Whole-Genome Sequence of Staphylococcus hominis, an Opportunistic Pathogen

Jiang, Saiping; Zheng, Beiwen; Ding, Wenchao; Lv, Longxian; Ji, Jinru; Xiao, Yonghong; Li, Lanjuan

doi:10.1128/jb.00991-12

Cited by 28 publications

(20 citation statements)

References 14 publications

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“…The most numerous species in the analyzed material was S. hominis (18%), which is usually isolated from human skin armpits, arms, legs and trunk. S. hominis usually does not cause serious infections in humans, but it is an opportunistic pathogen that can contribute to infections of hospitalized patients weakened by long-term pharmacological treatment (Jiang et al 2012 ). On the other hand, studies conducted by Pastuszka et al ( 2000 ) in flats of Upper Silesia (Poland) showed that the main bacterial species isolated from indoor air was S. epidermidis , which was present in 76% of studied flats constituting 14% of the total amount of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular assessment of antimicrobial resistance profile of airborne Staphylococcus spp. isolated from flats in Kraków

et al. 2017

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Bacteria of the genus Staphylococcus were isolated from air sampled from living spaces in Kraków (Poland). In total, 55 strains belonging to the genus Staphylococcus were isolated from 45 sites, and 13 species of coagulase-negative staphylococci were identified. The species composition of studied airborne microbiota contains Staphylococcus species that are rarely infectious to humans. Most commonly isolated species comprised S. hominis and S. warneri. The disk-diffusion tests showed that the collected isolates were most frequently resistant to erythromycin. The PCR technique was employed to search for genes conferring the resistance in staphylococci to antibiotics from the group of macrolides, lincosamides and streptogramins. The analyzed Staphylococcus isolates possessed simultaneously 4 different resistance genes. The molecular analysis with the use of specific primers allowed to determine the most prevalent gene which is mphC, responsible for the resistance to macrolides and for the enzymatic inactivation of the drug by phosphotransferase. The second most often detected gene was msrA1, which confers the resistance of staphylococci to macrolides and is responsible for active pumping of antimicrobial particles out of bacterial cells.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular assessment of antimicrobial resistance profile of airborne Staphylococcus spp. isolated from flats in Kraków

et al. 2017

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“…All the pure cultures were frozen at -80°C and shipped to our laboratory for definite identification and further analysis. The identities of all isolates were ratified using Matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF) as described previously [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Nationwide high prevalence of CTX-M and an increase of CTX-M-55 in Escherichia coli isolated from patients with community-onset infections in Chinese county hospitals

et al. 2014

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BackgroundIn order to investigate the epidemiology, molecular characteristics, and distribution of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli from community-onset infections in Chinese county hospitals.MethodsE. coli isolates were collected from patients with community-onset infections in 30 county hospitals. ESBL activity was confirmed by double-disc diffusion. Genetic confirmation and molecular typing of ESBL- and AmpC-producing isolates was determined by PCR and DNA sequencing. ESBL-positive isolates were further characterised by multi-locus sequence typing.ResultsOf 550 E. coli isolates, 256 (46.5%) carried ESBL genes and all were of the CTX-M type. The prevalence of ESBL-producing strains varied from 30.2% to 57.0% across different regions of China. Overall, 12 blaCTX-M subtypes were detected; the most abundant were blaCTX-M-14 (163/256 isolates, 64.5%), blaCTX-M-55(47/256, 18.4%), and blaCTX-M-15 (31/256, 12.1%). CMY-2-like AmpC β-lactamases were detected in 11 strains, three of which co-existed with blaCTX-M. A total of 64 sequence types (STs) were detected in 256 ESBL-producing strains, including nine that were new. ST131 was the most abundant type (27 isolates, 12.7%), followed by ST69 (14 isolates, 6.6%), ST405 (14 isolates, 6.6%), and ST38 (12 isolates, 5.6%).ConclusionsThis study revealed that the widespread prevalence of ESBLs among outpatient infections has reached a high level in county hospitals. The CTX-M genotype was most dominant, comprising a variety of subtypes. This is the first time the incidence of CTX-M-55 has exceeded that of CTX-M-15 in China. No predominant ST was detected, suggesting that ESBL-producing E. coli strains originate in different clones.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-014-0659-0) contains supplementary material, which is available to authorized users.

