Whole-genome analysis and secondary metabolites production of a new strain Brevibacillus halotolerans 7WMA2: A potential biocontrol agent against fungal pathogens

Han, Ho Le; Jiang, Lingmin; Tran, Thi Ngoc Thu; Muhammad, Neak; Kim, Song‐Gun; Pham, Vinh Phu Tran; Ng, Yan Jer; Khoo, Kuan Shiong; Chew, Kit Wayne; Nguyen, Thi Dong Phuong

doi:10.1016/j.chemosphere.2022.136004

Cited by 11 publications

(5 citation statements)

References 55 publications

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“…In addition, eight of them lacked β-hemolytic activity, including isolates with high inhibitory activity against multidrug-resistant (MDR) bacteria (B. laterosporus) and isolates with activity against fungi (B. pumilus and B. altitudinis), which makes them promising candidates for potential future applications. The members of Bacillus and Brevibacillus genera obtained in this research are known for their ability to produce a variety of bioactive compounds with different activities such as antibacterial [29,30], antifungal [16,[31][32][33][34][35][36][37][38], antiviral [39][40][41], and pest control properties [42,43].…”

Section: Discussionmentioning

confidence: 99%

“…In our study, B. laterosporus X9433 is particularly remarkable for its broad spectrum of action against 10 of the 12 indicator bacteria (83%) including MRSA, MRSE, MRSP, L. monocytogenes, E. coli, and vancomycin-resistant Enterococcus isolates, although it did not show antifungal activity. This strain will be studied by whole-genome sequencing in future assays to determine whether it possesses genes encoding brevibacillin (antimicrobial lipopeptide effective against multidrug-resistant strains) [32,[53][54][55][56][57].…”

Section: Discussionmentioning

confidence: 99%

“…Authors such as Zhao et al 2021 [55] succeeded in identifying, purifying, and characterizing a brevibacillin 2V from B. laterosporus with strong antimicrobial activity against E. faecium, E. faecalis, and MRSA, while Yang et al 2018 [57] studied the antimicrobial mechanism of action of brevibacillin against sensitive S. aureus strains, although further studies are still needed to better understand its antimicrobial capacity. In addition, Le Han et al 2022 [32] isolated strains of B. halotolerans and B. laterosporus from marine sediment samples that produced thermostable and pH-tolerant antifungal compounds, which inhibited the growth of relevant pathogens such as Alternaria alternata, Candida albicans, Cladosporium sp., Trichophyton rubrum, Trichosporon pullulans, and F. oxysporum. The data obtained in this study corroborate the antibacterial capacity of B. laterosporus, which has also been previously detected in soil samples by our research group [27].…”

Section: Discussionmentioning

confidence: 99%

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Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Pino-Hurtado,

Fernández-Fernández,

Torres

et al. 2023

Antibiotics

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Antimicrobial resistance (AMR) is a serious threat to public health due to the lack of effective drugs to combat infectious diseases, which generates the need to search for new antimicrobial substances. In this study, the potential of soil as a source of antimicrobial-producing bacteria (APB) was investigated and the importance of the connection between education and science was emphasized, using service-learning methodologies. Sixty-one soil samples were collected, and 1220 bacterial isolates were recovered. Eighteen of these isolates showed antimicrobial activity against at least 1 of the 12 indicator bacteria tested (including multidrug-resistant and relevant pathogens). The 18 APB were identified by MALDI-TOF and 6 different genera (Bacillus, Brevibacillus, Lysinobacillus, Peribacillus, Streptomyces, and Advenella) and 10 species were identified. The 18 APB were tested for antifungal activity against four phytopathogenic fungi (Botritis cynerea, Lecanicillium fungicola, Trichoderma harzianum, and Cladobotryum mycophilum). Moreover, the antibiotic susceptibility of APB was tested using the disk-diffusion method as well as their β-hemolytic activity (important safety criteria for potential future applications). A total of 10 of the 18 APB were able to inhibit at least 50% of indicator bacteria tested, including methicillin-resistant Staphylococcus aureus (MRSA), among others. A total of 4 of the 18 APB (3 Bacillus pumilus and 1 Bacillus altitudinis) showed inhibitory activity against two of the four fungal pathogens tested (B. cinerea and L. fungicola), as well as against 5–7 of the 12 bacterial pathogen indicators; these 4 isolates showed susceptibility to the antibiotics tested and lacked β-hemolytic activity and were considered promising APB for use as potential biocontrol agents. In addition, one Brevibacillus laterosporus strain had activity against 83% of indicator bacteria tested including Escherichia coli, MRSA and other methicillin-resistant staphylococci, as well as vancomycin-resistant enterococci (but not against fungi). These results show that soil is a source of APB with relevant antibacterial and antifungal activities, and also emphasize the importance of education and science to raise public awareness of the AMR problem and the strategies to control it.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Pino-Hurtado,

