Whole transcriptome-based miRNA-mRNA network analysis revealed the mechanism of inflammation-immunosuppressive damage caused by cadmium in common carp spleens

Teng, Xiaohua; Zhang, Shuai; Tong, Jianyu; Teng, Xiaohua; Zhang, Zhongyuan; Li, Shu

doi:10.1016/j.scitotenv.2020.137081

Cited by 81 publications

(21 citation statements)

References 55 publications

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“…The animal test lasted for 10 days, and the mice were ultimately sacrificed by cervical dislocation on the last day. The intestinal tissues were immediately transferred to a formaldehyde solution to observe morphological changes 14 . The protocols used in this experiment were approved by the Northeast Agricultural University Institutional Animal Care and Use Committee, and the ethical treatment of the animals used in this study was approved by the Animal Welfare Committee protocol (#NEAU‐[2013]‐9) at the Northeast Agricultural University (Harbin, China).…”

Section: Methodsmentioning

confidence: 99%

Oleanolic acid reshapes the gut microbiota and alters immune‐related gene expression of intestinal epithelial cells

Xue

et al. 2021

J Sci Food Agric

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BACKGROUND Oleanolic acid (OA) is a pentacyclic triterpenoid compound that is present at high levels in olive oil and has several promising pharmacological effects, such as liver protection and anti‐inflammatory, antioxidant, and anticancer effects. The purpose of the present study was to assess whether OA treatment affects gut health compared to a control condition, including gut microbiota and intestinal epithelial immunity. RESULTS Illumina MiSeq sequencing (16S rRNA gene) was used to investigate the effect of OA on the microbial community of the intestinal tract, while Illumina HiSeq (RNA‐seq) technology was used to investigate the regulatory effect of OA on gene expression in intestinal epithelial cells, which allowed for a comprehensive analysis of the effects of OA on intestinal health. The results showed that the consumption of OA initially controlled weight gain in mice and altered the composition of the gut microbiota. At the phylum level, OA significantly increased the relative abundances of cecum Firmicutes but decreased the abundance of Actinobacteria, and at the genus level it increased the relative abundance of potentially beneficial bacteria such as Oscillibacter and Ruminiclostridium 9. Oleanolic acid treatment also altered the expression of 12 genes involved in the Kyoto Encyclopedia of Genes and Genomes（KEGG）pathways of complement and coagulation cascades, hematopoietic cell lineage, and leukocyte transendothelial migration in intestinal epithelial cells to improve gut immunity. CONCLUSION Intake of OA can contribute beneficial effects by optimizing gut microbiota and altering the immune function of intestinal epithelial cells, potentially to improve intestinal health status. © 2021 Society of Chemical Industry.

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Section: Methodsmentioning

confidence: 99%

Oleanolic acid reshapes the gut microbiota and alters immune‐related gene expression of intestinal epithelial cells

Xue

et al. 2021

J Sci Food Agric

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“…Studies have demonstrated that miRNAs and lncRNAs can regulate the cell cycle, apoptosis, DNA damage, and inflammation in the mechanisms underlying Cd toxicity ( 78 – 81 ). Partial DElncRNAs and DEmiRNAs were selected for further analyses.…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Profiling of the Effects of Cadmium on the Liver of the Xiangxi Yellow Heifer

Wei

Yi²,

Shen

et al. 2022

Front. Vet. Sci.

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Cadmium (Cd) is a major heavy metal toxicant found in industrial zones. Humans and animals are exposed to it through their diet, which results in various physiological problems. In the current study, the toxic effects of Cd on the liver were investigated by whole-transcriptome sequencing (RNA-seq) of the livers of Xiangxi heifers fed a diet with excess Cd. We randomly divided six healthy heifers into two groups. The first group received a control diet, whereas the second group received Cd-exceeding diets for 100 days. After 100 days, the livers were collected. A total of 551 differentially expressed mRNAs, 24 differentially expressed miRNAs, and 169 differentially expressed lncRNAs were identified (p < 0.05, |log2FC| >1). Differentially expressed genes (DEGs) were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. We found that under Cd exposure, DEGs were enriched in the adenosine 5'-monophosphate–activated protein kinase pathway, which is involved in autophagy regulation, and the peroxisome proliferator–activated receptor pathway, which is involved in lipid metabolism. In addition, the apolipoprotein A4 gene, which has anti-inflammatory and antioxidant effects, the anti-apoptotic gene ATPase H+/K+ transporting the nongastric alpha2 subunit, and the cholesterol metabolism–associated gene endothelial lipase gene were significantly downregulated. C–X–C motif chemokine ligand 3, cholesterol 7α-hydroxylase, and stearoyl-CoA desaturase, which are involved in the development of fatty liver, were significantly upregulated. These genes revealed the main effects of Cd on the liver of Xiangxi yellow heifers. The current study provides insightful information regarding the DEGs involved in autophagy regulation, apoptosis, lipid metabolism, anti-inflammation, and antioxidant enzyme activity. These may serve as useful biomarkers for predicting and treating Cd-related diseases in the future.

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“…In fish, much literature related to miR-122 is available certifying the role in the immune [91,92] and in the metabolic systems being highly abundant in the zebrafish liver [93]. The presence of miR-122 was detailed in many fish species such as tilapia, medaka, carp, and in many fish tissues such as the spleen, head kidney [94][95][96][97].…”

Section: Discussionmentioning

confidence: 99%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2021

Preprint

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MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in a wide variety of physiological processes, including those related to the reproductive system. Although in the last decade a plethora of miRNAs has been reported, the miRNA alterations occurred by environmental cues and their biological functions have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation (18-32 days post fertilization, dpf), a treatment that results in male-skewed sex ratios. Once the fish reached adulthood (90 dpf), ovaries and testes were sequenced by high-throughput technologies. About 101 million high-quality reads were obtained from gonadal samples. Analyses of the expression levels of the miRNAs identified a total of 23 and 1 differentially expressed (DE) miRNAs in ovaries and testes, respectively, two months after the heat treatment. Most of the identified miRNAs were involved in human sex-related cancer. After retrieving 3’ UTR regions, ~400 predicted targets of the 24 DE miRNAs were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of them, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the predicted sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.

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Whole transcriptome-based miRNA-mRNA network analysis revealed the mechanism of inflammation-immunosuppressive damage caused by cadmium in common carp spleens

Cited by 81 publications

References 55 publications

Oleanolic acid reshapes the gut microbiota and alters immune‐related gene expression of intestinal epithelial cells

Oleanolic acid reshapes the gut microbiota and alters immune‐related gene expression of intestinal epithelial cells

Whole Transcriptome Profiling of the Effects of Cadmium on the Liver of the Xiangxi Yellow Heifer

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

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