Whole Gene Family Expression and Drought Stress Regulation of Aquaporins

Alexandersson, Erik; Fraysse, Laure; Sjövall-Larsen, Sara; Gustavsson, Sofia; Fellert, Maria; Karlsson, Maria; Johanson, Urban; Kjellbom, Per

doi:10.1007/s11103-005-0352-1

Cited by 438 publications

(408 citation statements)

References 54 publications

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“…Roots and Is Induced by Anoxia Stress-Consistent with previous observations of microarray (31) and promoter-reporter fusion data (32), Q-PCR analysis of total RNA from various organs of 2-week old Arabidopsis shows that AtNIP2;1 transcript is predominantly expressed in roots compared with other organs (Fig. 1A).…”

Section: Atnip2;1 Is Expressed In the Vascular Tissue Of Arabidopsissupporting

confidence: 89%

“…As shown in Fig. 5, a low but detectable signal was observed for all NIPs in 10-day-old Arabidopsis seedlings, except AtNIP4;1, which is expressed at an exceedingly low level based on microarray and Q-PCR data (31). Although AtNIP1;1 showed a slight increase in expression in response to water logging, all other NIP transcripts showed little or no change in response to flooding or anoxia stress compared with AtNIP2;1 (Fig.…”

Section: Atnip2;1 Is Expressed In the Vascular Tissue Of Arabidopsismentioning

confidence: 80%

See 1 more Smart Citation

Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress

Choi

Roberts

2007

Journal of Biological Chemistry

161

126

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Nodulin 26 intrinsic proteins (NIPs) are plant-specific, highly conserved water and solute transport proteins with structural and functional homology to soybean nodulin 26. Arabidopsis thaliana contains nine NIP genes. In this study, it is shown that one of these, AtNIP2;1, is exquisitely sensitive to water logging and anoxia stress. Based on quantitative PCR and promoter::GUS experiments, AtNIP2;1 is expressed at a low basal level in the root tips and the vascular bundle of differentiated roots. Transcript levels are elevated acutely and rapidly upon water logging of root or leaf tissues, increasing 70-fold in roots within the 1st h of submersion. After this large initial increase, mRNA levels decline to steady state levels that remain over 10-fold higher by 6 h post-submersion. An even greater induction of AtNIP2;1 expression was observed upon anoxia challenge of Arabidopsis seedlings, with a 300-fold increase in AtNIP2;1 transcript observed by 2 h after the initiation of oxygen deprivation. Functional analysis of AtNIP2;1 expressed in Xenopus oocytes shows that the protein differs from soybean nodulin 26, showing minimal water and glycerol transport. Instead, AtNIP2;1 displays transport of lactic acid, with a preference for the protonated acidic form of this weak acid. Overall, the data suggest that AtNIP2;1 is an anaerobic-induced gene that encodes a lactic acid transporter and may play a role in adaptation to lactic fermentation under anaerobic stress.Major intrinsic proteins (MIPs) 2 are an ancient integral membrane channel protein family of water and uncharged solute transporters that have been found in nearly all living organisms. MIPs are especially prevalent and diverse in higher plants and have been classified into four monophyletic groups as follows: the plasma membrane intrinsic proteins (PIPs), the tonoplast intrinsic proteins, the nodulin 26-like intrinsic proteins (NIPs), and the small basic intrinsic proteins (1).NIPs are named for soybean nodulin 26 (Nod26) (2), which is the major protein component of the symbiosome membrane from nitrogen-fixing soybean root nodules (3, 4). Functional analyses indicate that Nod26 is an aquaglyceroporin with a low intrinsic water permeability and the ability to transport uncharged metabolites such as glycerol (4, 5) and has also been implicated in ammonia transport (6). It has become clear that NIPs represent a large, diverse family of aquaglyceroporins, with multiple members found in every sequenced higher plant genome (1,7,8). For example, among the 35 MIP genes in Arabidopsis thaliana (1), there are nine members of the NIP subfamily. Based on molecular modeling of the pore selectivity sequences, these nine NIPs are subdivided into the following two groups: NIP subgroup I proteins are encoded by NIP1;1, NIP1;2, NIP2;1, NIP3;1, NIP4;1, and NIP4;2, and NIP subgroup II proteins are encoded by NIP5;1, NIP6;1, and NIP7;1 (9).Analysis of NIP subgroup I proteins shows that they are more similar to soybean nodulin 26 in structure and function, with several showing aqu...

