Whole genome sequencing of the highly virulent subtype B0/W148 of the Beijing genotype M.tuberculosis in Kharkiv, East Ukraine

Completely Free from PAM Limitations: Asymmetric RPA with CRISPR/Cas12a for Nucleic Acid Assays

“…8 Therefore, it is particularly important to distinguish between drug-resistant and wild-type strains for the right medicine. TB drug susceptibility testing includes solid or liquid culture, 9 sequencing, 10 and realtime quantitative PCR (qPCR). 11 However, these methods are time-consuming and require professional personnel.…”

Rapid and Ultrasensitive Approach for the Simultaneous Detection of Multilocus Mutations to Distinguish Rifampicin-Resistant Mycobacterium tuberculosis

“…Also, the detection of single-nucleotide variation (SNV) is very difficult due to the lack of high specificity. TB drug susceptibility testing (DST) commonly relies on solid or liquid culture, 4,15 sequencing, 16…”

“…Also, the detection of single-nucleotide variation (SNV) is very difficult due to the lack of high specificity. TB drug susceptibility testing (DST) commonly relies on solid or liquid culture, , sequencing, and real-time quantitative PCR (qPCR) . The solid or liquid culture takes weeks to get results, which is time-consuming.…”