Whole slide imaging for human epidermal growth factor receptor 2 immunohistochemistry interpretation: Accuracy, Precision, and reproducibility studies for digital manual and paired glass slide manual interpretation

Wilbur, David C.; Brachtel, Elena F.; Gilbertson, John R.; Jones, Nicholas; Vallone, John; Krishnamurthy, Savitra

doi:10.4103/2153-3539.157788

Cited by 12 publications

(12 citation statements)

References 28 publications

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“…Additionally, it is necessary to note that applying a digital immunohistochemistry approach with the purpose to objectively detect and estimate tissue markers studied, as stated in the material and methods, we obtained results comparable with the manually obtained data. Similarly, no statistical difference between the variance of performance when comparing glass and digital interpretations was found by other researchers [65,66]. Still, it seems that, when applying a digital approach, one should be careful to focus on specific pathology tasks to reduce sources of variability diluting findings [67], and prevent escalation of data size and complexity developing in well-designed databases [68].…”

Section: Discussionmentioning

confidence: 93%

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

Upeniece

Groma

Skuja

et al. 2016

pjp

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The study of cytoskeleton arrangement and its contribution to survival of cell-tocell contacts appears to be essential for understanding of numerous cellular and tissue processes. Applying CK15, S100 labeling and TUNEL reaction to cutaneous lichen planus subtypes, we found CK15 expression in the outer and inner root sheath of hair follicles, the basal epidermal layer, and eccrine glands. Its follicular expression was decreased in nearby inflammatory infiltrates. The CK15 immunopositivity was mostly described as weak (92.3%) for lichen planus but equally subdivided into weak, moderate and strong in lichen planopilaris (χ 2 = 32.514; df = 4; p < 0.001). The greatly varying apoptotic index was the highest in the lichen planopilaris involving the scalp: 81.2 ±10.7; 87.8 ±10.7 and 88.0 ±10.5 for the basal, spinous and upper epidermal layers, respectively. S100 positive epidermal and follicular cells did not differ in the lesions demonstrated in the study groups; still immunoreactivity was more pronounced in the scalp region of lichen planopilaris. Damage of cell-to-cell contacts was confirmed by electron microscopy. Apart from immunocyte-mediated keratinocyte death, cytoskeleton-based injury and loss of cellto-cell and matrix contacts may be of great importance, leading to eradication of degrading cells and thus contributing to the pathogenesis of lichen planus.

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Section: Discussionmentioning

confidence: 93%

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

Upeniece

Groma

Skuja

et al. 2016

pjp

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“…The use of the HercepTest slides for scanner quality evaluation was driven by another associated experiment designed to analyze diagnostic accuracy of pathologists using a WSI system. [12] Since the diagnosis of HercepTest slides is reported in terms of quantitative scores, instead of qualitative descriptions, it allows application of objective and statistical methods for analyzing diagnostic accuracy and reproducibility. However, the proposed method is generic and also applicable to other types of tissues and stain types.…”

Section: Methodsmentioning

confidence: 99%

A quantitative approach to evaluate image quality of whole slide imaging scanners

Shrestha

Kneepkens

Vrijnsen

et al. 2016

Journal of Pathology Informatics

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Context:The quality of images produced by whole slide imaging (WSI) scanners has a direct influence on the readers’ performance and reliability of the clinical diagnosis. Therefore, WSI scanners should produce not only high quality but also consistent quality images.Aim:We aim to evaluate reproducibility of WSI scanners based on the quality of images produced over time and among multiple scanners. The evaluation is independent of content or context of test specimen.Methods:The ultimate judge of image quality is a pathologist, however, subjective evaluations are heavily influenced by the complexity of a case and subtle variations introduced by a scanner can be easily overlooked. Therefore, we employed a quantitative image quality assessment method based on clinically relevant parameters, such as sharpness and brightness, acquired in a survey of pathologists. The acceptable level of quality per parameter was determined in a subjective study. The evaluation of scanner reproducibility was conducted with Philips Ultra-Fast Scanners. A set of 36 HercepTest™ slides were used in three sub-studies addressing variations due to systems and time, producing 8640 test images for evaluation.Results:The results showed that the majority of images in all the sub-studies are within the acceptable quality level; however, some scanners produce higher quality images more often than others. The results are independent of case types, and they match our perception of quality.Conclusion:The quantitative image quality assessment method was successfully applied in the HercepTest™ slides to evaluate WSI scanner reproducibility. The proposed method is generic and applicable to any other types of slide stains and scanners.

