Whole Genome Amplification * Edited by S. Hughes and R. Lasken * Scion Publishing Ltd, Bloxham, UK; * 2005; ISBN 1-904842-07-0; 193 pp.; * Paperback.

Walker, Adrienne

doi:10.1093/bfgp/ell029

Cited by 1 publication

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“…The brightness values varied between 158 and 200, as depicted in Figure 1a, illustrating the visual diversity among each PC unit. Even though a single PC bag contained platelets from five blood donors of the same blood type and roughly 20% of the preservative solution [9], there were observable visual distinctions between each unit. In the analysis of biochemistry, the values for total protein, albumin, total bilirubin, creatine kinase, glucose, phosphate, AST, ALT, LDH, uric acid, BUN, creatinine, total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride, IgA, IgG, and IgM exhibited variabilities (Figure 1b).…”

Section: Identification Of Unit Variability In Visual Appearance Bioc...mentioning

confidence: 99%

Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute

Lee,

Chun,

Yoon

et al. 2023

JCM

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Due to the short storage period, large quantities of platelet concentrate (PC) are expiring. The expired PC cannot be injected into a blood vessel, but the activity of bioactive molecules, especially growth factors, is still preserved. In this paper, we organized a process to obtain a growth factor-rich bioproduct for use as a supplement in human cell culture by optimizing freezing, thawing, and sterilization conditions. Each unit of PC displayed visual differences, diverse biochemical values, and growth factor concentrations. To minimize lot-to-lot variation, we pooled a minimum of 10 PC units. The concentrations of growth factors were maximized through five freeze–thaw cycles for 12 h at −80 °C for freezing and for 5 min at 36 °C for thawing. We used a cell strainer with 40 µm pores, followed by a 0.45 μm filter and a 0.22 μm filter sequentially to sterilize the bioproduct with minimizing loss. The obtained growth factors remained stable for 4–6 h at room temperature (23 °C), 24 h at 4 °C, and 12 months at −80 °C. Cellular responses to the growth factor-rich bioproduct were tested with primary human renal proximal tubule epithelial cells. The cells exhibited a significantly increased growth rate, compared to the fetal bovine serum (FBS)-treated control group. The cells maintained their characteristic cuboidal shape, and stem cells and renal progenitor cells also preserved their genetic characteristics during culture. Therefore, the growth factor-rich bioproduct isolated from expired PC through our process can be used as a medium supplement to replace FBS in human cell culture for clinical application.

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Section: Identification Of Unit Variability In Visual Appearance Bioc...mentioning

confidence: 99%

Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute

Lee,

Chun,

Yoon

et al. 2023

JCM

View full text Add to dashboard Cite

show abstract

Whole Genome Amplification * Edited by S. Hughes and R. Lasken * Scion Publishing Ltd, Bloxham, UK; * 2005; ISBN 1-904842-07-0; 193 pp.; * Paperback.

Cited by 1 publication

References 0 publications

Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute

Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute

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