Whole-Genome Sequences of Two NDM-1-Producing Pseudomonas aeruginosa Strains Isolated in a Clinical Setting in Albania in 2018

Tafaj, Silva; Gona, Floriana; Rodrigues, Célia Fortuna; Kapisyzi, Perlat; Caushi, Fatmir; Rossen, John W. A.; Cirillo, Daniela María

doi:10.1128/mra.01291-19

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…It should be mentioned that this is the second study form the Balkans that utilizes ONT long reads (MinION) in combination with the short reads generated by an Illumina platform (Illumina HiSeq, 2 × 150 bp) [ 131 ]. This results in the generation of the second complete genome of a CRPA isolate again carrying the bla NDM-1 gene similar to the report from Bulgaria [ 127 ]. The absence of essential clinical information regarding the isolates that were subject to WGS can be considered as the biggest limitation of this study.…”

Section: P Aeruginosasupporting

confidence: 73%

“…In their study, Tafaj et al report the genome sequences of two NDM-1-producing P. aeruginosa strains of ST235 that were isolated from the surgical wound of two inpatients in Tirana [ 127 ]. The WGS procedure was carried out at the San Raffaele Hospital (Milan, Italy) using Illumina NextSeq 500 (2 × 150 bp) sequencing.…”

Section: P Aeruginosamentioning

confidence: 99%

“…It generated two draft genomes, each composed by more than 500 contigs. Both of them were found to harbor bla NDM-1 genes in combination with a total of 58 other AMR genetic determinants, including genes conferring resistance to β-lactams, aminoglycosides, fluoroquinolones, macrolides, and tetracyclines, through different mechanisms, such as antibiotic efflux ( n = 37), antibiotic efflux and antibiotic target alteration ( n = 3), antibiotic inactivation ( n = 11), antibiotic target alteration ( n = 6), and antibiotic target replacement ( n = 1) [ 127 ]. All of them were identified using the Resistance Gene Identifier (RGI) v.5.1.0 from the Comprehensive Antibiotic Resistance Database (CARD) [ 133 ].…”

Section: P Aeruginosamentioning

confidence: 99%

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Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Peykov

2023

Microorganisms

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Non-fermenting Gram-negative bacilli (NFGNB), such as Pseudomonas aeruginosa and Acinetobacter baumannii, are among the major opportunistic pathogens involved in the global antibiotic resistance epidemic. They are designated as urgent/serious threats by the Centers for Disease Control and Prevention and are part of the World Health Organization’s list of critical priority pathogens. Also, Stenotrophomonas maltophilia is increasingly recognized as an emerging cause for healthcare-associated infections in intensive care units, life-threatening diseases in immunocompromised patients, and severe pulmonary infections in cystic fibrosis and COVID-19 individuals. The last annual report of the ECDC showed drastic differences in the proportions of NFGNB with resistance towards key antibiotics in different European Union/European Economic Area countries. The data for the Balkans are of particular concern, indicating more than 80% and 30% of invasive Acinetobacter spp. and P. aeruginosa isolates, respectively, to be carbapenem-resistant. Moreover, multidrug-resistant and extensively drug-resistant S. maltophilia from the region have been recently reported. The current situation in the Balkans includes a migrant crisis and reshaping of the Schengen Area border. This results in collision of diverse human populations subjected to different protocols for antimicrobial stewardship and infection control. The present review article summarizes the findings of whole-genome sequencing-based resistome analyses of nosocomial multidrug-resistant NFGNBs in the Balkan countries.

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Section: P Aeruginosasupporting

confidence: 73%

Section: P Aeruginosamentioning

confidence: 99%

Section: P Aeruginosamentioning

confidence: 99%

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Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Peykov

2023

Microorganisms

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“…Several recent studies also supported that a longer hospital stay increases the risk of AB infection. [ 23 , 24 ] We found that the absence of coinfection to be an additional risk factor. Consistent with our results, the coinfection of other bacteria was rare in AB-infected patients.…”

Section: Discussionmentioning

confidence: 85%

Risk factors for pneumonia caused by antimicrobial drug-resistant or drug-sensitive Acinetobacter baumannii infections

Hu²

2020

Medicine

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Acinetobacter baumannii (AB) is one of the major types of infection in hospitalized patients. The development of AB resistance is becoming a global clinical challenge. To assist in the clinical management of AB-induced pneumonia, we designed the present retrospective observational study to investigate the risk factors for antimicrobial drug-resistant/-sensitive AB infections. A total of 214 individuals were reviewed, in which 100 and 55 pneumonia patients were infected with drug-resistant and drug-sensitive AB, respectively. Fifty-nine pneumonia patients without AB infection served as a control group. Age, sex, duration of hospital stay, prior surgery history, the presence of coinfection and companion diseases, routine blood test results, and immunogenicity were recorded. Logistic regression was performed to identify risk factors of AB infections. Multivariate analysis revealed that long duration of hospital stay (odds ratio = 1.091 [95% CI: 1.010–1.178], P = .027) and the absence of coinfection (odds ratio = 0.507 [95% CI: 0.265–0.970], P = .040) were independent risk factors for AB infections. Same pattern of risk factors was identified for the drug-sensitive group (long duration of hospital stay: odds ratio = 1.119 [95% CI: 1.016–1.232], P = .022; absence of coinfection: odds ratio = 0.328 [95% CI: 0.135–0.797], P = .014), while high blood urea nitrogen (odds ratio: 1.382 [95% CI: 1.042–1.833], P = .025) was the only significant risk factor for drug-resistant AB infection. Long duration of hospital stay and the absence of coinfection might predict AB infections in hospitalized patients. Antimicrobial drug-resistant and drug-sensitive AB infections possess different risk factor profiles. A poor kidney function may be predictive of drug-resistant AB infection. Further prospective studies are required to validate our findings.

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“…Since the first discovery of NDM-1-producing P. aeruginosa from Serbia in 2011 [8], it has been isolated mostly in Asia, Europe, and Africa [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] (Table 1). ST235 was the only sequence type identified in all previously isolated NDM-1-producing P. aeruginosa strains.…”

Section: Discussionmentioning

confidence: 99%

Detection of NDM-1 Metallo-β-Lactamase Genes in Pseudomonas Aeruginosa from Clinical Surveillance Sample: A Case Report and Literature Review

Chang

Kim

Kwak

et al. 2021

Korean J healthc assoc Infect Control Prev

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Infections caused by gram-negative organisms are a global concern [1,2]. Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa are major pathogens that can cause healthcare-associated infections, especially if the organisms are carbapenem resistant [3,4]. P. aeruginosa is an opportunistic pathogen that can cause severe infections, such as respiratory tract infec-tions, sepsis, and urinary tract infections, particularly in immunocompromised patients. Moreover, it is an important nosocomial pathogen [5]. In Korea, there have been several reports of metallo-β-lactamase (MBL)-producing P. aeruginosa, including those that produce Verona integron-encoded metallo-β-lactamase (VIM) and active-onimipenem (IMP) [6], but reports on New Delhi metalloβ-lactamase 1 (NDM-1) are rare. It is important to detect MBL-producing organisms early because these organ-

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Whole-Genome Sequences of Two NDM-1-Producing Pseudomonas aeruginosa Strains Isolated in a Clinical Setting in Albania in 2018

Cited by 5 publications

References 10 publications

Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Risk factors for pneumonia caused by antimicrobial drug-resistant or drug-sensitive Acinetobacter baumannii infections

Detection of NDM-1 Metallo-β-Lactamase Genes in Pseudomonas Aeruginosa from Clinical Surveillance Sample: A Case Report and Literature Review

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