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“…Furthermore, under‐staining and over‐staining can lead to difficulties in scanning IHC‐stained slides and/or interpretation of results. Inconsistent staining and heterogenous staining of slides is a challenge for automated image analysis and automated IHC‐scoring . This may be addressed by the use of control slides for calibration purposes in future studies.…”

Section: Database Archtechture and Viewing Systemmentioning

confidence: 99%

mentioning

confidence: 99%

“…Digital pathology has started to assume an important role in pathology diagnosis, especially the use of whole slide imaging (WSI), which allows for consistent quantitative analysis of histological slides. [1][2][3] Whole slide images are generated by robotic, microscope-like devices that scan and digitize glass slides. There are currently around twenty commercially available devices that are capable of scanning glass slides and combining individual scanned fields into a single composite image file.…”

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confidence: 99%

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Development of a database system and image viewer to assist in the correlation of histopathologic features and digital image analysis with clinical and molecular genetic information

Yagi

Riedlinger

et al. 2016

Pathology International

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Pathologists are required to integrate data from multiple sources when making a diagnosis. Furthermore, whole slide imaging (WSI) and next generation sequencing will escalate data size and complexity. Development of well-designed databases that can allow efficient navigation between multiple data types is necessary for both clinical and research purposes. We developed and evaluated an interactive, webbased database that integrates clinical, histologic, immunohistochemical and genetic information to aid in pathologic diagnosis and interpretation with nine lung adenocarcinoma cases. To minimize sectioning artifacts, representative blocks were serially sectioned using automated tissue sectioning (Kurabo Industries, Osaka Japan) and selected slides were stained by multiple techniques, (hematoxylin and eosin [H&E], immunohistochemistry [IHC] or fluorescence in situ hybridization [FISH]). Slides were digitized by WSI scanners. An interactive relational database was designed based on a list of proposed fields covering a variety of clinical, pathologic and molecular parameters. By focusing on the three main tasks of 1.) efficient management of textual information, 2.) effective viewing of all varieties of stained whole slide images (WSI), and 3.) assistance in evaluating WSI with computer-aided diagnosis, this database prototype shows great promise for multi-modality research and diagnosis.Key words: database management system, genetic information, lung adenocarcinoma, image analysis, histopathology Digital pathology has started to assume an important role in pathology diagnosis, especially the use of whole slide imaging (WSI), which allows for consistent quantitative analysis of histological slides.1-3 Whole slide images are generated by robotic, microscope-like devices that scan and digitize glass slides. There are currently around twenty commercially available devices that are capable of scanning glass slides and combining individual scanned fields into a single composite image file. Due to the interest of many researchers from around the globe, the technology has advanced since it was first introduced more than about twenty years ago. We have been involved in the development of advanced WSI scanners since then and related technologies instrumental for imaging projects. Whole slide images produced today are of excellent quality and may be shared over the internet or through data storage devices. Image viewing software permits navigation, editing, annotation, and analysis of these images. However, image analysis results are often affected by the quality of whole slide images, and the quality of whole slide images are affected by the quality of tissue slides. For example, in our previous study 4 we found that consistent thickness of tissue slides and absence of tissue artifacts will result in good image quality and consistent analysis results.As WSI technology improves, it has the potential to contribute novel and complex diagnostic discoveries. For example, in pathology, the relationship between an observed histomorph...

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Whole slide imaging for human epidermal growth factor receptor 2 immunohistochemistry interpretation: Accuracy, Precision, and reproducibility studies for digital manual and paired glass slide manual interpretation

Cited by 12 publications

References 28 publications

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

A quantitative approach to evaluate image quality of whole slide imaging scanners

Development of a database system and image viewer to assist in the correlation of histopathologic features and digital image analysis with clinical and molecular genetic information

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