Whole-genome sequencing and antimicrobial resistance analysis of multidrug-resistant Aeromonas veronii strain JC529 from a common carp

Sheng, Tiange; Song, G. S.; Yue, Taotao; Zhang, Junhui; Wang, Wendong; Yang, Zhen-Guo; Lü, Qiang

doi:10.1016/j.jgar.2021.08.007

Cited by 15 publications

(10 citation statements)

References 33 publications

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“…Both phenotypic and genotypic data revealed that the Alim_AV_1000 strain is a multidrug-resistant strain. Similar to our study, multidrug-resistant A. veronii was isolated in India and showed resistance to several antibiotics such as ampicillin, ciprofloxacin, colistin, and trimethoprim [ 21 ]. Moreover, river water isolates of A. veronii in Italy also showed resistance to various antimicrobial agents, such as levofloxacin, colistin, and pipercillin [ 22 ].…”

Section: Discussionsupporting

confidence: 79%

Complete genome sequence analysis of the multidrug resistant Aeromonas veronii isolated for the first time from stinging catfish (Shing fish) in Bangladesh

Rahman,

Sadekuzzaman,

Rahman

et al. 2023

J Adv Vet Anim Res

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Objective: Whole genome sequencing (WGS) of Aeromonas veronii Alim_AV_1000 isolated from ulcerative lesions of Shing fish (stringing catfish; Heteropneustes fossilis) was performed during the outbreak year 2021. Materials and Methods: Using next-generation sequencing (Illumina) technology, WGS was accomplished, resulting in the sequencing, assembly, and analysis of the entire genome of the A. veronii strain. Moreover, the genomic features, virulence factors, antimicrobial resistome, and phylogenetic analysis for the molecular evolution of this strain were also examined. Results: The genome size of the A. veronii Alim_AV_1000 strain was 4,494,515 bp, with an average G+C content of 58.87%. Annotation revealed the known transporters and genes linked to virulence, drug targets, and antimicrobial resistance. Conclusion: The findings of the phylogenetic analysis revealed that the strain of the present study has a close relationship with the China strain TH0426 and strain B56. This study provides novel information on A. veronii isolated from Shing fish in Bangladesh.

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Section: Discussionsupporting

confidence: 79%

Complete genome sequence analysis of the multidrug resistant Aeromonas veronii isolated for the first time from stinging catfish (Shing fish) in Bangladesh

Rahman,

Sadekuzzaman,

Rahman

et al. 2023

J Adv Vet Anim Res

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“…Some of the efflux pump showed resistance activity in response to pathogenic bacterium that have been previously reported to P. koreensis CM-01 (Kho et al, 2023) and A. veronii JC529 (Sheng et al, 2021). AcrAB-TolC is a resistance-nodulation-cell division (RND) multiple drug efflux pump complex and it mediates the resistance against fluoroquinolone and tetracycline antibiotics, which is essential for intrinsic bacterial antibiotic resistance (Andersen et al, 2015;Hocquet et al, 2003;Li et al, 2015;Masuda et al, 2000;Swick et al, 2011).…”

Section: Discussionmentioning

confidence: 92%

Phenotypic and genomic insights into the pathogenicity and antimicrobial resistance of an Enterobacter roggenkampii strain isolated from diseased silver arowana (Osteoglossum bicirrhosum)

Li,

Sun

2023

Journal of Fish Diseases

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Enterobacter roggenkampii is an opportunistic pathogen that causes infections in a wide range of hosts. A bacterial strain named EOBSR_19 was isolated from diseased silver arowana, Osteoglossum bicirrhosum. This bacterium was identified as E. roggenkampii based on the phenotypic characteristics and sequence analysis of the16S rDNA and gyrB genes. Average nucleotide identity and phylogenetic analysis based on the whole genome sequence further confirmed the bacterial taxonomy of EOBSR_19. Artificial experimental infection indicated that EOBSR_19 was pathogenic to fish. Antimicrobial susceptibility test showed it was multi‐drug resistant. The EOBSR_19 was found to be resistant to 18 antibiotics belonging to quinolones, macrolides, sulfonamides, aminoglycosides, and β‐lactams classes. The whole genome sequencing analysis showed that EOBSR_19 carried 730 virulence genes that were annotated for different functional modules, such as adhesion and invasion, secretion system, siderophore transport system and bacterial toxin. Among them, the virulence genes related to adhesion and invasion were the most abundant. In addition, drug resistance genes involved in multiple mechanisms of antimicrobial resistance were identified in its genomics, including multidrug resistance efflux pumps, antibiotic inactivating enzymes, and antibiotic binding site mutations. Its genomic analysis via whole‐genome sequencing provided insights into the pathogenicity and antimicrobial resistance.

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“…After isolating and identifying the pathogen and the regression infection we found that the channel catfish's hemorrhagic disease pathogen was A. veronii. This pathogen mainly infects freshwater fish, such as carp [17]. Infections with A. veronii can cause septicemia, bleeding, ulcer, ascites, and other symptoms, leading to high mortality and morbidity and bringing substantial economic losses to aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Pathogenicity of Aeromonas veronii, the Causal Agent of Hemorrhagic Septicemia in Channel Catfish (Ictalurus punctatus) in China

Qin

et al. 2022

Fishes

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Herein, we isolated the pathogenic strain ZZ051 from hemorrhagic channel catfish (Ictalurus punctatus). Physiological and biochemical identification, 16S rRNA gene sequence analysis, and MALDI-TOF-MS showed that the ZZ051 strain was Aeromonas veronii. After artificial infection, the diseased fish showed symptoms similar to the natural disease, and the characteristics of the bacteria reisolated from the tissues were the same as those of the original infection, indicating that the isolated strain ZZ051 was the pathogen responsible for the channel catfish disease. The ZZ051 isolate was highly sensitive to enrofloxacin but resistant to florfenicol. This study provided a theoretical basis for preventing and controlling hemorrhagic disease in channel catfish.

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Whole-genome sequencing and antimicrobial resistance analysis of multidrug-resistant Aeromonas veronii strain JC529 from a common carp

Cited by 15 publications

References 33 publications

Complete genome sequence analysis of the multidrug resistant Aeromonas veronii isolated for the first time from stinging catfish (Shing fish) in Bangladesh

Complete genome sequence analysis of the multidrug resistant Aeromonas veronii isolated for the first time from stinging catfish (Shing fish) in Bangladesh

Phenotypic and genomic insights into the pathogenicity and antimicrobial resistance of an Enterobacter roggenkampii strain isolated from diseased silver arowana (Osteoglossum bicirrhosum)

Isolation, Identification, and Pathogenicity of Aeromonas veronii, the Causal Agent of Hemorrhagic Septicemia in Channel Catfish (Ictalurus punctatus) in China

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