Whole‐mount pathology of breast lumpectomy specimens improves detection of tumour margins and focality

Clarke, Geoff; Holloway, Claire; Zubovits, Judit; Nofech‐Mozes, Sharon; Liu, Kela; Murray, Mayan; Wang, Dan; Yaffe, Martin J.

doi:10.1111/his.12912

Cited by 8 publications

(3 citation statements)

References 51 publications

(87 reference statements)

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“…An alternative approach to ITH model building is to completely resect the affected organ, either by radical surgery [98][99][100][101] or by waiting until the patient is deceased and performing autopsy and tissue extraction using pre-defined target regions. 102,103 Compared with stereotactic biopsy, this method has the advantages of near unlimited tissue sampling granularity and the ability to evaluate healthy appearing regions suspected of harboring microscopic disease and/or epigenetic abnormalities that may not be safe to study while the patient is alive.…”

Section: Whole-mount/brain Autopsymentioning

confidence: 99%

Targeting intra‐tumoral heterogeneity of human brain tumors with in vivo imaging: A roadmap for imaging genomics from multiparametric MR signals

et al. 2022

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Resistance of high grade tumors to treatment involves cancer stem cell features, deregulated cell division, acceleration of genomic errors, and emergence of cellular variants that rely upon diverse signaling pathways. This heterogeneous tumor landscape limits the utility of the focal sampling provided by invasive biopsy when designing strategies for targeted therapies. In this roadmap review paper, we propose and develop methods for enabling mapping of cellular and molecular features in vivo to inform and optimize cancer treatment strategies in the brain. This approach leverages (1) the spatial and temporal advantages of in vivo imaging compared with surgical biopsy, (2) the rapid expansion of meaningful anatomical and functional magnetic resonance signals, (3) widespread access to cellular and molecular information enabled by next-generation sequencing, and (4) the enhanced accuracy and computational efficiency of deep learning techniques. As multiple cellular variants may be present within volumes below the resolution of imaging, we describe a mapping process to decode micro-and even nano-scale properties from the macro-scale data by simultaneously utilizing complimentary multiparametric image signals acquired in routine clinical practice. We outline design protocols for future research efforts that marry revolutionary bioinformation technologies, growing access to increased computational capability, and powerful statistical classification techniques to guide rational treatment selection.

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Section: Whole-mount/brain Autopsymentioning

confidence: 99%

Targeting intra‐tumoral heterogeneity of human brain tumors with in vivo imaging: A roadmap for imaging genomics from multiparametric MR signals

et al. 2022

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“…First, the sensitivity and specificity of positive margin detection were compared for the wm and conventional techniques, and then the consequences of repeated surgery for positive margins was modelled. Sensitivity and specificity values for wm were defined based on a "positive margin" being the presence of tumour cells 0.1 mm or less from the cut edge 16 , with sensitivity analyses (discussed later in this section).…”

Section: Model Structurementioning

confidence: 99%

Cost-Effectiveness Analysis of Whole-Mount Pathology Processing for Patients with Early Breast Cancer Undergoing Breast Conservation

et al. 2016

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“…Consequently, this not only enables validation of any residual tumor but would also help identify what may have been previously unsuspected significant findings [5]. Large format histology is suitable for resected tissues and whole organs such as breast, prostate and gastrointestinal tract in both clinical and research settings [3,[6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Application of large format tissue processing in the histology laboratory

Bryant

Haine²,

Johnston³

et al. 2019

Journal of Histotechnology

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In clinical, research and veterinary laboratories of North America, large format histology has more recently been improved with newer equipment and better methodology. Large tissue specimens are frequently sliced in the grossing room and processed in multiple smaller, standard size tissue cassettes. Justifiably, submitting more blocks inherently lends itself to a greater confidence in the accuracy of the diagnosis, yet guidelines for tissue sampling often suggest taking fewer samples. For example, large tumor specimen protocols recommend taking one standard sized tissue block for each cm diameter of tumor. However, cancers are the culmination of many complex changes in cell metabolism and often appear dissimilar at different tissue locations. As these changes have an uncertain behaviour, many other tissue samples are often taken from areas that appear to have either a variable texture or color. Consequently, at microscopy, the complete tissue sample may need to be reassembled like a jigsaw puzzle as the stained sections are frequently presented over many slides. This problem has easily been overcome by using large format cassettes since the entire cross-section of the tissue sample can often be viewed on a single slide. Because these cassettes can effectively hold up to ten times the volume of conventional standard size cassettes, they are a more efficient way of assessing large areas of tissue samples. This system is easily adapted for all tissue types and has become the established method for assessing large tissue samples in many laboratory settings.

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Whole‐mount pathology of breast lumpectomy specimens improves detection of tumour margins and focality

Cited by 8 publications

References 51 publications

Targeting intra‐tumoral heterogeneity of human brain tumors with in vivo imaging: A roadmap for imaging genomics from multiparametric MR signals

Targeting intra‐tumoral heterogeneity of human brain tumors with in vivo imaging: A roadmap for imaging genomics from multiparametric MR signals

Cost-Effectiveness Analysis of Whole-Mount Pathology Processing for Patients with Early Breast Cancer Undergoing Breast Conservation

Application of large format tissue processing in the histology laboratory

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