Whole-genome sequencing of Pseudomonas sp. TAE6080, a strain capable of inhibiting Staphylococcus epidermidis biofilm

Riccardi, Christopher; D’Angelo, Caterina; Calvanese, Marzia; Ricciardelli, Annarita; Sellitto, Assunta; Giurato, Giorgio; Tutino, Maria; Weisz, Alessandro; Parrilli, Ermenegilda; Fondi, Marco

doi:10.1016/j.margen.2021.100887

Cited by 4 publications

(12 citation statements)

References 22 publications

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“…Pseudomonas sp. TAE6080 cell-free supernatant was evaluated since it has been previously tested on S. epidermidis RP62A biofilm formation, demonstrating that it significantly reduced aggregation in this process; moreover, genome sequencing of this strain revealed the presence of putative biosynthetic gene clusters that might be involved in biofilm destabilization [20]. The last strain selected was Pseudoalteromonas haloplanktis TAD2020; the cell-free supernatant of this marine bacteria can interfere with S. epidermidis biofilm formation (unpublished results).…”

Section: Selection Of Antarctic Marine Bacteriamentioning

confidence: 99%

“…TAE2020 the most simple medium resulted to be GLUT [26], in the case of Pseudomonas sp. TAE6080 the best solution was GLUC [20].…”

Section: Effect Of Antarctic Cell-free Supernatants On Biofilmmentioning

confidence: 99%

“…Biofilm formation allows cells to grow even in oligotrophic environments [16,17], and the production of anti-biofilm molecules can reduce the biofilm formation of competitors and their ability to survive. Therefore, it is not surprising that some recent papers report that marine Antarctic bacteria produce and secrete anti-biofilm molecules [13,[18][19][20][21][22][23]. Furthermore, cell-free supernatants and organic extracts obtained from different bacterial cultures of Polar marine bacteria showed interesting anti-biofilm activities on P. aeruginosa and S. aureus [13,19,22].…”

Section: Introductionmentioning

confidence: 99%

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Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

Artini,

Papa,

Vrenna

et al. 2023

Antibiotics

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The ESKAPE pathogens, including bacteria such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species, pose a global health threat due to their ability to resist antimicrobial drugs and evade the immune system. These pathogens are responsible for hospital-acquired infections, especially in intensive care units, and contribute to the growing problem of multi-drug resistance. In this study, researchers focused on exploring the potential of Antarctic marine bacteria as a source of anti-biofilm molecules to combat ESKAPE pathogens. Four Antarctic bacterial strains were selected, and their cell-free supernatants were tested against 60 clinical ESKAPE isolates. The results showed that the supernatants did not exhibit antimicrobial activity but effectively prevented biofilm formation and dispersed mature biofilms. This research highlights the promising potential of Antarctic bacteria in producing compounds that can counteract biofilms formed by clinically significant bacterial species. These findings contribute to the development of new strategies for preventing and controlling infections caused by ESKAPE pathogens.

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Section: Selection Of Antarctic Marine Bacteriamentioning

confidence: 99%

“…TAE2020 the most simple medium resulted to be GLUT [26], in the case of Pseudomonas sp. TAE6080 the best solution was GLUC [20].…”

Section: Effect Of Antarctic Cell-free Supernatants On Biofilmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

Artini,

Papa,

Vrenna

et al. 2023

Antibiotics

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show abstract

“…Biofilm formation allows cells to grow even in highly oligotrophic environments [10,11], and the production of anti-biofilm molecules can reduce the biofilm formation of competitors and their ability to survive. Therefore, is not a surprise that some recent papers report that marine Antarctic bacteria produce and secrete anti-biofilm molecules [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…difficult to uptake. Biofilm fo environments [10,11], and the p formation of competitors and t recent papers report that ma molecules [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020

et al. 2022

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The development of new approaches to prevent microbial surface adhesion and biofilm formation is an emerging need following the growing understanding of the impact of biofilm-related infections on human health. Staphylococcus epidermidis, with its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in infections of medical devices. In the research of new anti-biofilm agents against S. epidermidis biofilm, Antarctic marine bacteria represent an untapped reservoir of biodiversity. In the present study, the attention was focused on Psychrobacter sp. TAE2020, an Antarctic marine bacterium that produces molecules able to impair the initial attachment of S. epidermidis strains to the polystyrene surface. The setup of suitable purification protocols allowed the identification by NMR spectroscopy and LC-MS/MS analysis of a protein–polysaccharide complex named CATASAN. This complex proved to be a very effective anti-biofilm agent. Indeed, it not only interferes with cell surface attachment, but also prevents biofilm formation and affects the mature biofilm matrix structure of S. epidermidis. Moreover, CATASAN is endowed with a good emulsification activity in a wide range of pH and temperature. Therefore, its use can be easily extended to different biotechnological applications.

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Cold-Azurin a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium <em>Pseudomonas</em> sp. TAE6080

D’Angelo,

Trecca,

Carpentieri

et al. 2024

Preprint

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Bacteria can colonize and develop biofilm on a large variety of biotic and abiotic materials, it is accountable for nosocomial infections and chronic illness and consequently, it is a serious economic and public health problem. Staphylococcus epidermidis, thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in bio-film-associated infections of medical devices. Due to the biofilm presence, S. epidermidis infections are extremely difficult to treat and the research of new molecules able to interfere with its biofilm formation has a remarkable interest. In the present work, the attention was focused on Pseudomonas sp. TAE6080, an Antarctic marine bacterium able to produce and secrete an effective antibiofilm compound. The molecule responsible for this activity was purified by an activity-guided approach and it resulted to be a periplasmic protein, named Cold-Azurin. The Cold-Azurin was recombinantly produced in E. coli and purified. The recombinant protein was able to impair S. epidermidis attachment to the polystyrene surface and effectively prevent biofilm formation.

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Whole-genome sequencing of Pseudomonas sp. TAE6080, a strain capable of inhibiting Staphylococcus epidermidis biofilm

Cited by 4 publications

References 22 publications

Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020

Cold-Azurin a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium <em>Pseudomonas</em> sp. TAE6080

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