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Section: Atnip2;1 Is Expressed In the Vascular Tissue Of Arabidopsissupporting

confidence: 89%

Section: Atnip2;1 Is Expressed In the Vascular Tissue Of Arabidopsismentioning

confidence: 80%

Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress

Choi

Roberts

2007

Journal of Biological Chemistry

161

126

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“…npg The expression level of AtPIP1;1 increased in the aerial parts during the 12 h-drought treatment, but decreased in roots. In another study, similar expression regulation in AtPIPs was observed in response to drought [12]. However, due to different experimental conditions, some PIPs were different regulated.…”

Section: Introductionmentioning

confidence: 62%

“…We also observed that the transcript level of OsPIP2;4 in roots as well as some PIP genes in leaves of lowland rice remained unchanged under water deficit (Figure 3). These observations are not explained by the above-mentioned points, but in Arabidopsis, up-or down-regulation of PIP genes under water deficit was also described [11,12]. This diversity of PIP expression patterns in plants implies that the role of aquaporins in drought resistance is influenced by many factors.…”

Section: Roles Of Pips In Rice Drought Avoidancementioning

confidence: 96%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins (PIPs), was studied at both the protein and mRNA level in upland rice (Oryza sativa L. cv. Zhonghan 3) and lowland rice (Oryza sativa L. cv. Xiushui 63) when they were water stressed by treatment with 20% polyethylene glycol (PEG). Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1;2, Os-PIP1;3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1;2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid (ABA) level in upland rice but not in lowland rice under water deficit. Treatment with 60 µM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling pathways during water deficit.

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“…This differential expression could be plant-, aquaporin-, or stress-specific. Differential expression of aquaporins in response to different environmental stresses has been comprehensively evaluated in several plant species such as Arabidopsis, maize, poplar and B. rapa in the past years (Alexandersson et al 2005;Zhu et al 2005;Cohen et al 2013;Tao et al 2014). Regarding common bean, few aquaporins have been characterized to date.…”

Section: ; Magadum Et Al 2013) Duplicated Genes Could Undergo Pmentioning

confidence: 99%

Genome-wide identification and characterization of aquaporin gene family in common bean (Phaseolus vulgaris L.)

Ariani

Gepts

2015

Mol Genet Genomics

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Plant Aaquaporins are a large and diverse family of water channel proteins that are essential for several physiological processes in living organisms. in all living organisms. In plants, this family has the highest diversity of protein isoforms and substrate specificities compared to animals and bacteria. Numerous studies have linked plant aquaporins with a plethora of processes, such as nutrient acquisition, CO 2 transport, plant growth and development, and response to abiotic stresses.However, little is knowns about this protein family in common bean. Here, we present a genomewide identification of the aquaporin gene family in common bean (Phaseolus vulgaris L.), a legume crop essential for human nutrition. We identified 41 full-length coding aquaporin sequences in the common bean genome, divided by phylogenetic analysis into five sub-families (PIPs, TIPs, NIPs, SIPs and XIPs). Residues determining substrate specificity of aquaporins (i.e., NPA motifs and ar/R selectivity filter) seem conserved between common bean and other plant species, allowing inference of substrate specificity for these proteins. Thanks to the availability of RNA-sequencing datasets, expression levels in different organs and in leaves of wild and domesticated bean accessions were evaluated. Three aquaporins (PvTIP1;1, PvPIP2;4 and PvPIP1;2) have the overall highest mean expressions, with PvTIP1;1 having the highest expression among all aquaporins. We performed an EST database mining to identify drought responsive aquaporins in common bean. This analysis showed a significant increase in expression for PvTIP1;1 in drought stress conditions compared to well-watered environments. The pivotal role suggested for PvTIP1;1 in regulating water homeostasis and drought stress response in the common bean, should be verified by further field experimentation under drought stress.

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Whole Gene Family Expression and Drought Stress Regulation of Aquaporins

Cited by 438 publications

References 54 publications

Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress

Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Genome-wide identification and characterization of aquaporin gene family in common bean (Phaseolus vulgaris L.)

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