Whole genome analysis of Enterobacter cloacae Rs-2 and screening of genes related to plant-growth promotion

Abstract

The genus Enterobacter is widely recognized for its biotechnology potential in improving soil environment and crop growth promoting. To further explore these biotechnological potentials, we sequenced and analyzed the whole genome of Enterobacter cloacae Rs-2. The analysis showed that the total length of Rs-2 genome was 6,965,070,514 bp, GC content was 55.80%; The annotation results of GO and COG databases showed that the genome contains a variety of growth-promoting genes, such as iscU, glnA, glnB (nitrogen xation); iucABCD (siderophore synthesis) and fepA, fcuA, fhuA, and pfeA, etc. (siderophore transport); ipdC (secreted IAA) and gcd, pqqBCDEF (dissolved phosphorus), etc. The application of Rs-2 as a soil inoculant in the pot experiment increased the average fresh weight of radish shoots and roots by 65.76% and 71.60%, respectively, the length of roots increased by 68.33%, and the chlorophyll content in the leaves also increased effectively. In addition, the total phosphorus content of shoots and roots was increased by 20.0% and 32.8%, respectively. This study proved the plant growthpromoting ability of Rs-2 at the molecular level through genetic screening and analysis, which provided guidance for the further improvement of the strain, and laid a foundation for its application in agricultural production.

Mitigation of Salt Stress in Rice by the Halotolerant Plant Growth-Promoting Bacterium Enterobacter asburiae D2

“…Instead, a D-cysteine desulfhydrase gene ( dcyD ) was found in the genome of D2. As a homolog of ACC deaminase, D-cysteine desulfhydrase has been reported to be responsible for the ACC deaminase activity of E. cloacae Rs-2 without the acdS gene [ 36 ].…”

Complete genome sequence of Nguyenibacter sp. L1, a phosphate solubilizing bacterium isolated from Lespedeza bicolor rhizosphere

“…Moreover, the protracted application of phosphorus fertilizers risks causing soil salinization and pollution, including groundwater contamination due to leaching and transpiration. These environmental quandaries threaten to diminish food production and potentially jeopardize human health ( Wang et al, 2023 ). Unlike nitrogen (N), which has volatile atmospheric forms such as N 2 and N 2 O, phosphorus lacks a soluble atmospheric supply ( Yang and Post, 2017 ).…”