Whole-genome sequence of
Paenibacillus amylolyticus
strain W018, isolated from
Triticum aestivum
L. seeds, obtained using nanopore sequencing
Vladimir K. Chebotar,
Maria S. Gancheva,
Elena P. Chizhevskaya
et al.
Abstract:In this study, we performed nanopore sequencing of the genome of
Paenibacillus amylolyticus
strain W018, isolated from the seeds of winter wheat, cv. Bezostaya 100. The genome size is 7.07 Mb, with a GC content of 45.8%, and contains 8,190 genes.
“…Here, we report the genome of the K. cowanii strain that was isolated from seeds of winter wheat ( Triticum aestivum L.), cv. Bezostaya 100, as described previously ( 3 ). Genomic DNA was extracted from a single colony, grown on LB medium (Thermo Fisher Scientific, MA, USA), using the cetyltrimethylammonium bromide-NaCl method ( 4 ).…”
In this study, we sequence, assemble, and annotate
Kosakonia cowanii
strain W006, isolated from seeds of
Triticum aestivum
L. W006 has a single circular chromosome of 4,788,099 bp and 4,466 genes, with a mean G +C content of 56.1%.
“…Here, we report the genome of the K. cowanii strain that was isolated from seeds of winter wheat ( Triticum aestivum L.), cv. Bezostaya 100, as described previously ( 3 ). Genomic DNA was extracted from a single colony, grown on LB medium (Thermo Fisher Scientific, MA, USA), using the cetyltrimethylammonium bromide-NaCl method ( 4 ).…”
In this study, we sequence, assemble, and annotate
Kosakonia cowanii
strain W006, isolated from seeds of
Triticum aestivum
L. W006 has a single circular chromosome of 4,788,099 bp and 4,466 genes, with a mean G +C content of 56.1%.
“…Bacteria isolation was performed as previously described [10]. DNA was extracted from a single colony using the cetyltrimethylammonium bromide (CTAB)-NaCl method [11].…”
Section: Bacteria Isolation and Dna Extractionmentioning
We report the whole-genome sequences of the endophyte Curtobacterium flaccumfaciens strain W004 isolated from the seeds of winter wheat, cv. Bezostaya 100. The genome was obtained using Oxford Nanopore MinION sequencing. The bacterium has a circular chromosome consisting of 3.63 kbp with a G+C% content of 70.89%. We found that Curtobacterium flaccumfaciens strain W004 could promote the growth of spring wheat plants, resulting in an increase in grain yield of 54.3%. Sequencing the genome of this new strain can provide insights into its potential role in plant–microbe interactions.
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