Whole genome sequencing of OXA-232-producing wzi93-KL112-O1 carbapenem-resistant Klebsiella pneumoniae in human bloodstream infection co-harboring chromosomal ISEcp1-based blaCTX-M-15 and one rmpA2-associated virulence plasmid

Tian, Chongmei; Xing, Mengyu; Zhao, Yaping; Fan, Xueyu; Bai, Yang; Fu, Liping; Wang, Siwei

doi:10.3389/fcimb.2022.984479

Cited by 4 publications

(2 citation statements)

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“…K. pneumoniae , as a vital human pathogen, represents increasing multidrug-resistance, particularly to third-generation cephalosporins and carbapenems [ 11 ]. Taking into account the mortality and morbidity of patients, medical burden, global drug resistance trends, and other relevant standards, CRKP was undoubtedly considered a key priority pathogen in the priority antibiotic-resistant bacteria list by the World Health Organization (WHO) in 2017 [ 3 , 4 ].…”

Section: Discussionmentioning

confidence: 99%

“…In existing research reports, most CR-hvKP strains harboring the bla KPC-2 plasmid were identified in China [ 5 , 6 , 7 ]. However, CR-hvKP strains bearing bla OXA-48 have never been documented in Hainan, China [ 11 , 12 ]. In this research, we aimed to determine the clinical, drug-resistant, microbiological, and genomic characteristics of OXA-48-bearing CR-hvKP strains in the First Affiliated Hospital of Hainan Medical University, China.…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED: Drug Resistance and Molecular Characteristics of Carbapenem-Resistant OXA-48-Producing Klebsiella pneumoniae Strains in Hainan, China

Ye,

Liu,

Liu

et al. 2023

Microorganisms

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Background: The emergence and global spread of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) are of great concern to health services worldwide. These β-lactamases hydrolyze almost all β-lactams, are plasmid-encoded, and are easily transferable among bacterial species. They are mostly of the KPC types in CR-hvKp. OXA-48-producing hvKP strains have been rarely reported in the literature. Methods: OXA-48-producing hvKP strains were collected from clinical specimens at the First Affiliated Hospital of Hainan Medical University from January 2022 to March 2023. Hypervirulent strains were tested for virulence in a mouse lethality study and underwent whole genome sequencing to identify genomic features. Results: A total of 42 unique OXA-48-bearing K. pneumoniae strains were identified, including three CR-hvKP strains (KP2683-1, NCRE61, and KP2185), which were isolated from bacteremia, pulmonary abscess, and liver abscess separately. The three CR-hvKP strains belonged to two different clones of ST11 KL64 (KP2185 and NCRE61) and ST23 K1 (KP2683-1). The KP2683-1 strain had the highest virulence. Whole genome sequencing analysis indicated that NCRE61 and KP2185 acquired IncFIB-type plasmids with a set of virulence genes (iroBCDN, iucABCD, iutA, rmpA, and rmpA2), while KP2683-1 acquired an IncL-type blaOXA-48-harboring plasmid. Consecutive cultures showed that the blaOXA-48-harboring plasmids were highly stable in the three hvKP strains and could be transmitted to Escherichia coli J53 by conjugation. The drug susceptibility testing results show that Ceftazidime/avibactam is sensitive for OXA-48-producing hvKP. Conclusions: Our study highlighted the two evolutionary pathways of OXA-48-producing hvKP strains and confirmed their virulence through in vivo testing. Ceftazidime/avibactam may be a viable option for treating OXA-48-producing hvKP strains.

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Section: Discussionmentioning

confidence: 99%