Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar

Aung, Htin; Tun, Thanda; Moradigaravand, Danesh; Köser, Claudio U.; Nyunt, Wint Wint; Aung, Si Thu; Lwin, Thandar; Thinn, Kyi Kyi; Crump, John A.; Parkhill, Julian; Peacock, Sharon J.; Cook, Gregory M.; Hill, Philip C.

doi:10.1016/j.jgar.2016.04.008

Cited by 28 publications

(24 citation statements)

References 15 publications

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“…We have recently sequenced 18, seven and five Mtb isolates from the New Zealand Rangipo, Otara and Southern Cross clusters, respectively, on the Illumina MiSeq platform (Mac Aogáin et al, 2016;Gautam et al, 2017;Mulholland et al, 2017). An additional four Rangipo genomes were sequenced for this study using paired-end 250-bp reads on the Illumina MiSeq platform using the Nextera TM XT DNA Library Preparation Kit (Illumina Inc., CA, United States) as previously described (Aung et al, 2016). These four strains have been previously sequenced on the SOLiD platform (Colangeli et al, 2014) and were re-sequenced here on Illumina for inclusion in this study.…”

Section: New Zealand L44 Genomesmentioning

confidence: 99%

Dispersal of Mycobacterium tuberculosis Driven by Historical European Trade in the South Pacific

et al. 2019

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Mycobacterium tuberculosis (Mtb) is a globally distributed bacterial pathogen whose population structure has largely been shaped by the activities of its obligate human host. Oceania was the last major global region to be reached by Europeans and is the last region for which the dispersal and evolution of Mtb remains largely unexplored. Here, we investigated the evolutionary history of the Euro-American L4.4 sublineage and its dispersal to the South Pacific. Using a phylodynamics approach and a dataset of 236 global Mtb L4.4 genomes we have traced the origins and dispersal of L4.4 strains to New Zealand. These strains are predominantly found in indigenous Māori and Pacific people and we identify a clade of European, likely French, origin that is prevalent in indigenous populations in both New Zealand and Canada. Molecular dating suggests the expansion of European trade networks in the early 19th century drove the dispersal of this clade to the South Pacific. We also identify historical and social factors within the region that have contributed to the local spread and expansion of these strains, including recent Pacific migrations to New Zealand and the rapid urbanization of Māori in the 20th century. Our results offer new insight into the expansion and dispersal of Mtb in the South Pacific and provide a striking example of the role of historical European migrations in the global dispersal of Mtb.

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Section: New Zealand L44 Genomesmentioning

confidence: 99%

Dispersal of Mycobacterium tuberculosis Driven by Historical European Trade in the South Pacific

et al. 2019

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“…This would also reduce the number of effective drugs to only two (ethionamide and cycloserine) in the standard MDR regimen. This underscores the need to introduce second-line DST in routine diagnosis in Myanmar as described previously (6) to enable construction of effective regimens for treatment of drug-resistant TB patients. Doing so will also allow estimation of the prevalence of extensively drug-resistant TB in the country despite the first reported case in 2007 (7).…”

Section: Genome Announcementmentioning

confidence: 66%

Draft Genome Sequences of Two Drug-Resistant Mycobacterium tuberculosis Isolates from Myanmar

et al. 2016

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“…Estos resultados difieren de trabajos previos publicados en los cuales solo se reporta la presencia de perfiles de mutación en la posición S315T en aislados resistentes 7,19,20,22,31 , el 25,5% de aislados fenotipicamente resistentes que no presentaron la mutación estudiada, pudo deberse a la presencia de polimorfismos en regiones diferente a la posición S315T [15][16][17][18][19][20] en el gen katG u otros genes que aporten resistencia a Isoniacida 9-14 indiciendo a la sobreexpresión que superen el poder inhibitorio de INH 26,31 ; otra causa puede ser la presencia de mecanismos de resistencia diferente a las mutaciones tales como mecanismos de barrera (reducción de la permeabilidad 32-33 y bomba de eflujo [34][35][36][37] ). En cuanto a la presencia de un aislado sensible con la mutación S315T, posiblemente se deba a que el límite de la detección por PCR-RFLP empleada se encuentre cercano al 1% de resistentes que basado en el método de las proporciones pasa imperceptible y se considera como sensible, tambien podría deberse a una inadecuada identificación del perfil de resistencia mediante la prueba de las proporciones en el aislado sensible, y/o contaminación del aislado después de su conservación.…”

