Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity

Tanaka, Nobuyuki; Kanatani, Shigeaki; Tomer, Raju; Sahlgren, Cecilia; Kronqvist, Pauliina; Kaczyńska, Dagmara; Louhivuori, Lauri; Kis, Lóránd; Lindh, Claes; Mitura, Przemysław; Stepulak, Andrzej; Corvigno, Sara; Hartman, Johan; Micke, Patrick; Mezheyeuski, Artur; Strell, Carina; Carlson, Joseph W.; Moro, Carlos Fernández; Dahlstrand, Hanna; Östman, Arne; Matsumoto, Kazuhiro; Wiklund, Peter; Oya, Mototsugu; Miyakawa, Ayako; Deisseroth, Karl; Uhlén, Per

doi:10.1038/s41551-017-0139-0

Cited by 156 publications

(164 citation statements)

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“…The EPR effect that is always claimed to be responsible for the build‐up of nanoentities in malignant tissues is well demonstrated in lab animals but is more heterogeneous in humans, that is, the same tumor type may or may not show uptake of NPs in different patients. This heterogeneous nature of tumors leads to unpredictable uptake and often failure of passive targeting in patients . Therefore, one of the main challenges is understanding the principles governing uptake of nanocarriers in human cancers so that strategies could be developed to exploit or even modify the microenvironment of tumors to ensure better delivery .…”

Section: Clinical Perspectivementioning

confidence: 99%

Enhanced Cancer Therapy by Combining Radiation and Chemical Effects Mediated by Nanocarriers

Denkova

Liu

Men

et al. 2020

Advanced Therapeutics

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Next to surgery, radiotherapy and chemotherapy are the two most commonly applied treatments against cancer. These therapies are often combined, but the therapeutic outcome is still limited. Nanocarriers may be the solution to many of the issues encountered when combining these therapies. In this review, the role of nanocarriers in combined radiotherapy and chemical cancer treatment is discussed, divided into four distinct strategy classes. For each strategy, examples of the literature are given to come eventually to conclusion on the possible translation to the clinic.

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Section: Clinical Perspectivementioning

confidence: 99%

Enhanced Cancer Therapy by Combining Radiation and Chemical Effects Mediated by Nanocarriers

Denkova

Liu

Men

et al. 2020

Advanced Therapeutics

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“…Given the growing interest in LSFM for both fundamental and clinical research, the improved image quality provided by the mDLSM approach should enable improved biological investigations as well as higher-fidelity pathology for accurate prognostication and treatment stratification [10,24,25].…”

Section: Introductionmentioning

confidence: 99%

Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

Glaser

Chen

Wei

et al. 2018

Preprint

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One Sentence Summary: Glaser et al. describe a light-sheet microscopy architecture that enables passive multidirectional illumination with confocal line detection to enable both uniform fluorescence excitation and contrast-enhanced imaging of fluorescently labeled samples.peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission.The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/270207 doi: bioRxiv preprint first posted online Feb. 23, 2018; Abstract Light-sheet fluorescence microscopy (LSFM) has emerged as a powerful method for rapid and optically efficient 3D microscopy. Initial LSFM designs utilized a static sheet of light, termed selective plane illumination microscopy (SPIM), which exhibited shadowing artifacts and deteriorated contrast due to light scattering. These issues have been addressed, in part, by multidirectional selective plane illumination microscopy (mSPIM), in which rotation of the light sheet is used to mitigate shadowing artifacts, and digital scanned light-sheet microscopy (DSLM), in which confocal line detection is used to reject scattered light. Here we present a simple passive multidirectional digital scanned light-sheet microscopy (mDSLM) architecture that combines the benefits of mSPIM and DSLM. By utilizing an elliptical Gaussian beam with increased angular diversity in the imaging plane, mDSLM provides shadow-free contrastenhanced imaging of fluorescently labeled samples.peer-reviewed)

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“…Our experimental data present, first of all, a caveat to the community that, in contrast to the existing literature, the amount of tissue shrinkage by BABB clearing depends in addition to the tissue also on the size of the sample. Secondly, this work shows that it is possible to establish a closed-form empirical description of size dependent shrinkage, which will be instrumental for a quantitatively correct interpretation of images of BABB-cleared biopsies in the clinical practice 10,12,13 . At this point, we do not know the physical mechanism responsible for the observed size dependent clearing.…”

mentioning

confidence: 96%

“…55 % for mouse brains, 3 30 % for hippocampus and cortex layers, and 10.7 % for a mouse torso) 11 . Given that a number of groups are extending the study of tissue analysis in 3D to that of human biopsies, using various means of optical microscopy yet all relying on tissue clearing 10,12,13 , it becomes important to know the extent of tissue shrinkage caused by the use of clearing agents.…”

mentioning

confidence: 99%

Clearing-induced tisssue shrinkage: Novel observation of a thickness size effect

Vulders

Hoogenhuizen

Giessen

et al. 2020

Preprint

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The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D.One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that the shrinkage for a widely-used clearing agent decreases significantly with increasing sample size, and report an analytical description.

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Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity

Cited by 156 publications

References 50 publications

Enhanced Cancer Therapy by Combining Radiation and Chemical Effects Mediated by Nanocarriers

Enhanced Cancer Therapy by Combining Radiation and Chemical Effects Mediated by Nanocarriers

Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

Clearing-induced tisssue shrinkage: Novel observation of a thickness size effect

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