Whole Genome Characterization and Genetic Evolution Analysis of a New Ostrich Parvovirus

Yuan, Kehong; Wang, Dongdong; Luan, Qingdong; Sun, Ju; QianWen, Gao; Jiang, Zhiyao; Wang, Shouchun; Han, Yijun; Qu, Xueting; Cui, Yueying; Qiu, Shimei; Di, Youxia; Wang, Xiaoyi; Song, Shaofu; Wang, Peiheng; Xia, Shilong; Yu, Yongle; Liu, Weiquan; Yin, Ye

doi:10.3390/v12030334

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…In this study, we investigated the pathogen that caused this disease in ostrich farms and detected OsPV in the samples collected from the diseased ostriches. Tests for other viral agents, such as AIV, APMV-1, AEV, ARV, and MS were negative, which is consistent with the findings of a previous study ( Yuan et al, 2020 ). The vaccine generated by inactivating GPV can prevent and treat ostriches to a certain extent, therefore, we speculate that ostrich parvovirus (OsPV) was the pathogen causing ostrich diarrhea and paralysis; however, this needs further investigation.…”

Section: Discussionsupporting

confidence: 91%

“…Interestingly, the genome of OsPV possesses 2 deletions at the ITR compared to the GPV vaccine SYG61v and the virulent B strains ( Figure 4 B). The ITR not only functions as an origin of genome replication but also contains several transcription factor binding sites, including E-box, activating transcription factor/cyclic AMP-responsive element binding (ATF/CREB), and an upstream stimulatory factor known as MLTF ( Yuan et al, 2020 ). The deletion sequence in the ITR may be one of the reasons why the GPV infects ostriches.…”

Section: Discussionmentioning

confidence: 99%

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Emergence and genomic analysis of a novel ostrich-origin GPV-related parvovirus in China

et al. 2022

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Emergence and genomic analysis of a novel ostrich-origin GPV-related parvovirus in China

et al. 2022

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“…4, Table 3 ) [18]. Interestingly, recent studies have reported the spread of autonomous parvoviruses related to MDPV and GPV from anseriform birds to the relatively distantly related ratites (flightless birds) [55], suggesting that these viruses might have an inherent capacity to cross between relatively distantly related host groups, and may represent emerging pathogens of poultry and wild birds.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis reveals the long-term co-evolutionary history of parvoviruses and vertebrates

Campbell

Loncar

Kotin

et al. 2021

Preprint

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Parvoviruses (family Parvoviridae) are small, non-enveloped DNA viruses that infect a broad range of animal species. Comparative studies, supported by experimental evidence, show that many vertebrate species contain sequences derived from ancient parvoviruses embedded in their genomes. These endogenous parvoviral elements (EPVs), which arose via recombination-based mechanisms in infected germline cells of ancestral organisms, constitute a form of molecular fossil record that can be used to investigate the origin and evolution of the parvovirus family. Here, we use comparative approaches to investigate 198 EPV loci, represented by 470 EPV sequences identified in a comprehensive in silico screen of 752 published vertebrate genomes. We investigated EPV loci by constructing an open resource that contains the data items required for comparative sequence analysis of parvoviruses and uses a relational database to represent the complex semantic relationships between them. We used this standardised framework to implement reproducible comparative phylogenetic analysis of combined EPV and virus data. Our analysis reveals that viruses closely related to contemporary parvoviruses have circulated among vertebrates since the Late Cretaceous epoch (100-66 million years ago). We present evidence that the subfamily Parvovirinae, which includes ten vertebrate-specific genera, has evolved in broad congruence with the emergence and diversification of major vertebrate groups. Furthermore, we infer defining aspects of evolution within individual parvovirus genera - mammalian vicariance for protoparvoviruses (genus Protoparvovirus), and inter-class transmission for dependoparvoviruses (genus Dependoparvovirus) - thereby establishing an ecological and evolutionary perspective through which to approach analysis of these virus groups. We also identify evidence of EPV expression at RNA level and show that EPV coding sequences have frequently been maintained during evolution, adding to a growing body of evidence that EPV loci have been co-opted or exapted by vertebrate species, and especially by mammals. Our findings offer fundamental insights into parvovirus evolution. In addition, we establish novel genomic resources that can advance the development of parvovirus-related research - including both therapeutics and disease prevention efforts - by enabling more efficient dissemination and utilisation of relevant, evolution-related domain knowledge.

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Isolation of a feline-derived feline panleukopenia virus with an A300P substitution in the VP2 protein and confirmation of its pathogenicity in dogs

Li,

Peng,

Zeng

et al. 2024

Animal Diseases

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Feline panleukopenia virus (FPV) is a single-stranded DNA virus that can infect cats and cause feline panleukopenia, which is a highly contagious and fatal disease in felines. The sequence of FPV is highly variable, and mutations in the amino acids of its capsid protein play crucial roles in altering viral virulence, immunogenicity, host selection, and other abilities. In this study, the epidemiology of FPV was studied using 746 gastrointestinal swab samples derived from cats that presented gastrointestinal symptoms specifically, diarrhea or vomiting during the period spanning from 2018 to 2022. The overall prevalence of FPV-positive patients among these samples was determined to be 45.4%. Capsid (virion) protein 2 (VP2) gene of each FPV-positive sample was sequenced and amplified, yielding 65 VP2 sequences. Among them, six VP2 gene sequences were detected in the majority of the samples test positive for FPV, and these positive samples originated from a diverse range of geographical locations. These isolates were named FPV-6, FPV-10, FPV-15, FPV-251, FPV-271 and FPV-S2. Additionally, the substitution of Ala300Pro (A300P) in VP2 was detected for the first time in feline-derived FPV (FPV-251). FPV-251 isolate, with this substitution in VP2 protein, exhibited stable proliferative capacity in Madin-Darby canine kidney (MDCK) cells and A72 cells. FPV-271 was selected as the FPV control isolate due to its single amino acid difference from VP2 protein of FPV-251 at position 300 (FPV-271 has alanine, while FPV-251 has proline). After oral infection, both FPV-251 and FPV-271 isolates caused feline panleukopenia, which is characterized by clinical signs of enterocolitis. However, FPV-251 can infect dogs through the oral route and cause gastrointestinal (GI) symptoms with lesions in the intestine and mesenteric lymph nodes (MLNs) of infected dogs. This is the first report on the presence of an A300P substitution in VP2 protein of feline-derived FPV. Additionally, FPV isolate with a substitution of A300P at VP2 protein demonstrated efficient replication capabilities in canine cell lines and the ability to infect dogs.

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Whole Genome Characterization and Genetic Evolution Analysis of a New Ostrich Parvovirus

Cited by 3 publications

References 19 publications

Emergence and genomic analysis of a novel ostrich-origin GPV-related parvovirus in China

Emergence and genomic analysis of a novel ostrich-origin GPV-related parvovirus in China

Comparative analysis reveals the long-term co-evolutionary history of parvoviruses and vertebrates

Isolation of a feline-derived feline panleukopenia virus with an A300P substitution in the VP2 protein and confirmation of its pathogenicity in dogs

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