Whole-Genome Sequences of Pantoea agglomerans BL3, Pseudomonas fluorescens BL, and Pseudomonas stutzeri CM14, Isolated from Hops (Humulus lupulus)

Sevigny, Joseph L.; Lloyd, Breana; McComish, Catherine; Ramsey, Alexis; Koziol, Lori

doi:10.1128/mra.00545-19

Cited by 6 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The untreated Trifolium pratense seedlings did not show the internal presence of the tagged bacteria ( Figure 15 A). Sevigny et al [ 84 ] isolated pseudomonads ( Pseudomonas fluorescens and Pseudomonas stutzeri ) from Humulus lupulus flowers (cones) and found the presence of nitrogenase genes in their genomes. We also isolated potential nitrogen-fixing species of Massilia and Pantoea from washes from Humulus lupulus cones ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

Histochemical Evidence for Nitrogen-Transfer Endosymbiosis in Non-Photosynthetic Cells of Leaves and Inflorescence Bracts of Angiosperms

Micci

Zhang

Chang

et al. 2022

Biology

View full text Add to dashboard Cite

We used light and confocal microscopy to visualize bacteria in leaf and bract cells of more than 30 species in 18 families of seed plants. Through histochemical analysis, we detected hormones (including ethylene and nitric oxide), superoxide, and nitrogenous chemicals (including nitric oxide and nitrate) around bacteria within plant cells. Bacteria were observed in epidermal cells, various filamentous and glandular trichomes, and other non-photosynthetic cells. Most notably, bacteria showing nitrate formation based on histochemical staining were present in glandular trichomes of some dicots (e.g., Humulus lupulus and Cannabis sativa). Glandular trichome chemistry is hypothesized to function to scavenge oxygen around bacteria and reduce oxidative damage to intracellular bacterial cells. Experiments to assess the differential absorption of isotopic nitrogen into plants suggest the assimilation of nitrogen into actively growing tissues of plants, where bacteria are most active and carbohydrates are more available. The leaf and bract cell endosymbiosis types outlined in this paper have not been previously reported and may be important in facilitating plant growth, development, oxidative stress resistance, and nutrient absorption into plants. It is unknown whether leaf and bract cell endosymbioses are significant in increasing the nitrogen content of plants. From the experiments that we conducted, it is impossible to know whether plant trichomes evolved specifically as organs for nitrogen fixation or if, instead, trichomes are structures in which bacteria easily colonize and where some casual nitrogen transfer may occur between bacteria and plant cells. It is likely that the endosymbioses seen in leaves and bracts are less efficient than those of root nodules of legumes in similar plants. However, the presence of endosymbioses that yield nitrate in plants could confer a reduced need for soil nitrogen and constitute increased nitrogen-use efficiency, even if the actual amount of nitrogen transferred to plant cells is small. More research is needed to evaluate the importance of nitrogen transfer within leaf and bract cells of plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Histochemical Evidence for Nitrogen-Transfer Endosymbiosis in Non-Photosynthetic Cells of Leaves and Inflorescence Bracts of Angiosperms

Micci

Zhang

Chang

et al. 2022

Biology

View full text Add to dashboard Cite

show abstract

“…Recently, P. agglomerans was present among three species, which have been isolated from hop cones (Sevigny et al . 2019).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification ofPantoea agglomeransfrom the inflated bag with dried hop pellets stored under a modified atmosphere

Kolek

Patáková

Junková

et al. 2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

Aims Isolation, characterization and identification of possible microbial contaminant(s) in the inflated foil bag containing hop pellets packed and stored in a modified atmosphere. Methods and results Package gas of the inflated foil bag containing hop pellets was analysed by gas chromatography. Compared with the reference modified atmosphere, containing about 16 vol.% of CO2, the inflated bag atmosphere contained 53 vol.% CO2, suggesting possible microbial contamination. Therefore, several standard and mineral media, with added hop pellets or hop infusion, were used for cultivation at different temperatures under an anaerobic atmosphere. Cultivation in mineral medium with hop pellets yielded a bacterial isolate that was identified by MALDI‐TOF mass spectrometry and verified by partial 16S rRNA gene analysis as Pantoea agglomerans, a known plant epiphyte. Conclusions A novel strain of P. agglomerans (designed as DBM 3696) was found to be suspicious of causing inflation of the foil bag containing dried hop pellets packed in modified atmosphere. Significance and Impact of the Study This study suggests that P. agglomerans, probably hop epiphyte, could cause sporadic inflation of bags with hop pellets packed in modified atmosphere causing logistical problems during bags transport.

