Whole-Section Tumor Micro-Architecture Analysis by a Two-Dimensional Phasor-Based Approach Applied to Polarization-Dependent Second Harmonic Imaging

Scodellaro, Riccardo; Bouzin, Margaux; Mingozzi, Francesca; D’Alfonso, Laura; Granucci, Francesca; Collini, Maddalena; Chirico, Giuseppe; Sironi, Laura

doi:10.3389/fonc.2019.00527

Cited by 16 publications

(8 citation statements)

References 79 publications

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“…The collagen, accounting for 70-80% of dry skin weight, is the main component of the extracellular matrix [29,30] and can be employed as an indicator in the process of dermal repair. Collagen imaging has been widely used in the transdermal movement of drugs in vivo [31], scar prognosis [32,33], and cancerous tissues [34,35]. This study showed that b-FGF accelerated collagen production by TMP imaging in vivo, which was consistent with the Masson staining results in vitro.…”

Section: Discussionsupporting

confidence: 80%

Assessing Skin Healing and Angiogenesis of Deep Burns in Vivo Using Two-Photon Microscopy in Mice

Cao

Tang

et al. 2022

Front. Phys.

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Prevalent methods for monitoring burn injuries and testing drug efficacies rely on fixed tissue sections. However, this may leave out cellular details in the living state. In vivo assessments of burn healing has been long sought after and are of scientific and clinical interest. Nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), collagen, and melanin are endogenous fluorescent molecules and their signals can be captured by two-photon microscopy (TPM), therefore providing information on epidermal histological features and collagen growth in real-time. In addition, TMP imaging on exogenous fluorescent substances provides a basis for detecting blood vessels. In this work, two-photon microscopy was used to capture the exogenous fluorescent substances and endogenous fluorescent molecules at different times to assess and track burn healing in vivo. Combining TPM imaging and morphological characteristics, proliferation and differentiation of the keratinocytes in different layers of skin, collagen contents, and angiogenesis were identified and quantified. The TPM monitoring method provides an effective tool to systemically evaluate skin healing of deep burns in vivo.

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Section: Discussionsupporting

confidence: 80%

Assessing Skin Healing and Angiogenesis of Deep Burns in Vivo Using Two-Photon Microscopy in Mice

Cao

Tang

et al. 2022

Front. Phys.

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“…The spatial arrangement of collagen in stroma has also been observed in breast cancer and liver metastasis tissue samples using second harmonic generation spectroscopy (Scodellaro et al, 2019;Wang et al, 2019). Collagen patterns are associated with degree of tumor invasiveness (Pankova et al, 2016;Provenzano et al, 2008).…”

Section: Collagenmentioning

confidence: 98%

Molecular imaging of extracellular matrix proteins with targeted probes using magnetic resonance imaging

Salarian

Ibhagui

Yang

2020

WIREs Nanomed Nanobiotechnol

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The extracellular matrix (ECM) consists of proteins and carbohydrates that supports different biological structures and processes such as tissue development, elasticity, and preservation of organ structure. Diseases involving inflammation, fibrosis, tumor invasion, and injury are all attributed to the transition of the ECM from homeostasis to remodeling, which can significantly change the biochemical and biomechanical features of ECM components. While contrast agents have played an indispensable role in facilitating clinical diagnosis of diseases using magnetic resonance imaging (MRI), there is a strong need to develop novel biomarker‐targeted imaging probes for in vivo visualization of biological processes and pathological alterations at a cellular and molecular level, for both early diagnosis and monitoring drug treatment. Herein, we will first review the pathological accumulation and characterization of ECM proteins recognized as important molecular features of diseases. Developments in MRI probes targeting ECM proteins such as collagen, fibronectin, and elastin via conjugation of existing contrast agents to targeting moieties and their applications to various diseases, are also reviewed. We have also reviewed our progress in the development of collagen‐targeted protein MRI contrast agent with significant improvement in relaxivity and metal binding specificity, and their applications in early detection of fibrosis and metastatic cancer. This article is categorized under: Diagnostic Tools > in vivo Nanodiagnostics and Imaging Biology‐Inspired Nanomaterials > Peptide‐Based Structures Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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“…Interestingly, analysis of the PSHG data has also been achieved through Fourier projection of the PSHG image stacks onto two phasor plots referred to as microscopic multiparametric analysis by phasor projection of PSHG (µMAPPS). This technique has been used to compare the ρ values of SHG data taken from biopsies of the left flank of mice days after implantation of melanoma [40], colon carcinoma and breast cancer cells [41].…”

Section: Applications Of Shg Microscopy For Determining the Ultrastructure Of Diseased Collagenous Tissues Polarization-in Shg Microscopymentioning

confidence: 99%

Polarization-Sensitive Second Harmonic Generation Microscopy for Investigations of Diseased Collagenous Tissues

et al. 2021

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The advancement of non-invasive quantitative optical diagnosis techniques such as polarization-sensitive second harmonic generation microscopy (PSHG) for diseases such as cancer presents opportunities for improving disease understanding and survival rates. Here, novel and developing techniques in PSHG microscopy applied for the differentiation of cancerous or diseased tissues are presented, including circular dichroism, modulation of laser linear polarization, detection of outgoing linear laser polarization, and double-Stokes Mueller. Typically, initial cancer diagnosis is performed by visual inspection of stained biopsy or surgical resection tissue sections under bright-field microscopy, however, early diagnosis is challenging due to variability in morphological interpretation of the tissues, and because cancer initiation regions can be small and easy to miss. Therefore, pathologists could benefit in identifying cancer on biopsy or surgical resection sections by using unbiased quantitative automated technologies with high spatial resolution and improved disease specificity that can check the entire slide pixel-by-pixel. Second harmonic generation microscopy offers the opportunity to measure ultrastructural alterations in collagenous scaffolds of organ tissues virtually background free on submicron-sized tissue regions. The approach is particularly interesting for cancer diagnosis applications, because during cancer initiation and progression, the collagen in the affected tissue extracellular matrix is often deregulated and becomes disorganized. This mini-review contains a thorough summary of PSHG techniques that have interrogated diseased tissues, and discusses their technical variations and successes in disease discrimination.

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Whole-Section Tumor Micro-Architecture Analysis by a Two-Dimensional Phasor-Based Approach Applied to Polarization-Dependent Second Harmonic Imaging

Cited by 16 publications

References 79 publications

Assessing Skin Healing and Angiogenesis of Deep Burns in Vivo Using Two-Photon Microscopy in Mice

Assessing Skin Healing and Angiogenesis of Deep Burns in Vivo Using Two-Photon Microscopy in Mice

Molecular imaging of extracellular matrix proteins with targeted probes using magnetic resonance imaging

Polarization-Sensitive Second Harmonic Generation Microscopy for Investigations of Diseased Collagenous Tissues

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