Whole genome microarray analysis in non-small cell lung cancer

Zeyadi, Mohammad Al; Dimova, Ivanka; Ranchich, Vladislav; Rukova, Blaga; Nesheva, Desislava; Hamude, Zora; Georgiev, S.; Petrov, Danail; Toncheva, Draga

doi:10.1080/13102818.2014.989179

Cited by 17 publications

(10 citation statements)

References 15 publications

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“…In most cases, SOX5 acts as an oncogene in various tumors such as NSCLC, hepatocellular carcinoma, and glioma. 25 , 46 , 47 An earlier report has shown that SOX5 is a frequent tumor antigen in glioma and is related to diagnosis and prognosis in glioma patients. 25 Our results showed that SOX5 was upregulated in glioma tissues and cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of TDP43-Mediated SNHG12-miR-195-SOX5 Feedback Loop Impeded Malignant Biological Behaviors of Glioma Cells

Liu

Zheng

Xue

et al. 2018

Molecular Therapy - Nucleic Acids

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Long non-coding RNA (lncRNA) dysregulation is involved in tumorigenesis and regulation of diverse cellular processes in gliomas. lncRNA SNHG12 is upregulated and promotes cell growth in human osteosarcoma cells. TAR-DNA binding protein 43 (TDP43) functions as an oncogene in various tumors by modulating RNA expression. Downregulation of TDP43 or SNHG12 significantly inhibited malignant biological behaviors of glioma cells. miR-195, downregulated in glioma tissues and cells, significantly impaired the malignant progression of glioma cells. TDP43 upregulated miR-195 in an SNHG12-dependent manner. We further revealed that SNHG12 and miR-195 were in an RNA-induced silencing complex (RISC). Inhibition of SNHG12 combined with restoration of miR-195 robustly reduced tumor growth in vivo. SOX5 was overexpressed in glioma tissues and cells. miR-195 targeted SOX5 3′ UTR in a sequence-specific manner. Gelsolin was activated by SOX5. More importantly, SOX5 activated SNHG12 promoter and upregulated its expression, forming a feedback loop. Dysregulation of SNHG12, miR-195, and SOX5 predicted poor prognosis of glioma patients. The present study demonstrated that SNHG12-miR-195-SOX5 feedback loop exerted a crucial role in the regulation of glioma cells’ malignant progression.

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Section: Discussionmentioning

confidence: 99%

Inhibition of TDP43-Mediated SNHG12-miR-195-SOX5 Feedback Loop Impeded Malignant Biological Behaviors of Glioma Cells

Liu

Zheng

Xue

et al. 2018

Molecular Therapy - Nucleic Acids

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“…However, in this study, miR-582-5p was greatly up-regulated in the blood of patients with lung cancer. ETV1 is a DE TF regulated by miR-582-5p, which was recently proposed as an oncogene in non-small cell lung cancer [ 23 ]. Furthermore, WT1 as a Wilms’ tumor gene was found to be overexpressed in various types of cancers [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Crosstalk in competing endogenous RNA network reveals the complex molecular mechanism underlying lung cancer

et al. 2017

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We investigated the transcriptional mechanism underlying lung cancer development. RNA sequencing analysis was performed on blood samples from lung cancer cases and healthy controls. Differentially expressed microRNAs (miRNAs), circular RNAs (circRNAs), mRNAs (genes), and long non-coding RNAs (lncRNA) were identified, followed by pathway enrichment analysis. Based on miRNA target interactions, a competing endogenous network was established and significant nodes were screened. Differentially expressed transcriptional factors were retrieved from the TRRUST database and the transcriptional factor regulatory network was constructed. The expression of 59 miRNAs, 18,306 genes,232 lncRNAs, and 292 circRNAs were greatly altered in patients with lung cancer. miRNAs were closely associated with cancer-related pathways, such as pathways in cancer, colorectal cancer, and transcriptional misregulation in cancer. Two novel pathways, olfactory transduction and neuroactive ligand-receptor interactions, were significantly enriched by differentially expressed genes. The competing endogenous RNA network revealed 5 hub miRNAs. Hsa-miR-582-3p and hsa-miR-582-5p were greatly enriched in the Wnt signaling pathway. Hsa-miR-665 was closely related with the MAPK signaling pathway. Hsa-miR-582-3p and hsa-miR-582-5p were also present in the TF regulatory network. Transcriptional factors of WT1 (wilms tumor 1) and ETV1 (ETS variant 1) were regulated by hsa-miR-657 and hsa-miR-582-5p, respectively, and controlled androgen receptor gene expression. miR-582-5p, miRNA-582-3p, and miR-657 may play critical regulatory roles in lung tumor development. Our work may explore new mechanism of lung cancer and aid the development of novel therapy.

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“…1c). Despite having not been previously associated with MM, region 8p23.1 contains the beta-defensin gene cluster (DEFB) ( Supplementary Table S1) which has been shown to be highly copy number variable and associated with prostate cancer and non-small cell lung cancer [27][28][29]. Additionally, region 2q31.1 containing the HOXD gene cluster was also significantly lost (q = 0.097; Fig.…”

Section: Pleural Effusions Allow Detection Of Novel Regions Of Interestmentioning

confidence: 97%

Malignant cells from pleural fluids in malignant mesothelioma patients reveal novel mutations

et al. 2018

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Whole genome microarray analysis in non-small cell lung cancer

Cited by 17 publications

References 15 publications

Inhibition of TDP43-Mediated SNHG12-miR-195-SOX5 Feedback Loop Impeded Malignant Biological Behaviors of Glioma Cells

Inhibition of TDP43-Mediated SNHG12-miR-195-SOX5 Feedback Loop Impeded Malignant Biological Behaviors of Glioma Cells

Crosstalk in competing endogenous RNA network reveals the complex molecular mechanism underlying lung cancer

Malignant cells from pleural fluids in malignant mesothelioma patients reveal novel mutations

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