show abstract

“…The considerable resistance of hops to bacteria is also evidenced by the fact that bacterial diseases of hops are rare compared to viruses or diseases caused by fungi. Nevertheless, some bacteria have already been isolated from hops—bacteria of the genus Streptomyces from the rhizosphere of hops (Koçak, 2019 ), Pseudomonas stutzeri and Pseudomonas fluorescens from hop cones (Sevigny et al, 2019 ) and Pantoea agglomerans from hop cones (Sevigny et al, 2019 ) and from dried hop pellets (Kolek et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and characterization of Pantoea agglomerans DBM 3797, endophyte, isolated from fresh hop (Humulus lupulus L.)

Patakova,

Vasylkivska,

Sedlar

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

BackgroundThis paper brings new information about the genome and phenotypic characteristics of Pantoea agglomerans strain DBM 3797, isolated from fresh Czech hop (Humulus lupulus) in the Saaz hop-growing region. Although P. agglomerans strains are frequently isolated from different materials, there are not usually thoroughly characterized even if they have versatile metabolism and those isolated from plants may have a considerable potential for application in agriculture as a support culture for plant growth.MethodsP. agglomerans DBM 3797 was cultured under aerobic and anaerobic conditions, its metabolites were analyzed by HPLC and it was tested for plant growth promotion abilities, such as phosphate solubilization, siderophore and indol-3-acetic acid productions. In addition, genomic DNA was extracted, sequenced and de novo assembly was performed. Further, genome annotation, pan-genome analysis and selected genome analyses, such as CRISPR arrays detection, antibiotic resistance and secondary metabolite genes identification were carried out.Results and discussionThe typical appearance characteristics of the strain include the formation of symplasmata in submerged liquid culture and the formation of pale yellow colonies on agar. The genetic information of the strain (in total 4.8 Mb) is divided between a chromosome and two plasmids. The strain lacks any CRISPR-Cas system but is equipped with four restriction-modification systems. The phenotypic analysis focused on growth under both aerobic and anaerobic conditions, as well as traits associated with plant growth promotion. At both levels (genomic and phenotypic), the production of siderophores, indoleacetic acid-derived growth promoters, gluconic acid, and enzyme activities related to the degradation of complex organic compounds were found. Extracellular gluconic acid production under aerobic conditions (up to 8 g/l) is probably the result of glucose oxidation by the membrane-bound pyrroloquinoline quinone-dependent enzyme glucose dehydrogenase. The strain has a number of properties potentially beneficial to the hop plant and its closest relatives include the strains also isolated from the aerial parts of plants, yet its safety profile needs to be addressed in follow-up research.

show abstract

Whole-Genome Sequences of Pantoea agglomerans BL3, Pseudomonas fluorescens BL, and Pseudomonas stutzeri CM14, Isolated from Hops (Humulus lupulus)

Abstract: Pseudomonas stutzeri CM14, Pseudomonas fluorescens BL, and Pantoea agglomerans BL3 were isolated from Humulus lupulus cones. Here, we present the draft genome sequences of three bacteria that have been associated with hop plants.

Cited by 6 publications

References 10 publications

Histochemical Evidence for Nitrogen-Transfer Endosymbiosis in Non-Photosynthetic Cells of Leaves and Inflorescence Bracts of Angiosperms

Histochemical Evidence for Nitrogen-Transfer Endosymbiosis in Non-Photosynthetic Cells of Leaves and Inflorescence Bracts of Angiosperms

Isolation and identification ofPantoea agglomeransfrom the inflated bag with dried hop pellets stored under a modified atmosphere

Whole genome sequencing and characterization of Pantoea agglomerans DBM 3797, endophyte, isolated from fresh hop (Humulus lupulus L.)

Contact Info

Product

Resources

About