2012
DOI: 10.1534/g3.111.001479
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Whole-Genome Sequencing ofSordaria macrosporaMutants Identifies Developmental Genes

Abstract: The study of mutants to elucidate gene functions has a long and successful history; however, to discover causative mutations in mutants that were generated by random mutagenesis often takes years of laboratory work and requires previously generated genetic and/or physical markers, or resources like DNA libraries for complementation. Here, we present an alternative method to identify defective genes in developmental mutants of the filamentous fungus Sordaria macrospora through Illumina/Solexa whole-genome seque… Show more

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Cited by 87 publications
(118 citation statements)
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References 46 publications
(93 reference statements)
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“…Ascospore isolates that carried the hph marker cassette instead of pro45 and that were devoid of the ku70 background were obtained from crosses of primary transformants to a fus spore color mutant (55). Two randomly selected ascospore isolates, N161 (⌬pro45) and R7329 (⌬pro45/fus), were subjected to Southern analysis, confirming the deletion of the pro45 gene (see Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Ascospore isolates that carried the hph marker cassette instead of pro45 and that were devoid of the ku70 background were obtained from crosses of primary transformants to a fus spore color mutant (55). Two randomly selected ascospore isolates, N161 (⌬pro45) and R7329 (⌬pro45/fus), were subjected to Southern analysis, confirming the deletion of the pro45 gene (see Fig.…”
Section: Resultsmentioning
confidence: 99%
“…For S. macrospora , the wild type strain used for RNA-seq was a derivative of the wild type strain used for the original genome sequencing (Nowrousian et al. 2010); however the genome version (v02) used in our analysis is based on corrections of the original genome sequence based on resequencing of the strain also used for RNA-seq (Nowrousian et al. 2012, Teichert et al.…”
Section: Methodsmentioning
confidence: 99%
“…DNA was extracted from 40 sterile progeny as described previously [25]. 40 fertile strains were collected from three crosses of mutants pro30, pro32, and pro34 to fus (Figure S8).…”
Section: Methodsmentioning
confidence: 99%