Whole-organ bioengineering: current tales of modern alchemy

Moran, Emma; Dhal, Abritee; Vyas, Dipen; Lanas, Fernando; Söker, Shay; Baptista, Pedro M.

doi:10.1016/j.trsl.2014.01.004

Cited by 24 publications

(14 citation statements)

References 60 publications

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“…[239] However, the current knowledge and technology is still limited, making the transplants viable for a few hours or at best a few days, due to blood clotting and poor revascularisation. [240] Cell seeding via continuous perfusion can deliver large cell quantities into the whole scaffold. However, long perfusion times may potentially damage the scaffold due to continuous exposure to elevated shear during the seeding.…”

Section: Liver Recellularizationmentioning

confidence: 99%

Bioengineering Organs for Blood Detoxification

Legallais

Kim

Mihaila

et al. 2018

Adv Healthcare Materials

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For patients with severe kidney or liver failure the best solution is currently organ transplantation. However, not all patients are eligible for transplantation and due to limited organ availability, most patients are currently treated with therapies using artificial kidney and artificial liver devices. These therapies, despite their relative success in preserving the patients' life, have important limitations since they can only replace part of the natural kidney or liver functions. As blood detoxification (and other functions) in these highly perfused organs is achieved by specialized cells, it seems relevant to review the approaches leading to bioengineered organs fulfilling most of the native organ functions. There, the culture of cells of specific phenotypes on adapted scaffolds that can be perfused takes place. In this review paper, first the functions of kidney and liver organs are briefly described. Then artificial kidney/liver devices, bioartificial kidney devices, and bioartificial liver devices are focused on, as well as biohybrid constructs obtained by decellularization and recellularization of animal organs. For all organs, a thorough overview of the literature is given and the perspectives for their application in the clinic are discussed.

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Section: Liver Recellularizationmentioning

confidence: 99%

Bioengineering Organs for Blood Detoxification

Legallais

Kim

Mihaila

et al. 2018

Adv Healthcare Materials

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“…A promising approach for creating artificial organs is decellularization of allogeneic or xenogeneic organs to clear away all potential immunogens followed by recellularization with patient-specific cells to regenerate whole healthy organs. 5–7 …”

Section: Introductionmentioning

confidence: 99%

Re-epithelialization of whole porcine kidneys with renal epithelial cells

et al. 2017

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Decellularized porcine kidneys were recellularized with renal epithelial cells by three methods: perfusion through the vasculature under high pressure, perfusion through the ureter under high pressure, or perfusion through the ureter under moderate vacuum. Histology, scanning electron microscopy, confocal microscopy, and magnetic resonance imaging were used to assess vasculature preservation and the distribution of cells throughout the kidneys. Cells were detected in the magnetic resonance imaging by labeling them with iron oxide. Perfusion of cells through the ureter under moderate vacuum (40 mmHg) produced the most uniform distribution of cells throughout the kidneys.

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“…Several studies have already demonstrated the possibility of successfully obtaining acellular scaffolds from many organs, such as heart, kidney, pancreas, lung, liver, esophagus and intestine. Importantly, some of these decellularization protocols have been adopted to decellularize simple hollow organs such as the trachea, which have then been successfully transplanted in patient after autologous cell seeding, i.e., trachea [4,5,6]. Importantly, the trachea transplant has been achieved without immunosuppression, a great advantage over conventional transplantation because it avoids potential risks for patients, including frequent infections and cancer.…”

Section: Introductionmentioning

confidence: 99%

Decellularized Tissue for Muscle Regeneration

Urciuolo

Coppi²

2018

IJMS

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Several acquired or congenital pathological conditions can affect skeletal muscle leading to volumetric muscle loss (VML), i.e., an irreversible loss of muscle mass and function. Decellularized tissues are natural scaffolds derived from tissues or organs, in which the cellular and nuclear contents are eliminated, but the tridimensional (3D) structure and composition of the extracellular matrix (ECM) are preserved. Such scaffolds retain biological activity, are biocompatible and do not show immune rejection upon allogeneic or xenogeneic transplantation. An increase number of reports suggest that decellularized tissues/organs are promising candidates for clinical application in patients affected by VML. Here we explore the different strategies used to generate decellularized matrix and their therapeutic outcome when applied to treat VML conditions, both in patients and in animal models. The wide variety of VML models, source of tissue and methods of decellularization have led to discrepant results. Our review study evaluates the biological and clinical significance of reported studies, with the final aim to clarify the main aspects that should be taken into consideration for the future application of decellularized tissues in the treatment of VML conditions.

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Whole-organ bioengineering: current tales of modern alchemy

Cited by 24 publications

References 60 publications

Bioengineering Organs for Blood Detoxification

Bioengineering Organs for Blood Detoxification

Re-epithelialization of whole porcine kidneys with renal epithelial cells

Decellularized Tissue for Muscle Regeneration

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