeutic potential of LMW-AP-FBGin BALB/c mice implanted with subcutaneous CT-26 colon cancer cells.…”