Whole genome sequence analysis of bacteriophage P1 that infects the Lactobacillus plantarum

Inactivation of Lactobacillus Bacteriophages by Dual Chemical Treatments

“…In our previous research, the morphology of phages P1 and P2 were similar, but their structures were slightly different. The tail fibrin (CDs18) of phage P1 was different from that encoded in phage P2’s genome ( Guo et al 2022 ). The two electrostatic environments caused by different tail fibrin may enable isopropanol to form isopropanol-water clusters with different stability in aqueous solution, thus affecting the biological efficacy of isopropanol on phages P1 and P2.…”

“…Our previous studies have shown 11 gene differences between these two phages, including eight putative proteins, one tail fibrin, and two HNH endonucleases. Moreover, phage P2 encoded more putative proteins with unknown functional gene sequences ( Guo et al 2022 ; Zhu et al 2022 ). The difference in protein structure and the gene sequence coding proteins may be why phages P1 and P2 have different tolerance to sodium hypochlorite.…”

“…Its whole genome was composed of linear dsDNA, with 73,787 bp in length and 86 predicted CDs. The GC content was 39.17% ( Guo et al 2022 ). When compared to phage P1, phage P2 was smaller, with an isometric capsid about 66.7 ± 3.0 nm and a long noncontractile tail (216.7 ± 3.0 nm long, 12.3 ± 3.0 nm wide) ( Chen et al 2019 ).…”