Whole-genome sequencing of Aspergillus tubingensis G131 and overview of its secondary metabolism potential

Choque, Élodie; Klopp, Christophe; Valière, Sophie; Filho, José Tadeu Raynal Rocha; Mathieu, Florence

doi:10.1186/s12864-018-4574-4

Cited by 28 publications

(32 citation statements)

References 63 publications

(138 reference statements)

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“…A. tubingensis G131 was provided by École Nationale Supérieure Agronomique de Toulouse (France); this fungus is non-mycotoxigenic as the synthetic genes required for the production ochratoxin A and fumonisin are absent from its genome [14]. It was conserved at 4 • C in a 15 mL bottle on potato dextrose agar (PDA).…”

Section: Fungal Materialsmentioning

confidence: 99%

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Carboué¹,

Maresca

Herbette

et al. 2019

Biomolecules

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Seven naphtho-gamma-pyrones (NγPs), including asperpyrone E, aurasperone A, dianhydroaurasperone C, fonsecin, fonsecinone A, fonsecin B, and ustilaginoidin A, were isolated from Aspergillus tubingensis G131, a non-toxigenic strain. The radical scavenging activity of these NγPs was evaluated using ABTS assay. The Trolox equivalent antioxidant capacity on the seven isolated NγPs ranged from 2.4 to 14.6 μmol L−1. The toxicity and ability of the NγPs to prevent H2O2-mediated cell death were evaluated using normal/not cancerous cells (CHO cells). This cell-based assay showed that NγPs: (1) Are not toxic or weakly toxic towards cells and (2) are able to protect cells from oxidant injuries with an IC50 on H2O2-mediated cell death ranging from 2.25 to 1800 μmol mL−1. Our data show that A. tubingensis G131 strain is able to produce various NγPs possessing strong antioxidant activities and low toxicities, making this strain a good candidate for antioxidant applications in food and cosmetic industries.

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Section: Fungal Materialsmentioning

confidence: 99%

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Carboué¹,

Maresca

Herbette

et al. 2019

Biomolecules

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“…In non-ochratoxigenic strains of A. niger, a 21-kb deletion in a remnant of the polyketide sinthase gene of the putative OTA cluster has been identified (Andersen et al, 2011). In the same way, the genome analysis of A. tubingensis revealed that it does not contain an orthologue of this gene (Choque et al, 2018). In previous studies, we resequenced the genome of three atypical and unique non-OTA producing strains of A. carbonarius (Cabañes et al, 2015;Castellá et al, 2018).…”

Section: Introductionmentioning

confidence: 96%

Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

Castellá

Bragulat

Cigliano

et al. 2020

International Journal of Food Microbiology

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Aspergillus carbonarius consistently produces large amounts of ochratoxin A (OTA), a mycotoxin with nephrotoxic effects on animals and humans. In the present study, we analyzed the transcriptional changes associated to OTA production in three atypical non-ochratoxigenic strains of A. carbonarius. In addition, in vitro interactions between ochratoxigenic strains of A. carbonarius and A. niger and non-ochratoxigenic strains of A. carbonarius and A. tubingensis were studied in order to evaluate their potential for controlling OTA production. RNA-seq analysis revealed that there are 696 differentially expressed genes identified in the three non-OTA producing strains, including 280 upregulated and 333 down-regulated genes. A functional and gene ontology enrichment analysis revealed that the processes related to metabolic and oxidation processes, associated with functions such oxidoreductase and hydrolase activity were down regulated. All the genes related with OTA biosynthesis in A. carbonarius were the most down-regulated genes in non-ochratoxigenic strains. We also showed that these strains possess a deleterious mutation in the AcOTApks gene required for OTA biosynthesis. Moreover, one of these strains gave the best control of OTA production resulting in an OTA reduction of 98-100% in co-inoculation with an ochratoxigenic strain of A. niger and an OTA reduction of 79-89% with an ochratoxigenic strain of A. carbonarius. Results of this study provided novel insights into the knowledge of the OTA biosynthetic pathway in these non-ochratoxigenic wild strains, and showed the biocontrol potential of these strains.

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“…The most abundant species isolated from our raisin samples was A. tubingensis, including both samples highly contaminated by OTA (Table 1). Although several authors previously declared the ochratoxigenic potential of A. tubingensis strains [24,28,72], the recent genomic analysis of this species revealed the absence of genes responsible for OTA production [73]. Thus, A. tubingensis cannot be a source of OTA contamination in our dried vine fruit samples.…”

Section: Ochratoxigenic and Fumonisins Production Potential Of Aspergmentioning

confidence: 81%

Genetic Diversity, Ochratoxin A and Fumonisin Profiles of Strains of Aspergillus Section Nigri Isolated from Dried Vine Fruits

Mikušová

Caboň

Melichárková

et al. 2020

Toxins

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We investigated ochratoxin A (OTA) contamination in raisin samples purchased from Slovak markets and determined the diversity of black-spored aspergilli as potential OTA and fumonisin (FB1 and FB2) producers. The taxonomic identification was performed using sequences of the nuclear ITS1-5.8s-ITS2 region, the calmodulin and beta-tubulin genes. We obtained 239 isolates from eight fungal genera, of which 197 belonged to Aspergillus (82%) and 42 strains (18%) to other fungal genera. OTA contamination was evidenced in 75% of the samples and its level ranged from 0.8 to 10.6 µg/kg. The combination of all three markers used enabled unambiguous identification of A. carbonarius, A. luchuensis, A. niger, A. tubingensis and A. welwitschiae. The dominant coloniser, simultaneously having the highest within-species diversity isolated from our raisin samples, was A. tubingensis. Out of all analysed strains, only A. carbonarius was found to produce OTA, but in relatively high quantity (2477–4382 µg/kg). The production of FB1 and FB2 was evidenced in A. niger strains only.

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Whole-genome sequencing of Aspergillus tubingensis G131 and overview of its secondary metabolism potential

Cited by 28 publications

References 63 publications

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

Genetic Diversity, Ochratoxin A and Fumonisin Profiles of Strains of Aspergillus Section Nigri Isolated from Dried Vine Fruits

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