Whole genome sequence resource of Indian Zaprionus indianus

Abstract

This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first Indian Z. indianus whole genome (draft) submitted to the sequence repository with SRA reads. The details of methodology, assembly statistics and functional annotation are presented in this work.

Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid <b><i>Zaprionus indianus</i></b>

“…The 12F6 antibody was generated originally to Hyalophora cecropia hemocytes, and cross-reacts with the crystal cells of D. melanogaster, Manduca sexta [31,32] and Z. indianus blood cells. In D. melanogaster, this antibody reacts with the prophenoloxydase PPO2 (79.3 kDa) [32], which shows an 88% homology on a 645 amino acid stretch with a protein encoded by a region of the LWKS01004021.1 Z. indianus strain IND_ZI_P10 Scaffold4157 whole-genome shotgun sequence [29]. As the predicted molecular mass of the PPO2 Z. indianus ortholog was 75 kDa, corresponding to the band marked by the 12F6 antibody in the Western blot analysis of Z. indianus hemocytes (online suppl.…”

“…While the species was originally prevalent in the Oriental region and Africa [25], it appeared in Brazil in 1999 [26], spreading rapidly throughout all of South America, as well as parts of the North American continent [27]. Recently, it was found in France [28], and it is therefore expected that within a short period of time it could cause economic problems in Europe as well [29]. Therefore, it is of great interest to learn about the biology and the immune defense mechanisms of this species, with special emphasis on the MGHs.…”

Genomic signatures of admixture and selection are shared among populations of Zaprionus indianus across the western hemisphere

“…The sequence for Z . indianus from India was generated from two males from a single isofemale line that was collected in Punjab, India (Khanna & Mohanty, 2017 ).…”

“…Zaprionus gabonicus and Z. africanus are the two most closely related species to Z. indianus, and each of these species are reproductively isolated from one another (Yassin et al, 2008). A total of 56 of the genomes in our data set were previously analysed to study levels of genetic diversity across different populations and species of Zaprionus (Comeault et al, 2020), and one is from publicly available data used to generate a draft genome assembly for Z. indianus collected in India (Khanna & Mohanty, 2017). Here we add four genome sequences from a population in South America (Medellín, Colombia) and 14 genomes from five locations in the eastern United States (Florida, Pennsylvania, New Jersey, New York, and North Carolina; see Table S1).…”

“…Individuals were sequenced to a mean depth of 20-49× using paired-end Illumina reads as described in Comeault et al (2020). The sequence for Z. indianus from India was generated from two males from a single isofemale line that was collected in Punjab, India (Khanna & Mohanty, 2017).…”