Fernández-Fernández,

Torres

et al. 2023

Antibiotics

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show abstract

“…For biochemical characterization, the catalase and oxidase production were examined using 3% (v/v) H2O2 and 1% (w/v) tetramethylp-phenylenediamine dihydrochloride reagents, respectively [29]. The hydrolysis of Tweens 20, 40, 80, and casein were determined as described in the Cowan and Steel protocol [30].…”

Section: Physical Biochemical and Chemotaxonomic Analysismentioning

confidence: 99%

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Muhammad,

Avila,

Kim

2024

Preprint

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Background The phylum Bacteroidota represents a significant proportion of heterotrophic bacteria found in marine ecosystems. Members of the phylum Bacteroidota are actively involved in the degradation of biopolymers such as polysaccharides and proteins. Bacteroidota genomes exhibit a significant enrichment of various enzymes, including carbohydrate-active enzymes (CAZymes), carboxypeptidases, esterases, isomerases, peptidases, phosphatases, and sulfatases. The genus Marivirga, a member of the family Marivirgaceae within the phylum Bacteroidota, comprises six documented species. During a microbial diversity study, three novel Marivirga strains (BKB1-2T, ABR2-2, and BDSF4-3T) were isolated from the West Sea, Republic of Korea. Results To explore the taxonomic status and genomic characteristics of the novel isolates, we employed a polyphasic taxonomic approach, which included phylogenetic, chemotaxonomic and comprehensive genome analysis. The three isolates were Gram-stain-negative, aerobic, rod-shaped, moderately halophilic, and had a gliding motility. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the two isolates, BKB1-2T and BDSF4-3T, and the six reference strains were 70.5–76.5% for ANI and 18.1–25.7% for dDDH. Interestingly, the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the strains harbor genes for a comprehensive pathway for dissimilatory nitrate reduction to ammonium (DNRA), as well as other nitrogen pathways for the reduction of nitrite, nitric oxide, and nitrous oxide. Additionally, the antiSMASH analysis indicated that the strains contained three to eight biosynthetic gene clusters (BGCs) associated with the synthesis of secondary metabolites. Furthermore, the strains carried a high number of CAZyme ranging from 53 to 152, which was also demonstrated by an in vitro analysis of degradation of the polysaccharide cellulose, chitin, laminarin, starch, and xylan. Additionally, all the strains carried genes for the metabolism of heavy metals, and exhibited tolerance to heavy metals, with minimum inhibitory concentrations (MICs) in millimoles (mM) in ranges of Co2+ (3–6), Cu2+ (0.2–0.4), Ni2+ (3–5), Zn2+ (2–4), Mn2+ (20–50), and Hg2+ (0.3). Conclusions Based on polyphasic taxonomic approach, the three isolated strains represent two novel species names Marivirga arenosa sp. nov. (BKB1-2T = KCTC 82989T = InaCC B1618T), and Marivirga salinae sp. nov. (BDSF4-3T = KCTC 82973T = InaCC B1619T).

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“…There are also other intestinal fungi including Cryptococcus neoformans , Malassezia restricta elicits , Aspergillus sydowii , Trichosporon pullulans that can produce diverse virulence factors (capsule, mycotoxins, hypha, etc.) to intensify IBD [ 23 – 29 ]. Alike to beneficial bacteria, some gut probiotic fungi, such as Saccharomyces boulardii , can alleviate IBD through protecting intestinal barrier integrity, preventing pathogen colonization and invasion, and regulating anti-inflammatory levels [ 30 ].…”

Section: Intestinal Flora and Ibdmentioning

confidence: 99%

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

2022

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Inflammatory bowel disease is a heterogeneous intestinal inflammatory disorder, including ulcerative colitis (UC) and Crohn's disease (CD). Existing studies have shown that the pathogenesis of IBD is closely related to the host's genetic susceptibility, intestinal flora disturbance and mucosal immune abnormalities, etc. It is generally believed that there are complicated interactions between host immunity and intestinal microflora/microRNAs during the occurrence and progression of IBD. Intestinal flora is mainly composed of bacteria, fungi, viruses and helminths. These commensals are highly implicated in the maintenance of intestinal microenvironment homeostasis alone or in combination. MiRNA is an endogenous non-coding small RNA with a length of 20 to 22 nucleotides, which can perform a variety of biological functions by silencing or activating target genes through complementary pairing bonds. A large quantity of miRNAs are involved in intestinal inflammation, mucosal barrier integrity, autophagy, vesicle transportation and other small RNA alterations in IBD circumstance. In this review, the immunomodulatory roles of gut flora and microRNAs are updated in the occurrence and progression of IBD. Meanwhile, the gut flora and microRNA targeted therapeutic strategies as well as other immunomodulatory approaches including TNF-α monoclonal antibodies are also emphasized in the treatment of IBD.

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Whole-genome analysis and secondary metabolites production of a new strain Brevibacillus halotolerans 7WMA2: A potential biocontrol agent against fungal pathogens

Cited by 11 publications

References 55 publications

Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

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