Whole genome sequence of Pantoea agglomerans ANP8, a salinity and drought stress–resistant bacterium isolated from alfalfa (Medicago sativa L.) root nodules

Abstract

Highlights Strain ANP8 was isolated from root nodules of alfalfa grown on saline soil. ANP8 has multiple PGP traits and is resistant to salinity and drought stress. Complete genome sequence of Pantoea agglomerans ANP8 was presented. The growth rate of ANP8 was considerable compared to the growth rate of E. coli .

Opposite Sides of Pantoea agglomerans and Its Associated Commercial Outlook

“…Genes related to the direct mechanisms of plant growth promotion such as mineral phosphate solubilization and indole acetic acid (IAA) production [ 104 , 105 ] were found in several beneficial strains. IAA production from tryptophan can happen through different pathways whereas the ipd C gene from the indole-3-pyruvate (IPA) pathway and ami E and aam (homologous to iaa M) genes from the indole-3-acetamide (IAM) pathway were found in the strains ANP8 (IPA and IAM), C1 (IPA and IAM), and P5 (IAM) [ 106 , 107 , 108 ]. In addition, genes involved in the production of gluconic acid, an important organic acid responsible for mineral phosphate solubilization, were found in the beneficial strains.…”

“…In addition, genes involved in the production of gluconic acid, an important organic acid responsible for mineral phosphate solubilization, were found in the beneficial strains. Such genes include glucose dehydrogenase ( gcd ) and those related to pyrroloquinoline quinine synthesis, a coenzyme for gcd activity [ 109 ], reported in ANP8, C1, and P5 strains [ 106 , 107 , 108 ]. Interestingly, ANP8 harbors several genes involved in regulating potassium concentrations, which the authors associated with the salinity tolerance potential of this strain [ 106 ].…”

A Review of Recent Advances and Future Directions in the Management of Salinity Stress in Finger Millet

“…Salinity tolerance allows plants to endure elevated cellular sodium ion concentrations by preserving cell turgor and raising protoplasmic resistance ( Soliman et al, 2018 ). With an increasing number of genomics resources available for various plant lineages displaying different approaches and variation in salinity-stress avoidance or tolerance ( Bhardwaj et al, 2014 ; Razali et al, 2018 ; Chanwala et al, 2020 ; Noori et al, 2021 ), systems biology, a technique that utilizes genome-scale analyses of molecules and their interplay ( Shah et al, 2017 ), is emerging as an attractive approach to connect genes to salinity-stress avoidance or salinity-tolerance traits. Therefore, the utilization of synthetic biology technologies in developing finger millet plant lines impervious to salinity stress represents not only the accumulative insertions of transgenes but also the directed construction of entirely new metabolic pathways and networks, physiological traits, and growth and developmental control strategies.…”

Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops

“…Plant growth promotion mechanisms are related to the presence of several genes in the bacterial genomes whose analysis allows us to understand the adaptation of bacteria to plants [23,24], but few studies analyzing the genomes of PGPB endophytes inhabiting legume nodules have been carried out to date [12,17,18]. genome analysis can help to improve the selection process of PGPB and to commonly focus on the search for genes involved in metabolic pathways related to plant growth promotion [25,26].…”

“…These bacteria can inhabit the plant rhizosphere or the inner tissues of plants [6], plant endophytes being the most efficient inoculants [7]. For this reason, in the past year, several research studies have evaluated ways of increasing plant growth by using bacterial endophytes isolated from non-legume plants [8,9] and from legume nodules [10][11][12][13][14][15][16][17][18]. These nodules contain the rhizobia responsible for their formation and for symbiotic nitrogen fixation as well as other bacterial endophytes with different plant growth promotion mechanisms [19][20][21][22].…”