Whole-genome analysis and secondary metabolites production of a new strain Brevibacillus halotolerans 7WMA2: A potential biocontrol agent against fungal pathogens

Han, Ho Le; Jiang, Lingmin; Tran, Thi Ngoc Thu; Muhammad, Neak; Kim, Song‐Gun; Pham, Vinh Phu Tran; Ng, Yan Jer; Khoo, Kuan Shiong; Chew, Kit Wayne; Nguyen, Thi Dong Phuong

doi:10.1016/j.chemosphere.2022.136004

Cited by 10 publications

(2 citation statements)

References 55 publications

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“…For biochemical characterization, the catalase and oxidase production were examined using 3% (v/v) H2O2 and 1% (w/v) tetramethylp-phenylenediamine dihydrochloride reagents, respectively [29]. The hydrolysis of Tweens 20, 40, 80, and casein were determined as described in the Cowan and Steel protocol [30].…”

Section: Physical Biochemical and Chemotaxonomic Analysismentioning

confidence: 99%

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Muhammad,

Avila,

Kim

2024

Preprint

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Background The phylum Bacteroidota represents a significant proportion of heterotrophic bacteria found in marine ecosystems. Members of the phylum Bacteroidota are actively involved in the degradation of biopolymers such as polysaccharides and proteins. Bacteroidota genomes exhibit a significant enrichment of various enzymes, including carbohydrate-active enzymes (CAZymes), carboxypeptidases, esterases, isomerases, peptidases, phosphatases, and sulfatases. The genus Marivirga, a member of the family Marivirgaceae within the phylum Bacteroidota, comprises six documented species. During a microbial diversity study, three novel Marivirga strains (BKB1-2T, ABR2-2, and BDSF4-3T) were isolated from the West Sea, Republic of Korea. Results To explore the taxonomic status and genomic characteristics of the novel isolates, we employed a polyphasic taxonomic approach, which included phylogenetic, chemotaxonomic and comprehensive genome analysis. The three isolates were Gram-stain-negative, aerobic, rod-shaped, moderately halophilic, and had a gliding motility. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the two isolates, BKB1-2T and BDSF4-3T, and the six reference strains were 70.5–76.5% for ANI and 18.1–25.7% for dDDH. Interestingly, the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the strains harbor genes for a comprehensive pathway for dissimilatory nitrate reduction to ammonium (DNRA), as well as other nitrogen pathways for the reduction of nitrite, nitric oxide, and nitrous oxide. Additionally, the antiSMASH analysis indicated that the strains contained three to eight biosynthetic gene clusters (BGCs) associated with the synthesis of secondary metabolites. Furthermore, the strains carried a high number of CAZyme ranging from 53 to 152, which was also demonstrated by an in vitro analysis of degradation of the polysaccharide cellulose, chitin, laminarin, starch, and xylan. Additionally, all the strains carried genes for the metabolism of heavy metals, and exhibited tolerance to heavy metals, with minimum inhibitory concentrations (MICs) in millimoles (mM) in ranges of Co2+ (3–6), Cu2+ (0.2–0.4), Ni2+ (3–5), Zn2+ (2–4), Mn2+ (20–50), and Hg2+ (0.3). Conclusions Based on polyphasic taxonomic approach, the three isolated strains represent two novel species names Marivirga arenosa sp. nov. (BKB1-2T = KCTC 82989T = InaCC B1618T), and Marivirga salinae sp. nov. (BDSF4-3T = KCTC 82973T = InaCC B1619T).

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Section: Physical Biochemical and Chemotaxonomic Analysismentioning

confidence: 99%

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Muhammad,

Avila,

Kim

2024

Preprint

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“…There are also other intestinal fungi including Cryptococcus neoformans , Malassezia restricta elicits , Aspergillus sydowii , Trichosporon pullulans that can produce diverse virulence factors (capsule, mycotoxins, hypha, etc.) to intensify IBD [ 23 – 29 ]. Alike to beneficial bacteria, some gut probiotic fungi, such as Saccharomyces boulardii , can alleviate IBD through protecting intestinal barrier integrity, preventing pathogen colonization and invasion, and regulating anti-inflammatory levels [ 30 ].…”

Section: Intestinal Flora and Ibdmentioning

confidence: 99%

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

2022

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Inflammatory bowel disease is a heterogeneous intestinal inflammatory disorder, including ulcerative colitis (UC) and Crohn's disease (CD). Existing studies have shown that the pathogenesis of IBD is closely related to the host's genetic susceptibility, intestinal flora disturbance and mucosal immune abnormalities, etc. It is generally believed that there are complicated interactions between host immunity and intestinal microflora/microRNAs during the occurrence and progression of IBD. Intestinal flora is mainly composed of bacteria, fungi, viruses and helminths. These commensals are highly implicated in the maintenance of intestinal microenvironment homeostasis alone or in combination. MiRNA is an endogenous non-coding small RNA with a length of 20 to 22 nucleotides, which can perform a variety of biological functions by silencing or activating target genes through complementary pairing bonds. A large quantity of miRNAs are involved in intestinal inflammation, mucosal barrier integrity, autophagy, vesicle transportation and other small RNA alterations in IBD circumstance. In this review, the immunomodulatory roles of gut flora and microRNAs are updated in the occurrence and progression of IBD. Meanwhile, the gut flora and microRNA targeted therapeutic strategies as well as other immunomodulatory approaches including TNF-α monoclonal antibodies are also emphasized in the treatment of IBD.

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Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens

Le Han,

Pham,

Kim

et al. 2023

Mol Biotechnol

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Whole-genome analysis and secondary metabolites production of a new strain Brevibacillus halotolerans 7WMA2: A potential biocontrol agent against fungal pathogens

Cited by 10 publications

References 55 publications

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Comparative genome analysis of the genus Marivirga and proposal of two novel marine species: Marivirga arenosa sp. nov., and Marivirga salinae sp. nov

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens

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