Whole-Genome Sequence of Erwinia persicina B64, Which Causes Pink Soft Rot in Onions

Abstract

Erwinia persicina B64 was isolated from rotten onions in cold-storage facilities. Here, we report the complete genome sequence of E. persicina B64, which contains 5,070,450 bp with 55.17% GC content. The genome of this isolate is composed of one chromosome and two plasmids.

Genome analysis of Erwinia persicina reveals implications for soft rot pathogenicity in plants

“…This study has provided the first detailed insights in the genomic makeup of E. persicina as a soft rot agent, as well as genomic comparisons with a common soft rot agent, P. carotovorum , and other Erwinia pathogens that do not cause soft rot and non-pathogens. As of yet, there have been no publications investigating the genome of E. persicina other than to briefly describe the genome sequence of E. persicina strain B64 ( Cho et al, 2019 ). Our current work thus significantly expands the knowledge of the E. persicina genome by discussing the absence of the Out cluster of the T2SS, which is used by well-characterized soft rot pathogens to secrete pectolytic enzymes needed for disease, and the presence of a phenolic acid decarboxylase, a possible adaptation to survive within the plant host environment that is not present in other Erwinia plant pathogens.…”

“…Currently, E. persicina has a small known host range and characteristically causes pink soft rot on garlic, onion, lettuce, and parsley root ( Gálvez et al, 2015 ; Cho et al, 2019 ; Nechwatal and Theil, 2019 ; Canik Orel, 2020 ). Isolates SR13-16 caused rot symptoms on carrots, garlic cloves, and white and yellow onions ( Figure 3 ).…”

“…Genetic comparisons of the 16S rRNA genes of 29 Erwinia, Pantoea, and Enterobacter species found that the organisms that caused soft rot should be assigned their own genera, Pectobacterium and Dickeya ( Hauben et al, 1998 ; Samson et al, 2005 ). Recently, another species within the Erwinia genus, Erwinia persicina , has been identified as a soft rot agent ( Gálvez et al, 2015 ; Cho et al, 2019 ; Nechwatal and Theil, 2019 ; Canik Orel, 2020 ). Erwinia persicina causes pink-pigmented soft rot on a small range of plant hosts, including: garlic, onions, lettuce, mushrooms, barley and parsley root ( Gálvez et al, 2015 ; Cho et al, 2019 ; Nechwatal and Theil, 2019 ; Yan et al, 2019 ; Canik Orel, 2020 ; Kawaguchi et al, 2021 ).…”

“…Recently, another species within the Erwinia genus, Erwinia persicina , has been identified as a soft rot agent ( Gálvez et al, 2015 ; Cho et al, 2019 ; Nechwatal and Theil, 2019 ; Canik Orel, 2020 ). Erwinia persicina causes pink-pigmented soft rot on a small range of plant hosts, including: garlic, onions, lettuce, mushrooms, barley and parsley root ( Gálvez et al, 2015 ; Cho et al, 2019 ; Nechwatal and Theil, 2019 ; Yan et al, 2019 ; Canik Orel, 2020 ; Kawaguchi et al, 2021 ). Prior to the identification as a soft rot agent, E. persicina was classified as an epiphyte of cucumbers, tomatoes, and bananas; there has been one report of isolation from the urinary tract of a human ( Hao et al, 1990 ; O’Hara et al, 1998 ).…”

Genomic and morphological characteristics of the cold-adapted bacteria Mycetocola saprophilus provide insights into the pathogenesis of soft rot in Flammulina velutipes

“…To further understand the pathogenic mechanisms of soft rot disease, the complete genomes of some pathogenic bacteria strains have recently been sequenced and analyzed, including that of Erwinia persicina B64, which causes soft rot in onions [16], and that of Dickeya dianthicola ME23, which causes soft rot in potatoes [17]. However, the pathogenic mechanisms of soft rot disease in F. velutipes have not been elucidated.…”