Whole-genome sequence analysis of Vibrio cholerae from three outbreaks in Uganda, 2014 - 2016

Aruhomukama, Dickson; Sserwadda, Ivan; Mboowa, Gerald

doi:10.12688/f1000research.20048.1

Cited by 4 publications

(3 citation statements)

References 23 publications

(4 reference statements)

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“…Additionally, the isolate was resistant to pulvomycin, which was horizontally transmitted to V. cholerae. A similar antibiotic resistance mechanism was previously reported in the Uganda study during 2014-2016 [53].…”

Section: Discussionsupporting

confidence: 84%

Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant Vibrio cholerae VC01 Isolated from a Clinical Sample

et al. 2023

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Cholera, a disease caused by the Vibrio cholerae bacteria, threatens public health worldwide. The organism mentioned above has a significant historical record of being identified as a prominent aquatic environmental pollutant capable of adapting its phenotypic and genotypic traits to react to host patients effectively. This study aims to elucidate the heterogeneity of the sporadic clinical strain of V. cholerae VC01 among patients residing in Silvasa. The study involved conducting whole-genome sequencing of the isolate obtained from patients exhibiting symptoms, including those not commonly observed in clinical practice. The strain was initially identified through a combination of biochemical analysis, microscopy, and 16s rRNA-based identification, followed by type strain-based identification. The investigation demonstrated the existence of various genetic alterations and resistance profiles against multiple drugs, particularly chloramphenicol (catB9), florfenicol (floR), oxytetracycline (tet(34)), sulfonamide (sul2), and Trimethoprim (dfrA1). The pan-genomic analysis indicated that 1099 distinct clusters were detected within the genome sequences of recent isolates worldwide. The present study helps to establish a correlation between the mutation and the coexistence of antimicrobial resistance toward current treatment.

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Section: Discussionsupporting

confidence: 84%

Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant Vibrio cholerae VC01 Isolated from a Clinical Sample

et al. 2023

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“…The MakA (motility associated killing factor A) is a agella-mediated toxin secreted via the fT3SS. It was rst described in 2018 as pathogenic in Caenorhabditis elegans and zebra sh later reported from Tanzania in reservoirs of Vibrio cholera O1 strains and from epidemics occurring 2015-2017 and also found in sequenced strains from Uganda O1 cholera outbreaks 2014-2016 (55)(56)(57)(58). We conclude that the combination of virulence factors of our isolate suggests the nding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version.…”

Section: Resultssupporting

confidence: 49%

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

Westerström

Dragsted

2022

Preprint

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Purpose (stating the main purposes and research question) Vibrio cholerae is classified in O-antigen polysaccharide outer membrane properties where O1 and O139 are strains that cause pandemics and epidemics while non-O1/non-O139 usually cause mild disease. The dynamic evolution of Vibrio cholerae involves new virulence factors through horizontal gene transfer and formerly nontoxigenic serogroups are being reported as causing increasingly severe forms of human disease. Our purpose was to investigate serogroup and virulence factors in one imported isolate from Vietnam and compare these to virulence factors seen in different strains of Vibrio cholerae. Methods This is an observational study where we have serotyped one isolate of imported Vibrio cholerae from Vietnam to Denmark and performed whole genome sequencing to identify known virulence genes and furthermore studied the epidemiology of virulence in pathogenic strains of Vibrio cholerae. Results We have identified virulence factors in our isolate that are recently discovered and one toxin, MakA, has not previously been reported from an Asian strain. The isolate was found to be a serogroup non-O1/non-O139 strain, however, sequence analysis gave a 96, 6% ID match of the wbfZ gene, stipulating our isolate to belong to either serogroup O22 or O139. Conclusions Looking at the combination of virulence factors of the isolate we suggest the finding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version. This is a rare finding in a clinical isolate.

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“…The MakA (motility associated killing factor A) is a agella-mediated toxin secreted via the fT3SS. It was rst described in 2018 as pathogenic in Caenorhabditis elegans and zebra sh later reported from Tanzania in reservoirs of Vibrio cholera O1 strains and from epidemics occurring 2015 -2017 and also found in sequenced strains from Uganda O1 cholera outbreaks 2014 -2016 (55)(56)(57)(58). We conclude that the combination of virulence factors of our isolate suggests the nding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version (Figure 2).…”

Section: Discussionsupporting

confidence: 52%

A Rough Variant of Nontoxigenic Vibrio Cholerae O139 Imported From Vietnam

Westerström

Dragsted

2021

Preprint

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Purpose (stating the main purposes and research question)Vibrio cholerae is classified in O-antigen polysaccharide outer membrane properties where O1 and O139 are strains that cause pandemics and epidemics while non-O1/non-O139 usually cause mild disease. The dynamic evolution of Vibrio cholerae involves new virulence factors through horizontal gene transfer and formerly nontoxigenic serogroups are being reported as causing increasingly severe forms of human disease. Our purpose was to investigate serogroup and virulence factors in one imported isolate from Vietnam and compare these to virulence factors seen in different strains of Vibrio cholerae.MethodsWe have serotyped one isolate of imported Vibrio cholerae from Vietnam to Denmark and performed whole genome sequencing to identify known virulence genes. ResultsWe have identified virulence factors in our isolate that are recently discovered and one toxin, MakA, has not previously been reported from an Asian strain. The isolate was found to be a serogroup non-O1/non-O139 strain, however, sequence analysis gave a 96, 6% ID match of the wbfZ gene, stipulating our isolate to belong to either serogroup O22 or O139. ConclusionsLooking at the combination of virulence factors of the isolate we suggest the finding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version. This is a rare finding in a clinical isolate.

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Whole-genome sequence analysis of Vibrio cholerae from three outbreaks in Uganda, 2014 - 2016

Cited by 4 publications

References 23 publications

Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant Vibrio cholerae VC01 Isolated from a Clinical Sample

Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant Vibrio cholerae VC01 Isolated from a Clinical Sample

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

A Rough Variant of Nontoxigenic Vibrio Cholerae O139 Imported From Vietnam

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