Whole-Genome Sequencing and Potassium-Solubilizing Mechanism of Bacillus aryabhattai SK1-7

Abstract

To analyze the whole genome of Bacillus aryabhattai strain SK1-7 and explore its potassium solubilization characteristics and mechanism, thus providing a theoretical basis for analyzing the utilization and improvement of insoluble potassium resources in soil. Genome information for Bacillus aryabhattai SK1-7 was obtained by using Illumina NovaSeq second-generation sequencing and GridION Nanopore ONT third-generation sequencing technology. The contents of organic acids and polysaccharides in fermentation broth of Bacillus aryabhattai SK1-7 were determined by high-performance liquid chromatography and the anthrone sulfuric acid method, and the expression levels of the potassium solubilization-related genes ackA, epsB, gltA, mdh and ppc were compared by real-time fluorescence quantitative PCR under different potassium source culture conditions. The whole genome of the strain consisted of a complete chromosome sequence and four plasmid sequences. The sequence sizes of the chromosomes and plasmids P1, P2, P3 and P4 were 5,188,391 bp, 136,204 bp, 124,862 bp, 67,200 bp and 12,374 bp, respectively. The GC contents were 38.2, 34.4, 33.6, 32.8, and 33.7%. Strain SK1-7 mainly secreted malic, formic, acetic and citric acids under culture with an insoluble potassium source. The polysaccharide content produced with an insoluble potassium source was higher than that with a soluble potassium source. The expression levels of five potassium solubilization-related genes with the insoluble potassium source were higher than those with the soluble potassium source.

Transcriptomic Analysis Reveals the Response of the Bacterium Priestia Aryabhattai SK1-7 to Interactions and Dissolution with Potassium Feldspar

“…4 ). Previous studies in our laboratory showed that the aluminum and silica ion levels in the fermentation filtrate of strain SK1-7 in FM (feldspar) showed a gentle increasing trend for the first 7 days ( 34 ). It is assumed that the gradual shift to acidic conditions in the medium resulted in the release of Al 3+ , Si 4+ , etc., from potassium feldspar.…”

“…This bacterium can effectively dissolve insoluble potassium and release soluble potassium ions, with a release rate of 32.6%. The pH of SK1-7 under incubation with an insoluble potassium source decreases rapidly in a relatively short period of time, with the strain mainly secreting malic acid, formic acid, acetic acid, and citric acid, thereby releasing potassium, silicon, and aluminum ions from potassium feldspar ( 34 ). Gupta et al ( 35 ) divided Bacillus species into 17 different branches by phylogenomic and comparative genomic analyses, and B. aryabhattai was renamed Priestia aryabhattai .…”

Overview of biofertilizers in crop production and stress management for sustainable agriculture

“…Enterococcus and Pseudomonas aeruginosa also showed P and K solubilization and improved the maize height, yield, and nutrient acquisition (Kumar et al, 2021b ). Bacillus aryabhattai showed K solubilization, protects plants from stress, and improves their growth via the expression of K-solubilizing genes (Chen et al, 2022 ).…”

Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method

“…that can be hydrolyzed into reducing sugars under the measurement conditions ( 11 , 12 ). The determination methods of total sugar content mainly include phenol-sulfuric acid method ( 2 ), anthrone-sulfuric acid method ( 13 ), full-wavelength enzyme labeling method ( 14 ), and direct titration method ( 15 ). In the current research, due to the simple detection method, good stability and high sensitivity, phenol-sulfuric acid method is widely used ( 16 ).…”