Whole genome sequence analysis of Mycobacterium suricattae

Dippenaar, Anzaan; Parsons, Sven D.C.; Sampson, Samantha L.; Merwe, Ruben Gerhard van der; Drewe, Julian Ashley; Abdallah, Abdallah; Siame, Kabengele Keith; Pittius, Nicolaas C. Gey van; Helden, Paul D. van; Pain, Arnab; Warren, Robin M.

doi:10.1016/j.tube.2015.10.001

Cited by 40 publications

(40 citation statements)

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“…These deletions, known as region of difference (RD)5, have occurred independently in M. bovis / M. caprae , M. orygis , M. suricattae and M. mungi , the Dassie Bacillus and a subset of M. microti strains isolated from voles (Brodin et al , ; Garnier et al , ; Mostowy et al , ; Dippenaar et al , ; Brites et al , ). In fact, the only known animal adapted MTBC members that have retained the RD5 locus are the Chimpanzee bacillus, M. pinnipedii and a subset of M. microti isolates isolated from humans (Brodin et al , ; Coscolla et al , ; Brites et al , ).…”

Section: Implications Of Pe and Ppe Gene Variation For The Evolution mentioning

confidence: 99%

New insights into the mycobacterial PE and PPE proteins provide a framework for future research

Ates

2019

Molecular Microbiology

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The PE and PPE proteins of Mycobacterium tuberculosis have been studied with great interest since their discovery. Named after the conserved proline (P) and glutamic acid (E) residues in their N-terminal domains, these proteins are postulated to perform wide-ranging roles in virulence and immune modulation. However, technical challenges in studying these proteins and their encoding genes have hampered the elucidation of molecular mechanisms and leave many open questions regarding the biological functions mediated by these proteins. Here, I review the shared and unique characteristics of PE and PPE proteins from a molecular perspective linking this information to their functions in mycobacterial virulence. I discuss how the different subgroups (PE_PGRS, PPE-PPW, PPE-SVP and PPE-MPTR) are defined and why this classification of paramount importance to understand the PE and PPE proteins as individuals and or groups. The goal of this MicroReview is to summarize and structure the existing information on this gene family into a simplified framework of thinking about PE and PPE proteins and genes. Thereby, I hope to provide helpful starting points in studying these genes and proteins for researchers with different backgrounds. This has particular implications for the design and monitoring of novel vaccine candidates and in understanding the evolution of the M. tuberculosis complex.

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Section: Implications Of Pe and Ppe Gene Variation For The Evolution mentioning

confidence: 99%

New insights into the mycobacterial PE and PPE proteins provide a framework for future research

Ates

2019

Molecular Microbiology

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“…Obligatory pathogens include species belonging to the Mycobacterium tuberculosis complex ( M. tuberculosis, M. bovis, M. africanum, M. caprae, M. microti, M. pinnipedii, M. leprae, M. canetti and recently described M. orygis, M. mungi and M. suricattae 4 - 6 ). The potentially pathogenic species are represented by the M. avium intracellulare complex ( M. avium subspecies avium, M. avium paratuberculosis, M. avium hominissuis, M. avium silvaticum, M. colombiense and M. indicus pranii ) , M. marinum, M. fortuitum, M. scrofulaceum, M. kansasii, M. xenopi , among others.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of mycobacteria in bovine milk: an overview

Bolaños

Paula

Guerra

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Tuberculosis remains as the world’s biggest threat. In 2014, human tuberculosis ranked as a major infectious disease by the first time, overcoming HIV death rates. Bovine tuberculosis is a chronic disease of global distribution that affects animals and can be transmitted to humans by the consumption of raw milk, representing a serious public health concern. Despite the efforts of different countries to control and eradicate bovine tuberculosis, the high negative economic impact on meat and milk production chains remains, given the decreased production efficiency (approximately 25%), the high number of condemned carcasses, and increased animal culling rates. This scenario has motivated the establishment of official programs based on regulations and diagnostic procedures. Although Mycobacterium tuberculosis and Mycobacterium bovis are the major pathogenic species to humans and bovines, respectively, nontuberculous mycobacteria within the Mycobacterium genus have become increasingly important in recent decades due to human infections, including the ones that occur in immunocompetent people. Diagnosis of mycobacteria can be performed by microbiological culture from tissue samples (lymph nodes, lungs) and secretions (sputum, milk). In general, these pathogens demand special nutrient requirements for isolation/growth, and the use of selective and rich culture media. Indeed, within these genera, mycobacteria are classified as either fast- or slow-growth microorganisms. Regarding the latter ones, incubation times can vary from 45 to 90 days. Although microbiological culture is still considered the gold standard method for diagnosis, molecular approaches have been increasingly used. We describe here an overview of the diagnosis of Mycobacterium species in bovine milk.

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“…However, it is currently unclear which components of the PC-HP antigens might be responsible for this immune sensitization. The genetic region of difference 1 (RD1), a variant of which is deleted from the M. suricattae genome [15], encodes both ESAT-6 and CFP-10 and is also required for the secretion of Esx-1 substrate protein C (espC) which is encoded by the gene Rv3615c [16]. While Rv 3615 c is present in the M. suricattae genome (pers.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a diagnostic cytokine-release assay for Mycobacterium suricattae infection in meerkats (Suricata suricatta)

et al. 2016

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BackgroundSensitive diagnostic tools are necessary for the detection of Mycobacterium suricattae infection in meerkats (Suricata suricatta) in order to more clearly understand the epidemiology of tuberculosis and the ecological consequences of the disease in this species. We therefore aimed to develop a cytokine release assay to measure antigen-specific cell-mediated immune responses of meerkats.ResultsEnzyme-linked immunosorbent assays (ELISAs) were evaluated for the detection of interferon-gamma (IFN-γ) and IFN-γ inducible protein 10 (IP-10) in meerkat plasma. An IP-10 ELISA was selected to measure the release of this cytokine in whole blood in response to Bovigam® PC-HP Stimulating Antigen, a commercial peptide pool of M. bovis antigens. Using this protocol, captive meerkats with no known M. suricattae exposure (n = 10) were tested and results were used to define a diagnostic cut off value (mean plus 2 standard deviations). This IP-10 release assay (IPRA) was then evaluated in free-living meerkats with known M. suricattae exposure, categorized as having either a low, moderate or high risk of infection with this pathogen. In each category, respectively, 24.7%, 27.3% and 82.4% of animals tested IPRA-positive. The odds of an animal testing positive was 14.0 times greater for animals with a high risk of M. suricattae infection compared to animals with a low risk.ConclusionThese results support the use of this assay as a measure of M. suricattae exposure in meerkat populations. Ongoing longitudinal studies aim to evaluate the value of the IPRA as a diagnostic test of M. suricattae infection in individual animals.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0927-x) contains supplementary material, which is available to authorized users.

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Whole genome sequence analysis of Mycobacterium suricattae

Cited by 40 publications

References 37 publications

New insights into the mycobacterial PE and PPE proteins provide a framework for future research

New insights into the mycobacterial PE and PPE proteins provide a framework for future research

Diagnosis of mycobacteria in bovine milk: an overview

Development and evaluation of a diagnostic cytokine-release assay for Mycobacterium suricattae infection in meerkats (Suricata suricatta)

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