Section: Nuestros Resultados Mostraron Que Al Emplear Las Enzimasunclassified

“…La INH actúa inhibiendo la biosíntesis de los ácidos micólicos de la pared celular, e ingresa en el Mycobacterium tuberculosis como un profárma-co por difusión pasiva y es activada por la enzima catalasaperoxidasa codificada por katG, para generar radicales libres, que atacan a continuación múltiples objetivos en las células 8 . Se ha reportado en varios estudios que la resistencia a isoniacida está relacionada con mutaciones específicas en varios genes importantes de Mycobacterium tuberculosis, existiendo variantes genéticas tales como: katG (S315T, S315N, S315D, S315I, S315R, V61G, Q247H, A62T, G383A, C701G, G1388T), inhA (S94A, I21V, I21T, I47T, I194T, 3, 258, 190) y su región promotora (-15 C-T, -8 T-C, T-A ó T-G,-17 G-T), ahpC, nhd, kasA, oxyR, furA, fabG1 [9][10][11][12][13][14][15][16][17][18][19][20][21] , de igual manera, se ha podido mostrar que la aplicación de PCR-RFLP del gen katG permite la identificación eficiente de aislados resistentes a isoniacida pudiendo emplearse como una prueba rápida de detección [22][23][24][25] .…”

Section: Introductionunclassified

Determinación de la mutación S315T del gen katG en aislados resistentes a Isoniacida de Mycobacterium tuberculosis mediante PCR-RFLP

Sánchez-Domínguez

Nicola-Salas

Morey-León

2018

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Cómo citar este artículo: J. Sánchez-Domínguez, et al. Determinación de la mutación S315T del gen katG en aislados resistentes a Isoniacida de Mycobacterium tuberculosis mediante PCR-RFLP. Infectio 2018; 22(4): 178-184 ResumenObjetivo: determinar la mutación S315T del gen katG en aislados de Mycobacterium tuberculosis resistentes a isoniacida mediante la técnica PCR-RFLP. Materiales y métodos: A partir de 68 aislados de Mycobacterium tuberculosis se realizó el análisis de polimorfismo en productos de amplificación de 1054 y 630 pb que contenían la mutación S315T del gen katG mediante PCR-RFLP empleando las enzimas de restricción MspI y SatI. Mediante SPSS se determinó sensibilidad, especificidad, valores predictivo positivo y negativo, coeficientes de probabilidad positivo y negativo. Resultados: El 74,46% de aislados fenotípicamente resistentes y 4,76% fenotípicamente sensibles presentaron la mutación del gen katG S315T. La PCR-RFLP para S315T del gen katG presentó 85,4% de sensibilidad y 95,2% de especificidad con MspI y 85,4% de sensibilidad y 94,4% de especificidad con SatI. Discusión: La PCR-RFLP tiene una alta capacidad resolutiva que depende de la enzima que se emplee como se observó en estudios previos. La presencia de la mutación S315T en pacientes vírgenes al tratamiento sugiere la circulación de aislados resistentes a Isoniacida. Conclusión: La PCR-RFLP resultó una alternativa válida y rápida para el diagnóstico de la resistencia a isoniacida, mediante la detección de la mutación S315T del gen katG en comparación con el método convencional de las proporciones.Palabras Clave: Mutaciones, Polimorfismo, Isoniacida, PCR-RFLP, Mycobacterium tuberculosis, katG. Determination of S315T mutation within the katG gene in isoniazid-resistant Mycobacterium tuberculosis isolate by PCR-RFLP AbstractObjetive: To determine the S315T mutation of the katG gene in Mycobacterium tuberculosis isoniazid-resistant isolates by PCR-RFLP. Materials and Methods: Polymorphism analysis of 1054 and 630 bp products containing the S315T mutation of the katG gene was performed by PCR-RFLP using the MspI and SatI restriction enzymes from 68 Mycobacterium tuberculosis isolates. Sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratio were determined using SPSS. Results: 74.46% of isoniazid-resistant and 4.76% of isoniazid-sensitive isolates showed the S315T mutation in katG gene. The PCR-RFLP for S315T of the katG gene had 85.4% sensitivity and 95.2% specificity with MspI and 85.4% sensitivity and 94.4% specificity with SatI. Discussion: The PCR-RFLP has a high resolutive capacity that depends on the enzyme that is used as it was observed in previous studies. The presence of the S315T mutation in treatment-naive patients suggests the circulation of isolates resistant to isoniazid. Conclusion: PCR-RFLP is a valid and rapid alternative for the diagnosis of isoniazid resistance, by detection of S315T mutation in the katG gene compared to the conventional method of proportions.

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Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar

Abstract: HighlightsDrug-resistant tuberculosis (TB) is a major health threat in Myanmar.The first whole-genome sequencing study of drug-resistant TB from Myanmar.Introduction of second-line drug susceptibility testing as part of routine diagnosis in Myanmar is needed.

Cited by 28 publications

References 15 publications

Dispersal of Mycobacterium tuberculosis Driven by Historical European Trade in the South Pacific

Dispersal of Mycobacterium tuberculosis Driven by Historical European Trade in the South Pacific

Draft Genome Sequences of Two Drug-Resistant Mycobacterium tuberculosis Isolates from Myanmar

Determinación de la mutación S315T del gen katG en aislados resistentes a Isoniacida de Mycobacterium tuberculosis mediante PCR-RFLP

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