Whole genome analysis provides evidence for porcine-to-simian interspecies transmission of rotavirus-A

Navarro, Ryan; Aung, Meiji Soe; Cruz, Katalina; Ketzis, Jennifer; Gallagher, Christa A.; Beierschmitt, Amy; Malik, Yashpal Singh; Kobayashi, Nobumichi; Ghosh, Souvik

doi:10.1016/j.meegid.2016.12.026

Cited by 18 publications

(17 citation statements)

References 25 publications

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“…Detection of human-like VP6 gene in PoRVCs is in agreement with the report of Gabbay et al (2008). It is possible that animals might serve as reservoir host for re-assortment of RVs from different host origin (Mascarenhas et al 2007;Gabbay et al 2008;Navarro et al 2017). RVC infection in pigs becomes more important as the main livelihood framework of rural and tribal population that dominates the northeastern states of India is piggery.…”

Section: Discussionsupporting

confidence: 82%

“…Hitherto reports mention co-infection of different RV genotypes in pigs (Nyaga et al 2015). Hence, a chance for re-assortment in swine cannot be overlooked until the extensive screening of pig population for the presence of re-assorted or new RV types across the globe (Steyer et al 2007;Medici et al 2011;Sharma et al 2013;Navarro et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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Background: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. Objective: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. Methods: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Results: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. Conclusion:The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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“…During a study on porcine rotavirus‐A (RVA), 63 diarrhoeic faecal samples (50 piglets and 13 adult pigs) were collected from 21 pig farms across St. Kitts in August 2015 (Navarro et al., 2017) (Figure 1). Four of the porcine faecal samples tested positive for RVA (Navarro et al., 2017). On the other hand, kidneys showing gross lesions (foci of discoloration scattered on the cortical surface) were obtained from 61 slaughter age, clinically healthy pigs (as certified by the veterinarian during ante‐mortem examination) at the St. Kitts abattoir between July 2012 and March 2013 (Ghosh et al., 2018) (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…Phylogenetic analysis using the neighbor-joining method is shown in Supplementary Material S3 was based on analysis of archival porcine faecal and kidney samples from St. Kitts. During a study on porcine rotavirus-A (RVA), 63 diarrhoeic faecal samples (50 piglets and 13 adult pigs) were collected from 21 pig farms across St. Kitts in August 2015(Navarro et al, 2017) (Figure 1). Four of the porcine faecal samples tested positive for RVA(Navarro et al, 2017).…”

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confidence: 99%

High rates of detection and complete genomic analysis of porcine circovirus 2 (PCV2) in the Lesser Antilles island of St. Kitts: Identification of PCV2b‐PCV2d recombinants

Kleymann

Soto

Illanes

et al. 2020

Transbound. Emerg. Dis.

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Porcine circovirus 2 (PCV2), a member of the family Circoviridae, is an economically important pathogen of swine (Segalés, Kekarainen, & Cortey, 2013). PCV2 has been associated with a variety of porcine disease conditions that include post-weaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS), porcine respiratory disease complex (PRDC), reproductive disorders and enteritis (Opriessnig, Meng, & Halbur, 2007; Segalés, 2012). These clinical and subclinical manifestations, collectively known as porcine circovirus-associated diseases (PCVAD), cause significant economic losses to the pork industry worldwide (Opriessnig et al., 2007).

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“…There is evidence for a common origin of human Wa-like and porcine strains as well as human DS-1-like and bovine strains [9]. Zoonotic transmission of rotavirus strains and genome reassortment events between animal and human strains have been reported [10][11][12][13][14][15][16][17]. Mixed infections of animal rotavirus strains in animals, as well as mixed infections of human strains in human samples, have also been reported [18][19][20].…”

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confidence: 99%

First isolation and whole-genome characterization of a G9P[14] rotavirus strain from a diarrheic child in Egypt

Shoeib

Portocarrero

Wang

et al. 2020

Journal of General Virology

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An unusual group A rotavirus (RVA) strain (RVA/Human-tc/EGY/AS997/2012/G9[14]) was isolated for the first time in a faecal sample from a 6-month-old child who was hospitalized for treatment of acute gastroenteritis in Egypt in 2012. Whole-genome analysis showed that the strain AS997 had a unique genotype constellation: G9-P[14]-I2-R2-C2-M2-A11-N2-T1-E2-H1. Phylogenetic analysis indicated that the strain AS997 had the consensus P[14] genotype constellation with the G9, T1 and H1 reassortment. This suggests either a mixed gene configuration originated from a human Wa-like strain and a P[14]-containing animal virus, or that this P[14] could have been acquired via reassortment of human strains only. The study shows the possible roles of interspecies transmission and multiple reassortment events leading to the generation of novel rotavirus genotypes and underlines the importance of whole-genome characterization of rotavirus strains in surveillance studies.

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Whole genome analysis provides evidence for porcine-to-simian interspecies transmission of rotavirus-A

Cited by 18 publications

References 25 publications

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

High rates of detection and complete genomic analysis of porcine circovirus 2 (PCV2) in the Lesser Antilles island of St. Kitts: Identification of PCV2b‐PCV2d recombinants

First isolation and whole-genome characterization of a G9P[14] rotavirus strain from a diarrheic child in Egypt

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