Whole-Genome Sequencing of a Human Clinical Isolate of the Novel Species
            <i>Klebsiella quasivariicola</i>
            sp. nov

Long, S. Wesley; Linson, Sarah E.; Saavedra, Matthew Ojeda; Cantu, Concepcion; Davis, James J.; Brettin, Thomas; Olsen, Randall J.

doi:10.1128/genomea.01057-17

Cited by 48 publications

(45 citation statements)

References 8 publications

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“…quasipneumoniae isolate, but interestingly, isolate 10446 was found to be phylogenetically related to the new bacterial species K. quasivariicola and Klebsiella spp. 10982 (with the last one corresponding to K. quasivariicola ) [5] ( Fig. 1 ).…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Garza–Ramos

Barrios-Camacho

Moreno-Dominguez

et al. 2018

New Microbes and New Infections

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Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013–2014). The biochemical tests identified all as being K. pneumoniae. The molecular multiplex-PCR test identified 36 (92.4%) K. pneumoniae isolates and one (2.5%) K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1%) belonging to K. quasipneumoniae subsp. quasipneumoniae and K. quasivariicola. The last one was confirmed by phylogenetic analysis of six-loci concatenated genes. Mostly the isolates were multidrug resistant; however, a minority were extended-spectrum β-lactamase producing (10.2%). The extended-spectrum β-lactamase CTX-M-15 gene was identified in these isolates. Analysis of biofilm production and the hypermucoviscosity phenotype showed a total of 35 (92.3%) and seven (17.9%) of the isolates were positive for these phenotypes respectively. The K2 (4/39, 10.2%), K5 (2/39, 5.1%) and K54 (1/39, 2.5%) serotypes were identified in seven (17.9%) of the isolates, and only 28.5% (2/7) hypermucoviscous isolates were positive for the K2 and K5 serotypes. In general, the sequence type (ST) analysis and phylogenetic analysis of seven multilocus sequence typing loci were heterogeneous; however, ST29 was the most prevalent ST in the analysed isolates, accounting for 19% (4/21) of the total isolates. Two of the four ST29 isolates had the hypermucoviscosity phenotype. The virulence factors for fimbriae were the most prevalent, followed by siderophores. Community-acquired infections are caused by various species from Klebsiella genus, with different profiles of antibiotic resistance and heterogeneous virulence factors.

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Section: Discussionmentioning

confidence: 99%

“…similipneumoniae (KpII-B), were reported. More recently, a new bacterial species was added to the Klebsiella genus, Klebsiella quasivariicola [4] , [5] . These bacterial species, like K. pneumoniae , are commonly multidrug resistant and can harbour cephalosporinase or carbapenemase genes [1] , [6] .…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Garza–Ramos

Barrios-Camacho

Moreno-Dominguez

et al. 2018

New Microbes and New Infections

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“…variicola subsp. nov. for which the type strain is F2R9 T (= ATCC BAA-830 T , DSM 15968 T ) [6]. In addition to the phenotypic characters consistent within all members of the K. pneumoniae complex, reactions common to both subspecies of K .…”

Section: Resultsmentioning

confidence: 99%

“…similipneumoniae , and K. variicola [4,5]. Phylogenetic analyses of Kp6 genomic sequences showed that this phylogroup corresponds to a recently proposed taxon, ‘ K. quasivariicola’ [6], which remains to be validly published. While screening for K. pneumoniae fecal carriage in Senegal, we identified strain 200023 (internal strain bank identifier, SB5857), which was not associated with any of the previous phylogroups or species, and which we here call Kp7.…”

Section: Introductionmentioning

confidence: 99%

Description ofKlebsiella africanensissp. nov.,Klebsiella variicolasubsp.tropicalensissubsp. nov. andKlebsiella variicolasubsp.variicolasubsp. nov

Rodrigues¹,

Passet²,

Rakotondrasoa

et al. 2019

Preprint

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The bacterial pathogen Klebsiella pneumoniae comprises several phylogenetic groups (Kp1 to Kp7), two of which (Kp5 and Kp7) have no taxonomic status. Here we show that group Kp5 is closely related to Klebsiella variicola (Kp3), with an average nucleotide identity (ANI) of 96.4%, and that group Kp7 has an ANI of 94.7% with Kp1 (K. pneumoniae sensu stricto). Biochemical characteristics and chromosomal beta-lactamase genes also distinguish groups Kp5 and Kp7 from other Klebsiella taxa. We propose the names K. africanensis for Kp7 (type strain, 200023T) and K. variicola subsp. tropicalensis for Kp5 (type strain, 1266T).

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“…Klebsiella pneumoniae strains have the capacity to take up traits (genetic) leading to more virulence and resulting in multidrug resistance. These resistant bacterial strains produce enzymes like cephalosporinase or Carbapenemases that can deactivate antibiotic drug administered on patients (Long et al, 2018), and so integrons, plasmid genes, and transposons harboured by the pathogen induces resistance to antibiotics drugs (Paterson and Bonomo, 2005).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Plasmid Profile of Resistant Klebsiella pneumoniae Isolates in Patients with Urinary Tract Infection in Nasarawa State, Nigeria

Orole

Hadi

2020

Int J Appl Sci Biotechnol

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Klebsiella pneumoniae has been identified as an urgent threat to human health based on its increasing antimicrobial resistance to the beta-lactamases and Carbapenemases. The pathogen has become a threat to both patients and healthcare providers as its incidence is on the increase, becoming a major global healthcare issue. The study was aimed at characterizing and determining the plasmid profile of resistant Klebsiella pneumoniae isolates from urinary tract of patients at Dalhatu Araf Specialist Hospital Lafia, Nasarawa State, Nigeria. Early morning mid-stream urine samples were collected from patients with urinary tract infections between April and May, 2019 and Klebsiella pneumoniae characterized on the basis of its antibiotic resistance pattern, and the plasmid DNA profile determined. Thirty-eight strains of resistant Klebsiella pneumoniae were obtained of which 33 showed resistance to more than three antibiotics. About 51.1% of the isolates were resistant to Tetracycline, while the isolates were least resistant to Azithromycin and Cefotaxime (30.3%) respectively. Klebsiella pneumoniae isolates showed 32 different resistance patterns, 24 of the strains had the capacity to produce Extended Spectrum Beta-lactamases enzymes: CTX-M 24(72.7%), SHV 19(57.6%) and TEM 16(48.5%) respectively. All the resistant Klebsiella pneumoniae isolated had the same plasmid size of 48.5 kilobases and only 1 plasmid each though they all obtained a multiple antibiotic resistance (MAR) index > 0.2. The study concluded that Klebsiella pneumoniae harbours genes which confer antibiotic resistance on the isolates. The study exposes further the challenge of antibiotic resistance and need for concerted effort at stopping the challenge of drug resistance. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 21-28

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Whole-Genome Sequencing of a Human Clinical Isolate of the Novel Species Klebsiella quasivariicola sp. nov

Cited by 48 publications

References 8 publications

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Description ofKlebsiella africanensissp. nov.,Klebsiella variicolasubsp.tropicalensissubsp. nov. andKlebsiella variicolasubsp.variicolasubsp. nov

Characterization and Plasmid Profile of Resistant Klebsiella pneumoniae Isolates in Patients with Urinary Tract Infection in Nasarawa State, Nigeria

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