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“…The genome contains 58 tRNA genes coding for all amino acids and 2,248 predicted protein-coding genes, consistent with other staphylococci (3,5). We identified numerous putative virulence factors.…”

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confidence: 83%

Genome Sequence of Staphylococcus epidermidis Strain AU12-03, Isolated from an Intravascular Catheter

et al. 2012

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bIn recent years, Staphylococcus epidermidis has become a major nosocomial pathogen and the most common cause of intravascular catheter-related bacteremia, which can increase morbidity and mortality and significantly affect patient recovery. We report a draft genome sequence of Staphylococcus epidermidis AU12-03, isolated from an intravascular catheter tip.T he staphylococci are able to cause a variety of infections ranging from minor skin infections to life-threatening bacteremia, and in spite of great efforts to control their spread, they persist as a major cause of hospital-acquired infections worldwide (4). Staphylococcus epidermidis is a member of the staphylococci and has become the leading cause of infection related to indwelling medical devices, such as intravascular catheters, prosthetic joints, and artificial heart valves (2). Previous reports have identified S. epidermidis as having the ability to colonize medical device surfaces, produce antimicrobials, and form biofilms (2, 8).S. epidermidis AU12-03 was isolated from an intravascular catheter tip by rolling a catheter tip back and forth on the surface of a Columbia agar plate supplemented with 5% sheep blood essentially as described by Maki et al. (6). The genome sequence of S. epidermidis AU12-03 was determined on a 454 GS FLX system (Roche) (7). The sequence data consist of 125,924,967 bp of DNA sequence at 51ϫ coverage. A total of 50 contigs (Ͼ500 bp) were de novo assembled using the GS De Novo assembler (version 2.3; Roche). The contig N 50 was 81,766 bp, and the largest contig assembled was 210,685 bp. The contigs were then ordered and oriented into nine scaffolds using paired-end information. The average length of the scaffolds was 271,213 bp. Automatic genome annotation was performed on the RAST server (1).The draft genome of S. epidermidis AU12-03 consists of a circular 2,440,923-bp chromosome with a GϩC content of 31.98%. The genome contains 58 tRNA genes coding for all amino acids and 2,248 predicted protein-coding genes, consistent with other staphylococci (3, 5). We identified numerous putative virulence factors. S. epidermidis AU12-03 carried some predicted proteincoding genes involved in adhesion (cell wall-associated fibronectin-binding protein, elastin-binding protein, the bifunctional autolysin Atl, and the virulence-associated cell wall-anchored protein LPXTG motif binding to squamous nasal epithelial cells) and regulators involved in quorum sensing and biofilm formation (the staphylococcal accessory regulator SarA, the RNA polymerase sigma factor SigB, the bifunctional autolysin Atl, the antiadhesin Pls, the teicoplanin resistance-associated HTH-type transcriptional regulator TcaR, and the transcriptional regulator of biofilm formation AraC/XylS family). A range of genes related to bacteriocins and invasion were found. As expected, we also identified putative antibiotic resistance determinants (resistance to fluoroquinolone and beta-lactam antibiotics).In short, the genomic information suggests that S. epidermidis AU12-03 may form...

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Whole-Genome Sequence of Staphylococcus hominis, an Opportunistic Pathogen

Cited by 28 publications

References 14 publications

Phenotypic and molecular assessment of antimicrobial resistance profile of airborne Staphylococcus spp. isolated from flats in Kraków

Phenotypic and molecular assessment of antimicrobial resistance profile of airborne Staphylococcus spp. isolated from flats in Kraków

Nationwide high prevalence of CTX-M and an increase of CTX-M-55 in Escherichia coli isolated from patients with community-onset infections in Chinese county hospitals

Genome Sequence of Staphylococcus epidermidis Strain AU12-03, Isolated from an Intravascular Catheter

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