Whole genome re-sequencing of two ‘wild-type’ strains of the model cyanobacteriumSynechocystissp. PCC 6803

Abstract

The photoautotrophic cyanobacterium Synechocystis sp. PCC 6803 is a widely used model in genomic research and was the first photosynthetic organism to have its entire genome sequenced. Here, we report the genome sequence for two glucose-tolerant laboratory 'wild-type' strains, GT-O1 and GT-O2, in use at the University of Otago. Using high-throughput genome sequencing techniques and subsequent Sanger sequencing of detected variants, we have identified ten de novo mutations in the two strains, of which six are unique to GT-O2 cells. The presence of these unique mutations highlights the need to know the genomic background of the parent strain when constructing mutants in Synechocystis sp. PCC 6803 strains.

Association of Psb28 and Psb27 Proteins with PSII-PSI Supercomplexes upon Exposure of Synechocystis sp. PCC 6803 to High Light

“…Since this disagreement could be caused by differences in the genetic backgrounds of the two WT strains used to make the mutants, we constructed two new Psb28-1-and Psb28-2-less mutants using a different WT strain. In our original study (Dobá ková et al, 2009), we used a WT strain very similar to the glucose-tolerant GT-O2 strain (Morris et al, 2014), which was subsequently shown to exhibit partial Chl depletion upon autotrophic growth conditions (GT-W; Tichý et al, 2016). The new set of mutants was instead constructed in the GT-P strain similar to the GT-Kazusa strain (Supplemental Figure 6), which contains a similar content of Chl as the standard motile PCC 6803 strain (Tichý et al, 2016).…”

The D1:Ser268 residue of Photosystem II contributes to an alternative pathway for QB protonation in the absence of bound bicarbonate

“…The cyanobacterium used for this study was the GT-O1 glucose-tolerant strain of Synechocystis sp. PCC 6803 (hereafter referred to as Synechocystis 6803) [16,17]. The control and S268A and S268T strains were generated as described in Forsman et al [18].…”

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803

“…The direction of movement correlates with localization of PilB patches at the inner membrane . The identification of key genes involved in the motility of Synechocystis began from the observation of some spontaneous mutants with different motility (Tajima et al, 2011;Kanesaki et al, 2012;Trautmann et al, 2012;Morris et al, 2014;Ding et al, 2015). There are two distinct major groups of Synechocystis substrains: the motile PCC and non-motile GT-lineages (Morris et al, 2017).…”