Whole-genome analysis of a human rabies virus from Sri Lanka

Matsumoto, Takashi; Ahmed, Kawsar; Wimalaratne, Omala; Yamada, Kentaro; Nanayakkara, Susilakanthi; Perera, Devika; Karunanayake, Dushantha; Nishizono, Akira

doi:10.1007/s00705-010-0905-8

Cited by 25 publications

(25 citation statements)

References 54 publications

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“…To date, several methods, such as rapid amplification of cDNA (RACE) [22-24] and gene-walking approach have been employed to obtain the whole genome sequence, including the 5' and 3' untranslated regions (UTR). In the present study, a simple method of amplification and sequencing of full length of rabies virus genome was performed, according to reference 7.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of China rabies virus vaccine strain

Jiao

et al. 2011

Virol J

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BackgroundRabies virus (RV), the agent of rabies, can cause a severe encephalomyelitis in several species of mammals, including humans. As a human rabies vaccine strain employed in China, the genetic knowledge of the aG strain has not been fully studied. The main goal of the present study is to amplify the whole genome of aG strain, and genetic relationships between other vaccine strains and wild strains were analyzed.ResultsThe entire genome of human rabies virus vaccine strain aG employed in China was sequenced; this is the second rabies virus vaccine strain from China to be fully characterized. The overall organization and the length of the genome were similar to that of other lyssaviruses. The length of aG strain was 11925nt, comprising a leader sequence of 58nt, nucleoprotein (N) gene of 1353nt, phosphoprotein (P) gene of 894 nt, matrix protein (M) gene of 609nt, glycoprotein (G) gene of 1575nt, RNA-dependent RNA polymerase (RdRp,L) gene of 6384nt, and a trailer region of 70 nt. There was TGAAAAAAA (TGA7) consensus sequence in the end of each gene, except AGA7 at the end of G gene. There was AACAYYYCT consensus start signal at the beginning of each gene.ConclusionsIn this report, we analyzed the full genome of China human rabies vaccine strain aG. Our studies indicated that the genome of aG retained the basic characteristics of RV. At gene level, N was the most conserved among the five coding genes, indicating this gene is the most appropriate for quantitative genotype definition. The phylogenetic analysis of the N indicated the aG strain clustered most closely with Japanese and Russian rabies vaccine strains, suggesting that they may share the same ancestor; also, the aG strain did not share high homology with wild strains isolated from China, making it may not be the best vaccine strain, more research is needed to elucidate the genetic relationship among the RV circulating in China.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of China rabies virus vaccine strain

Jiao

et al. 2011

Virol J

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“…The primers used for complete genome sequencing of MLB2 were designed using reported sequences (9, 10). The terminal parts were amplified using a rapid amplification of cDNA ends kit, and the PCR products were purified and sequenced (13). …”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of an MLB2 Astrovirus from a Turkish Child with Diarrhea

et al. 2013

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“…Further, phylogeny using the partial nucleoprotein sequences must be used with caution as they are less reliable than using the complete nucleoprotein coding region [5]. Recently, Matsumoto et al [7] reported that the Sri Lankan isolates formed an independent lineage and did not cluster with Indian viruses while using full genome sequences for phylogeny. However the authors themselves suggested that the limited number of Indian viruses used in their study may not be from the southern part of India.…”

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confidence: 99%

Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene

et al. 2011

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Rabies is a highly fatal non-suppurative encephalomyelitis, caused by the Rabies virus. Dogs are the major reservoir of rabies in India and are the source of infection to other domestic animals. In this report, laboratory investigation and molecular characterization of isolates from two cows with paralytic rabies is described. Necropsy brain samples from the two cows were tested for the presence of rabies antigen using a fluorescent antibody test and the results were confirmed using RT-PCR. Rabies virus was successfully isolated from both the brain samples in a murine neuroblastoma cell line. The phylogenetic analysis of partial nucleoprotein gene sequences of these isolates showed them to be of a variant of Rabies virus which is closely related to the Sri Lankan Rabies virus lineage as previously reported. In addition, partial nucleoprotein genes of 19 more Rabies virus isolates from southern India were sequenced and of these 11 isolates were found to be closely related to the Sri Lankan lineage. The deduced amino acid sequences of the partial nucleoprotein of the Indian isolates were 96-99% identical to the Sri Lankan isolates. This investigation re-confirms the previous speculations that the Sri Lankan variant of the virus may still be actively transmitted by animals in India.

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Whole-genome analysis of a human rabies virus from Sri Lanka

Cited by 25 publications

References 54 publications

Molecular characterization of China rabies virus vaccine strain

Molecular characterization of China rabies virus vaccine strain

Complete Genome Sequence of an MLB2 Astrovirus from a Turkish Child with Diarrhea

Rabies in South Indian Cows: An evidence of Sri Lankan Rabies virus Variant Infection Based on the Analysis of Partial Nucleoprotein Gene

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