Whole‐genome copy number and immunohistochemical analyses on surgically resected intracholecystic papillary neoplasms

Abstract

Intracholecystic papillary neoplasms are newly defined precancerous lesions. According to Classification of the World Health Organization, they have four histological morphologies, which are biliary, gastric, intestinal, and oncocytic. This study evaluated 17 patients with resected intracholecystic papillary neoplasms in terms of histological, immunohistochemical, and copy number variation (CNV). The histological subtypes included 5 cases of low‐grade (5 gastric) and 12 cases of high‐grade (6 gastric and 6 biliary) neoplasms. Most cases showed high expression of MUC1, MUC5AC, and CK7, moderate expression of MUC6 and Ki‐67, and low expression of CK20, MUC2, and CDX2. The CNV profile identified gain of 7q in 12%, and loss of 1p (18%), 5q (29%), 9p (35%), 12p (17%), 17p (24%), and 19p (18%). No CNVs were observed in low‐grade neoplasms, whereas high‐grade ones had increasing abnormalities. β‐catenin was often expressed in the nucleus of neoplasms with gastric morphology, suggesting the involvement of the Wnt/β‐catenin pathway. However, it was not expressed among those with biliary morphology, which instead exhibited high p53 expression. Neoplasms with biliary morphology showed more CNV changes (9p, 17p, 19p losses). Distinct immunological and CNV patterns were seen in both morphologies, suggesting differences in their pathogenesis. More CNVs accumulated with tumor progression.

Natural history of intracholecystic papillary neoplasm (ICPN): a rare case of ICPN whose natural history was closely followed by ultrasound

“…The accumulation of genetic mutations with tumor progression was suggested in the previous case series of ICPN, and ICPN exhibited slight p53 and MIB-1 staining, reflecting its mutation in a previous case series [ 18 ]. Positive staining for β-catenin suggested activation of the Wnt/β-catenin pathway in ICPN [ 19 ].…”

Artificial Neural Networks Predicted the Overall Survival and Molecular Subtypes of Diffuse Large B-Cell Lymphoma Using a Pancancer Immune-Oncology Panel

“…Additionally, the risk-score formula integrated all genes, and two groups with different risk could be found among the results. Macrophages release interferon alpha-8 ( IFNA8 ) [ 26 ], and we found that the high-risk group was associated with high expression of CD163 , which is a marker of M2-like tumor-associated macrophages (TAMs). Caspase-1 ( CASP1 ) is involved in various inflammatory processes and initiates programmed cell death [ 31 ], and we found that the high-risk group also associated with high MYD88 expression.…”

“…The staining was performed using a Leica Bon-Max slide stainer (Leica Biosystems K.K. Tokyo, Japan) following the manufacturer’s instructions [ 26 ], Bond epitope retrieval solution 1 (pH 6.0, 30 min., #AR9961, Leica), and at a 1:400 dilution. After staining, the slides were scanned in a NanoZoomer S360 digital slide scanner (#C13220-01, Hamamatsu K.K.…”

Artificial Intelligence Analysis of Gene Expression Predicted the Overall Survival of Mantle Cell Lymphoma and a Large Pan-Cancer Series

“…The immunohistochemistry was performed using an automated piece of equipment, Leica BOND-MAX stainer, following the manufacturer’s instructions and as previously described [ 53 , 59 , 63 , 64 , 65 ]. The RGS1 primary antibody (rabbit polyclonal) was purchased from Thermofisher [ 63 ].…”